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  • Top
  • Abstract
  • Introduction
  • PPARα and FXR as mediators of the fasted and fed states in the liver
  • Opposing metabolic regulatory functions of PPARα and FXR in glucose and lipid metabolism
  • PPARα and FXR coordinately regulate autophagy
  • Regulation of the liver secretome by PPARα and FXR
  • Altered nuclear receptor signaling in extreme nutrient deprivation
  • Conclusions
  • Acknowledgments
  • Footnotes
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  • Version history
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Review Series Free access | 10.1172/JCI88893

Nutrient-sensing nuclear receptors PPARα and FXR control liver energy balance

Geoffrey A. Preidis,1 Kang Ho Kim,2 and David D. Moore2

1Section of Gastroenterology, Hepatology, and Nutrition, Department of Pediatrics, and

2Department of Molecular and Cellular Biology, Baylor College of Medicine, Houston, Texas, USA.

Address correspondence to: David D. Moore, Department of Molecular and Cellular Biology, 1 Baylor Plaza, Houston, Texas 77030, USA. Phone: 713.798.3313; E-mail: moore@bcm.edu.

Find articles by Preidis, G. in: JCI | PubMed | Google Scholar

1Section of Gastroenterology, Hepatology, and Nutrition, Department of Pediatrics, and

2Department of Molecular and Cellular Biology, Baylor College of Medicine, Houston, Texas, USA.

Address correspondence to: David D. Moore, Department of Molecular and Cellular Biology, 1 Baylor Plaza, Houston, Texas 77030, USA. Phone: 713.798.3313; E-mail: moore@bcm.edu.

Find articles by Kim, K. in: JCI | PubMed | Google Scholar

1Section of Gastroenterology, Hepatology, and Nutrition, Department of Pediatrics, and

2Department of Molecular and Cellular Biology, Baylor College of Medicine, Houston, Texas, USA.

Address correspondence to: David D. Moore, Department of Molecular and Cellular Biology, 1 Baylor Plaza, Houston, Texas 77030, USA. Phone: 713.798.3313; E-mail: moore@bcm.edu.

Find articles by Moore, D. in: JCI | PubMed | Google Scholar |

Published March 13, 2017 - More info

Published in Volume 127, Issue 4 on April 3, 2017
J Clin Invest. 2017;127(4):1193–1201. https://doi.org/10.1172/JCI88893.
Copyright © 2017, American Society for Clinical Investigation
Published March 13, 2017 - Version history
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Abstract

The nuclear receptors PPARα (encoded by NR1C1) and farnesoid X receptor (FXR, encoded by NR1H4) are activated in the liver in the fasted and fed state, respectively. PPARα activation induces fatty acid oxidation, while FXR controls bile acid homeostasis, but both nuclear receptors also regulate numerous other metabolic pathways relevant to liver energy balance. Here we review evidence that they function coordinately to control key nutrient pathways, including fatty acid oxidation and gluconeogenesis in the fasted state and lipogenesis and glycolysis in the fed state. We have also recently reported that these receptors have mutually antagonistic impacts on autophagy, which is induced by PPARα but suppressed by FXR. Secretion of multiple blood proteins is a major drain on liver energy and nutrient resources, and we present preliminary evidence that the liver secretome may be directly suppressed by PPARα, but induced by FXR. Finally, previous studies demonstrated a striking deficiency in bile acid levels in malnourished mice that is consistent with results in malnourished children. We present evidence that hepatic targets of PPARα and FXR are dysregulated in chronic undernutrition. We conclude that PPARα and FXR function coordinately to integrate liver energy balance.

Introduction

The liver is a central mediator of feeding and fasting transitions, pivoting from carbohydrate-based energy accumulation in the fed state to fatty acid oxidation–dependent (FAO-dependent) energy utilization in the fasted state (1). Nuclear receptors and their coregulators are central modulators of these transitions. PPARα is a well-known inducer of hepatic FAO in the fasted state (2). Although PPARα is thought to be activated by the high levels of fatty acids present in the fasted liver (3), the exact nature of the endogenous activation signal remains unknown (4).

PPARα activation also promotes gluconeogenesis (2). Hepatic farnesoid X receptor (FXR) is activated in the fed state by bile acids that return to the liver along with nutrients via the enterohepatic circulation. In addition to maintaining bile acid homeostasis, FXR exerts direct effects on metabolic pathways, including suppression of both gluconeogenesis and lipogenesis (5).

The opposite roles of PPARα and FXR in nutrient responses, exemplified by their opposite effects on gluconeogenesis, suggest both broad functional interactions and additional counteracting metabolic effects. At the most basic functional level, the two nutrient sensors regulate each other’s expression. In the fed state, bile acids activate expression of human PPARα via direct FXR transactivation, although this is apparently not conserved in mice (6). In the opposite direction, PPARα activation induces FXR mRNA expression in the fasted mouse liver (7). Thus, each nutrient state primes the other by increasing the expression of the appropriate nutrient sensor.

For additional metabolic effects we have recently shown that these receptors coordinately regulate another fundamental nutrient response in the liver, autophagy (8), as described in more detail below. Recent findings suggest the hepatic secretome is another potential target for complementary control of liver energy balance. Secretion is a very energy-intensive function of the liver, and we recently found that FXR activates the secretion of complement and coagulation factors in human hepatocytes (9). In contrast, earlier studies indicate that PPARα represses a broader range of secreted proteins in the livers of both mice (10) and cynomolgus monkeys (11). Here we discuss established overlapping and interdependent functional roles of PPARα and FXR in liver energy balance. We also provide preliminary support for predicted new roles for these nutrient sensors in regulation of the liver secretome and as potential therapeutic targets for chronic malnutrition. The focus on these two receptors should not be taken to exclude important roles for other nuclear receptors (12, 13), other nutrient-responsive transcription factors (14, 15), or their coregulators (16, 17) in central pathways of hepatic energy control. In addition, liver expression of both PPARα and FXR and many additional key metabolic genes is under independent circadian control, but the complex functional interactions of the circadian clock with nutrient response is beyond the scope of this Review.

PPARα and FXR as mediators of the fasted and fed states in the liver

The best-characterized function of PPARα is to mediate the induction of FAO in the fasted state (2). This is clearly evident from studies of Nr1c1–/– (Ppara–/–) mice, which show dramatic triglyceride accumulation in the fasted state due to loss of the potent PPARα stimulation of FAO in both peroxisomes and mitochondria (18–20). The idea that PPARα is activated in the fasted state by elevated levels of free fatty acids is appealing because it adds a dual role of the nutrients functioning as signals to induce their own utilization. However, PPARα activation in the fasted state does not depend on the fatty acids present in the diet (21), and there is evidence that the endogenous ligand is a product of hepatic lipogenesis (4). Direct analysis of lipids bound to liver PPARα suggested that the endogenous agonist is a relatively abundant phospholipid (22). Endocannabinoids, including oleylethanolamide, have also been identified as potential endogenous agonists (23), and the exact identity of the endogenous PPARα agonist(s) remains unclear. Interestingly, PPARα activity is reportedly suppressed in the fed state by a pathway dependent on mTORC1 activation of nuclear receptor co-repressor 1; inactivation of mTORC1 in the fasted state promotes appropriate PPARα activation (24).

The role of bile acids as endogenous agonist ligands for FXR is well established (25–27). Bile acid homeostasis is an important liver function that controls enterohepatic circulation of bile acids (5). Hepatic FXR is activated by the bile acids that return to the liver accompanied by the nutrients that they help to absorb. A recent report indicates that FXR is stabilized and activated by high glucose levels via O-GlcNAcylation. (28). In addition, FXR activation in the small intestine induces production of FGF-15 (FGF-19 in humans), which exerts insulin-like effects in the liver, including induction of protein and glycogen synthesis (29, 30). FXR activation is a consistent component of the fed state.

Opposing metabolic regulatory functions of PPARα and FXR in glucose and lipid metabolism

As noted above, their functions in opposite hepatic nutrient responses suggest that PPARα and FXR might have opposing effects on central metabolic pathways and liver energy balance. Among such pathways, gluconeogenesis is a key energy homeostasis pathway that has a particularly strong association with the fasting and feeding cycle. We (31) and others (32, 33) showed that FXR activation suppresses gluconeogenic genes via induction of the corepressor nuclear receptor SHP. In the opposite direction, hypoglycemia is a key phenotype of the fasted Ppara–/– mouse (19), and PPARα has been identified as a direct activator of a number of gluconeogenic genes (2, 34, 35). Although there are confounding reports (e.g., ref. 36), gluconeogenesis provides a clear example of complementary but opposing regulatory effects of PPARα and FXR.

The impact of the two nutrient sensors on pathways of glucose utilization in the fed state has been less studied. There is a report that treatment with the PPARα agonist fenofibrate decreased expression of glucokinase and pyruvate kinase (PK), which was accompanied by strongly increased expression of the key pyruvate dehydrogenase inhibitor PDK4 and decreased glycolytic flux (37). There are a number of other reports that PPARα can induce PDK4 in mouse and human hepatocytes (38, 39). Suppression of glycolysis by PPARα is consistent with its function in a fasted state. However, loss of FXR function in Nr1h4–/– (Fxr–/–) mice was associated with increased glycolytic flux in the refed state, indicating that FXR may also suppress glycolysis (40). This suppression was mainly attributed to decreased expression of liver PK (LPK) in the refed Fxr–/– livers, but FXR was also found to induce PDK4 expression in rat and human hepatocytes (41).

In lipid metabolism, the clearest impact of either of the two receptors is the activation of FAO by PPARα in the fasted state (2). Multiple studies with synthetic agonists and Ppara–/– mice support this conclusion, which is reinforced by the ability of PPARα agonists to induce the lipolytic metabolic hormone FGF-21 (42). The impact of FXR activation on FAO is less clear, but dietary treatment with the bile acid cholic acid reportedly inhibited primary PPARα targets, including hepatic mRNA expression of the FAO enzymes acyl-CoA oxidase (Acox1), bifunctional enzyme (Ehhadh), and thiolase (Acaa1a) (43). We found higher expression of FAO genes in Fxr–/– mice (31), and treatment of db/db mice with the FXR agonist GW4064 decreased ketogenesis (32). These results indicate that FAO provides another example of opposing effects of PPARα and FXR. However, FGF-21 has also been identified as a direct target of FXR (38, 44), and the ability of FXR activation to induce PDK4 (41) should also support FAO.

We (45) and others (46) have attributed the suppression of lipogenesis upon FXR activation to SHP induction and subsequent suppression of SREBP1c expression. Others have suggested that FXR inhibition of the well-known SREBP1c targets fatty acid synthase (Fasn) and acetyl-CoA carboxylase (Acc1) is independent of effects on SREBP1c (40). Inhibition of lipogenesis is consistent with the ability of FXR agonists to improve multiple aspects of the metabolic syndrome, not only in mouse models (31, 32), but also in humans (47); however, it is obviously inconsistent with the induction of this pathway in the fed state. There is less information on the impact of PPARα on de novo lipogenesis. There is a report that chronic fenofibrate treatment induces lipogenesis in concert with induction of FAO (37), a futile cycle that is not observed in response to fasting, and analysis of Fasn gene expression in fasted and fed Ppara–/– mice did not support a lipogenic effect (48). Instead, fenofibrate repressed lipogenic gene expression in high-fat– and high-cholesterol–fed LDL receptor knockout mice (49). Direct assessment of hepatic lipogenesis in hamsters fed a high-fructose diet (50) and, more importantly, in people with diabetes (51) showed the expected inhibitory effect of fenofibrate on this pathway. The impact of PPARα and FXR activation on primary pathways of energy metabolism is summarized in Figure 1. These effects are consistent with their expected roles as mediators of the fasted and feeding responses. Their effects are often opposite, particularly their opposing effects on gluconeogenesis and FAO; however, this functional dichotomy is not universal, since both appear to suppress lipogenesis, and they have similar effects on the expression of specific important metabolic regulators, notably PDK4.

Regulation of fundamental pathways of nutrient metabolism by PPARα and FXR.Figure 1

Regulation of fundamental pathways of nutrient metabolism by PPARα and FXR. The impact of FXR on glycolysis is not well understood. Green arrows indicate activation; red bars indicate repression. FFA, free fatty acid; PC, phosphatidylcholine; BA, bile acid.

PPARα and FXR coordinately regulate autophagy

Autophagy is a process by which essential nutrients can be recovered in times of deprivation via recycling of cellular components. The functions of PPARα and FXR as mediators of the fed and fasting responses led us to hypothesize that they could have opposing effects on autophagy. We tested this by treating fasted and fed wild-type, Ppara–/–, and Fxr–/– mice with the PPARα and FXR agonists GW7467 and GW4064. We found that the PPARα agonist induced autophagy, even when it was supposed to be off in the fed liver, while the FXR agonist suppressed it, even when it was supposed to be induced in the fasted liver (8). The two agonists had opposing effects on the expression of a large number of autophagy-related genes, and cistromics confirmed that such genes are highly enriched among both receptors’ primary targets. We also found that the normal physiologic induction of autophagy in the fasted state was significantly decreased in Ppara–/– livers. In Fxr–/– livers the expected inhibition of autophagy in the fed state was also blunted. These genetic results strongly reinforce the pharmacologic results and establish physiologic roles for both receptors as mediators of normal nutrient regulation of autophagy. The suppression of autophagy by FXR activation was confirmed by a companion manuscript (52).

Mechanistic studies showed that both PPARα and FXR could bind to DR-1 motifs (which were previously identified as positive response elements for PPARα) in the promoters of autophagy target genes, including genes encoding the autophagosome proteins microtubule-associated protein 1 light chain 3α and -β (LC3a and LC3b). FXR was not expected to bind to such sites, but this was confirmed by chromatin immunoprecipitation from wild-type and mutant livers treated with or without the agonists. In accordance with the observed transcriptional repression, FXR binding was associated with GW4064-dependent corepressor recruitment. PPARα and FXR directly compete for binding to the LC3a and LC3b promoter sites, with each agonist both increasing the binding of its own receptor and decreasing that of the other.

Regulation of the liver secretome by PPARα and FXR

A recent study of the genetic loss of FXR function in humans suggests an unexpected mechanism for PPARα and FXR to influence hepatic energy balance. We identified four patients from two families with loss-of-function mutations in the FXR (NR1H4) gene (9). Severe neonatal cholestasis was evident in all four patients, and two in one family were successfully treated with liver transplantation, while the other two died prior to one year of age. Cholestasis is expected from the role of FXR in bile acid control but is much more severe in FXR-deficient people than in Fxr–/– mice. All four patients also had coagulopathy. This unexpected phenotype was present from birth, well before the onset of severe liver symptoms, and therefore could not be attributed to end-stage liver disease. Four quite separate lines of evidence support a direct role for FXR as the complement and coagulation pathways. First, we showed that GW4064 induces multiple components of the complement and coagulation pathway in a human hepatocyte cell line (9), extending previous results with fibrinogen. Second, analysis of genome-wide FXR binding studies in mouse and human hepatocytes showed complement and coagulation third on the list of targeted pathways (53). Third, one study associated cholestasis due to diverse etiologies with increased coagulability (54). This was termed “paradoxical” due to the expected association of coagulopathy with severe liver disease but is exactly as expected for a direct transcriptional effect of FXR. Finally, this induction result was supported by a modest but significant increase in clotting rate in people with non-alcoholic steatohepatitis treated with obeticholic acid, a semisynthetic FXR agonist (47). Overall, it is clear that FXR directly regulates components of the complement and coagulation cascade.

There is extensive evidence that PPARα represses the same complement and coagulation pathways described above. Gene array profiling of the effects of the PPARα agonist ciprofibrate in the cynomolgus monkey showed the expected upregulation of FAO and other known targets but also strong downregulation of many complement and coagulation genes (11). In mice the complement and coagulation cascades were repressed by fasting (55) and were among the strongest negative targets of fenofibrate (10); they were also repressed by PPARα agonists in rats (56). Repression of fibrinogen gene expression by fenofibrate was lost in Ppara–/– mice (57). Complement genes are also repressed by fibrates in human hepatocytes (58), and fibrate treatments decrease fibrinogen levels in human serum (59, 60). The combination of either gemfibrozil (52) or fenofibrate (53) with warfarin markedly decreases clotting rates, although this has been attributed to pharmacodynamic effects related to displacement of warfarin from plasma albumin (61).

The metabolic functions of FXR and PPARα seem quite unrelated to coagulation; however, we have identified a potential linkage based on the idea that secretion is, in a sense, the inverse of autophagy: amino acids and energy in proteins are recovered by autophagy but are lost by secretion. Secretion is directly relevant to energy balance because it is arguably the most resource- and energy-intensive process in the liver. Since approximately 40% of all hepatocyte mRNAs encode secretory proteins (62), nearly half of the total protein produced by each hepatocyte is simply lost via secretion. This is a huge drain on resources. For the human liver, the daily total protein secretion is approximately 25 grams per day (63, 64), which corresponds to roughly half of the recommended daily protein consumption. Decreases in oxygen consumption upon cycloheximide treatment indicate that up to 10% of hepatocyte ATP goes to production of these secreted proteins (65). Thus, we extend the complement and coagulation pathways to the entire hepatocyte secretome and suggest that FXR licenses the process of secretion in the nutrient-rich fed state while PPARα spares resources by repressing it in the fasted state.

The regulation of the process of secretion in the fed and fasted states has been known for decades. Studies nearly 40 years ago showed that essentially all albumin mRNA is present on secretory rough endoplasmic reticulum polysomes in the fed rat liver, as expected, but the majority of these transcripts are released into the untranslated post-ribosomal pool in the fasted liver (66). In accord with this, human albumin production shows a two-fold daily fluctuation in production in the fed and fasted states (63), although this does not acutely affect serum albumin levels due to the approximately two-week half-life of the protein (64). Thus, the proposed PPARα and FXR effects represent the addition of a new transcriptional mechanism to a well-established regulatory effect. An intriguing aspect of this hypothesis is that it is completely independent of the diverse functions of the secreted proteins, which presumably are regulated by additional inputs. Instead we suggest that the impact of the secretome on hepatocyte energy balance is the basis for its apparent regulation by PPARα and FXR.

Available genome-wide profiling studies provide some support for the prediction that PPARα and FXR coordinately control the hepatocyte secretome. In one profile of fenofibrate effects in mice (67), nearly half (145/333) of a panel of liver-specific genes were altered by PPARα agonist treatment, and of these 110 (75.8%) were repressed (Figure 2A). As confirmed with analyses using Gene Ontology (www.geneontology.org) and Kyoto Encyclopedia of Genes and Genomes (KEGG; www.kegg.jp/) (Figure 2B), these suppressed genes are highly enriched for secreted proteins, including many in addition to the components of the complement and coagulation cascades. This enrichment of PPARα-repressed secretory targets is highly statistically significant.

Suppression of liver-specific gene expression by PPARα activation.Figure 2

Suppression of liver-specific gene expression by PPARα activation. (A) A list of 333 liver-specific genes from Pattern Gene Database (PaGenBase; http://bioinf.xmu.edu.cn/PaGenBase) was compared with an NCBI Gene Expression Omnibus fenofibrate-treated liver microarray (GEO GSE67796). The expression of 145 liver genes was found to be altered (P < 0.01), 110 (75.8%) of which were significantly downregulated by PPARα agonist treatment (hypergeometric test P = 1.89 × 10–38). (B) The 110 common genes were subjected to analysis using Database for Annotation, Visualization and Integrated Discovery (DAVID; https://david.ncifcrf.gov/), to address their cellular compartment (gene ontology–cellular compartment [GO-CC]), biological pathways (gene ontology–biological pathways [GO-BP]), and KEGG pathway associations. A majority of the genes encode secreted proteins located in extracellular space.

A further analysis compared transcripts affected by genetic loss of FXR with those altered by the PPARα agonists fenofibrate or Wy-14,643. The two comparisons identified more than 750 and 1,100 transcripts affected by both loss of FXR function and gain of PPARα function. In accordance with the opposite nutrient roles of the two nuclear receptors, concordant responses — either increased or decreased in both PPARα agonist and Fxr knockout — were much more common (67%) than discordant responses in the fenofibrate comparison (Figure 3A). Similarly, in the Wy-14,643 comparison, 61% of the common genes were altered in the same direction by PPARα activation and FXR inactivation. The increase in coordinate responses relative to discordant responses was highly statistically significant with the hypergeometric test. As expected, pathway analysis of the gene set representing PPARα activation and FXR inactivation highlighted FAO and additional PPARα targets relevant to energy balance, including mitochondrial function (Figure 3B). Additionally, secretome components were highly enriched in the gene set repressed by both PPARα agonists and Fxr knockout, as determined using both Gene Ontology and KEGG pathway analysis (Figure 3B). In the Gene Ontology analysis, nearly a quarter of the 412 genes in this category appeared in extracellular exosome (GO:0070062; 105 of 412; P = 8.92 × 10–12), extracellular region (GO:9995576; 134 of 412; P = 2.16 × 10–7) or extracellular vesicle (GO:1903461; 105 of 412; P = 1.31 × 10–11). Overall these results strongly support the predominance of the opposite effects of PPARα and FXR agonists but also leave open the possibilities of convergent effects, such as those observed with lipogenesis.

Overlapping target genes oppositely regulated by FXR and PPARα.Figure 3

Overlapping target genes oppositely regulated by FXR and PPARα. (A) Three microarray data sets (fenofibrate, GSE67796; Fxr knockout, GSE20599; Wy-14,643, GSE8295) were analyzed. Among a total of 14,026 genes, 2,634 (for fenofibrate), 2,028 (for Fxr knockout), and 4,671 genes (for Wy-14,643) were responsive to either PPARα activation (fenofibrate and Wy-14,643 treatment) or FXR genetic loss (P < 0.01), and many were responsive to both — fenofibrate treatment and Fxr knockout had 765 genes in common, and Fxr knockout and Wy-14,643 treatment had 1,104 genes in common. The majority of overlapping genes were regulated in the same direction (induced or repressed in both; 67.2% and 61.3% in each comparison). (B) DAVID analysis (https://david.ncifcrf.gov) of commonly regulated genes in the two comparisons of PPARα activation/FXR inhibition microarrays. Key aspects of increased or decreased genes were their locations in mitochondrion, membrane, endoplasmic reticulum, or extracellular space and functions as metabolic pathways, complement/coagulation, or fatty acid metabolism. Word cloud analysis (http://www.wordle.net) showed representation of the frequency of terms in the DAVID analysis outputs of the Gene Ontology Cellular Component (left) and KEGG pathway (right) (P < 0.05).

Altered nuclear receptor signaling in extreme nutrient deprivation

A more complete understanding of coordinate regulation of nutrient-sensing nuclear receptors has a potential impact on numerous pathologic conditions affected by liver energy imbalance. Although the current research focus is largely on overnutrition-associated metabolic syndrome, there is also great potential for relevance in child undernutrition. Globally, undernutrition contributes to half of all deaths of children under five years of age (68). When present in early childhood, undernutrition confers long-term deficits in growth potential and IQ, along with increased risk of obesity and related disorders later in life (69). Despite recent progress by the Millennium Development Goals (70), more than 113 million children worldwide remain underweight (71).

Similar to the acutely fasted state, chronic undernutrition requires the host to conserve and recycle amino acids and energy. Accordingly, signs of suppressed secretion and enhanced autophagy have been identified in protein calorie–restricted humans. Most notably, hypoalbuminemia is a hallmark of the severe, edematous form of undernutrition known as kwashiorkor (72). Likewise, dozens of studies have reported low plasma levels of complement proteins, in particular C3, in undernourished children (73). Suppressed secretion of complement likely contributes to the impaired cell-mediated immunity that is a feature of many forms of undernutrition (74).

Two other important secretory defects have been observed in chronic undernutrition. First, undernourished children have decreased plasma concentrations of coagulation factors with elevated prothrombin time (75–83). This coagulopathy often manifests as bruising but in rare cases can result in severe gastrointestinal hemorrhage (75, 81). Although the vitamin K–dependent factors II, VII, IX, and X are often decreased, many cases of coagulopathy in severe undernutrition are associated with decreased vitamin K–independent coagulation factors (80, 82) (71, 73) or are refractory to intravenous vitamin K therapy (75). More recently, coagulopathy was reported in a cohort of young adults with severe anorexia nervosa (84). Second, undernourished children have decreased concentrations of small intestinal conjugated bile acids (85–87), which leads to impaired lipid solubilization (88). Decreased bile acid secretion likely contributes to the observed dietary fat malabsorption (89–95), fat-soluble vitamin deficiencies (96), and small intestinal bacterial overgrowth (97–102). Decreased bile acids might also help explain why therapeutic refeeding often fails to result in sustained growth (103–105). Invasive small bowel sampling is no longer performed strictly for research purposes; however, a more recent study investigated fecal bile acids obtained from Malawian children with severe acute undernutrition who were admitted for inpatient nutritional rehabilitation. Nutritional recovery was associated with a nearly seven-fold increase in median concentration of conjugated bile acids in stool, compared with levels measured at hospital admission (106). Taken together, these findings suggest that undernourished individuals have decreased secretion of albumin, complement proteins, coagulation factors, and bile acids.

In addition to reduced secretion, there is also evidence that chronic protein calorie undernutrition upregulates autophagy. Numerous autophagosomes were identified with electron microscopy analysis of liver biopsy samples obtained from young adults with severe anorexia nervosa (84). Another recent study examined autophagic gene transcript and protein expression levels in skeletal muscle biopsies from otherwise healthy adults who had maintained a calorie-restricted diet for three or more years. Compared with sedentary individuals consuming Western diets, skeletal muscle from those with long-term calorie restriction contained numerous upregulated autophagic markers (107).

Overall, the decreased secretion and increased autophagy suggests that chronic protein calorie undernutrition in some respects parallels the acutely fasted state, with an imbalance between PPARα and FXR signaling. Fatty acids mobilized from peripheral fat stores might serve as endogenous PPARα ligands, whereas lack of FXR signaling might result from decreased bile acid synthesis. Indeed, we (108) and others (109) have reported striking decreases in conjugated bile acids in mouse models of protein energy undernutrition. Our metabolomic screen also revealed evidence of increased FAO and autophagy (108). However, recent studies using a rat model of undernutrition induced by a low-protein diet revealed hypoalbuminemia, loss of peroxisomes in hepatocytes, and impaired peroxisomal and mitochondrial function — effects that were partially ameliorated by the PPARα agonist fenofibrate (110). These data suggest a lack of PPARα signaling in this protein-deficient rat model, raising the possibility that endogenous ligands fail to activate PPARα and the resulting homeostatic effects. Alternatively, pathways other than PPARα signaling might contribute to FAO and energy-sparing suppression of secretion during chronic macronutrient deprivation.

Our quest to better understand nuclear receptor function and regulation in the undernourished host faces several challenges. These include integrating the coordinate activities of other nuclear receptors (such as vitamin D receptor) in settings of highly prevalent micronutrient deficiencies; evaluating liver secretory effects in the context of occult or overt liver diseases, including those related to undernutrition-associated steatosis or infections with helminth or viral pathogens; and navigating the challenges inherent to performing translational studies with extremely vulnerable patient populations. Nonetheless, we anticipate that defective PPARα or FXR signaling may be relevant to vexing questions, such as why some children are susceptible to hypoalbuminemia and kwashiorkor, whereas others develop marasmus, the non-edematous form of undernutrition. Proper nuclear receptor activation may also provide an opportunity to improve refeeding practices, reduce mortality, enhance catch-up growth, and decrease long-term metabolic complications of early-life undernutrition.

Conclusions

Gemfibrozil and fenofibrate are the two fibrates currently approved for use in the US. Based on their ability to lower serum triglycerides and decrease cardiovascular events, their usage approximately doubled in the US from 2004 to 2009 (111). In 2010, results from the Action to Control Cardiovascular Risk in Diabetes (ACCORD) trial confirmed that the addition of fenofibrate to simvastatin decreased cardiovascular risk in the subset of simvastatin-treated patients with relatively high triglycerides and low HDL (112), and it is likely that fibrate usage will continue to increase. The FXR Ligand Obeticholic Acid for Non-cirrhotic, Non-alcoholic Steatohepatitis (FLINT) trial recently showed that the bile acid derivative obeticholic acid improves multiple parameters of metabolic syndrome (47). Thus, obeticholic acid, which was very recently approved for the treatment of cholestatic liver disease (113), may also come into common use for treating metabolic syndrome.

The common beneficial impact of both PPARα and FXR agonists on aspects of metabolic syndrome seems inconsistent with many of the results outlined above. How can activation of two receptors with apparently opposite metabolic functions have similar beneficial effects? One possibility relates to the ability of both to suppress lipogenesis. As suggested by the late Denis McGarry, increased lipogenesis in the insulin-resistant liver can drive a vicious cycle in which increased steatosis reinforces insulin resistance, which in turn drives even more steatosis (114). In response to activation of several nuclear receptors, including FXR and possibly PPARα, this cycle may be reversed (115). In this model, inhibition of lipogenesis in response to receptor activation improves fatty liver and promotes insulin sensitivity, which in turn further suppresses lipogenesis and propagates as a beneficial cycle. For PPARα, this model is consistent with results in mice (116) and with some reports in humans that suggest insulin sensitivity is improved in response to fenofibrate treatment (117–120). However, the model is not supported by other clinical studies that have not shown either decreased steatosis (121) or increased insulin sensitivity in humans in response to fenofibrate (121–124).

There are still hundreds of genes that respond in the same direction to activation of the two nuclear receptors that may provide additional specific mechanisms. More broadly, we conclude that the preponderance of opposing effects of PPARα and FXR (Figure 3) correlates with their complementary activation in the fed and fasted states. This means that the two receptors do not simply cancel each other’s effects. Instead, in specific pathways such as activation and suppression of gluconeogenesis and autophagy in the fasted and fed states, the two nutrient sensors function coordinately in the appropriate nutrient contexts to appropriately regulate metabolic flux and energy balance. This suggests that specific activation of each in the correct portion of the daily cycle could promote proper metabolic balance via quite distinct mechanisms. Perhaps combinations of PPARα and FXR agonists would have reinforcing or synergistic beneficial effects if it were pharmacologically possible for each to target only the appropriate state.

Acknowledgments

This work was supported by NIDDK grant U24 DK097748. DDM is supported by the Robert R.P. Doherty Jr. Welch Chair in Science (grant Q-0022). GAP is supported by NIH training grant T32DK007664; by the US Public Health Service grant P30DK56338, which funds the Texas Medical Center Digestive Disease Center; and by the North American Society for Pediatric Gastroenterology, Hepatology and Nutrition Foundation/Nestle Nutrition Research Young Investigator Development Award.

Footnotes

Conflict of interest: The authors have declared that no conflict of interest exists.

Reference information:J Clin Invest. 2017;127(4):1193–1201.https://doi.org/10.1172/JCI88893.

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Version history
  • Version 1 (March 13, 2017): Electronic publication
  • Version 2 (April 3, 2017): Print issue publication

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Go to

  • Top
  • Abstract
  • Introduction
  • PPARα and FXR as mediators of the fasted and fed states in the liver
  • Opposing metabolic regulatory functions of PPARα and FXR in glucose and lipid metabolism
  • PPARα and FXR coordinately regulate autophagy
  • Regulation of the liver secretome by PPARα and FXR
  • Altered nuclear receptor signaling in extreme nutrient deprivation
  • Conclusions
  • Acknowledgments
  • Footnotes
  • References
  • Version history
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