Go to JCI Insight
  • About
  • Editors
  • Consulting Editors
  • For authors
  • Publication ethics
  • Publication alerts by email
  • Advertising
  • Job board
  • Contact
  • Clinical Research and Public Health
  • Current issue
  • Past issues
  • By specialty
    • COVID-19
    • Cardiology
    • Gastroenterology
    • Immunology
    • Metabolism
    • Nephrology
    • Neuroscience
    • Oncology
    • Pulmonology
    • Vascular biology
    • All ...
  • Videos
    • Conversations with Giants in Medicine
    • Video Abstracts
  • Reviews
    • View all reviews ...
    • Complement Biology and Therapeutics (May 2025)
    • Evolving insights into MASLD and MASH pathogenesis and treatment (Apr 2025)
    • Microbiome in Health and Disease (Feb 2025)
    • Substance Use Disorders (Oct 2024)
    • Clonal Hematopoiesis (Oct 2024)
    • Sex Differences in Medicine (Sep 2024)
    • Vascular Malformations (Apr 2024)
    • View all review series ...
  • Viewpoint
  • Collections
    • In-Press Preview
    • Clinical Research and Public Health
    • Research Letters
    • Letters to the Editor
    • Editorials
    • Commentaries
    • Editor's notes
    • Reviews
    • Viewpoints
    • 100th anniversary
    • Top read articles

  • Current issue
  • Past issues
  • Specialties
  • Reviews
  • Review series
  • Conversations with Giants in Medicine
  • Video Abstracts
  • In-Press Preview
  • Clinical Research and Public Health
  • Research Letters
  • Letters to the Editor
  • Editorials
  • Commentaries
  • Editor's notes
  • Reviews
  • Viewpoints
  • 100th anniversary
  • Top read articles
  • About
  • Editors
  • Consulting Editors
  • For authors
  • Publication ethics
  • Publication alerts by email
  • Advertising
  • Job board
  • Contact
Top
  • View PDF
  • Download citation information
  • Send a comment
  • Terms of use
  • Standard abbreviations
  • Need help? Email the journal
  • Top
  • Abstract
  • Inflammation and its resolution: role of maresins
  • Nonresolving inflammation in nonalcoholic steatohepatitis
  • MaR1: a novel agonist for RORα?
  • Feed-forward loop for the activation of RORα
  • Future directions
  • Acknowledgments
  • Footnotes
  • References
  • Version history
  • Article usage
  • Citations to this article

Advertisement

Commentary Free access | 10.1172/JCI127583

Resolving inflammation in nonalcoholic steatohepatitis

Matthew Spite

Center for Experimental Therapeutics and Reperfusion Injury, Department of Anesthesiology, Perioperative and Pain Medicine, Brigham and Women’s Hospital and Harvard Medical School, Boston, Massachusetts, USA.

Address correspondence to: Matthew Spite, Hale Building for Transformative Medicine, 60 Fenwood Road, Room 3016K, Boston, Massachusetts 02115, USA. Phone: 617.525.5133; Email: mspite@bwh.harvard.edu.

Find articles by Spite, M. in: PubMed | Google Scholar |

Published March 11, 2019 - More info

Published in Volume 129, Issue 4 on April 1, 2019
J Clin Invest. 2019;129(4):1524–1526. https://doi.org/10.1172/JCI127583.
© 2019 American Society for Clinical Investigation
Published March 11, 2019 - Version history
View PDF

Related article:

A maresin 1/RORα/12-lipoxygenase autoregulatory circuit prevents inflammation and progression of nonalcoholic steatohepatitis
Yong-Hyun Han, … , Bong-Jin Lee, Mi-Ock Lee
Yong-Hyun Han, … , Bong-Jin Lee, Mi-Ock Lee
Research Article Hepatology Inflammation

A maresin 1/RORα/12-lipoxygenase autoregulatory circuit prevents inflammation and progression of nonalcoholic steatohepatitis

  • Text
  • PDF
Abstract

Retinoic acid–related orphan receptor α (RORα) is considered a key regulator of polarization in liver macrophages that is closely related to nonalcoholic steatohepatitis (NASH) pathogenesis. However, hepatic microenvironments that support the function of RORα as a polarity regulator were largely unknown. Here, we identified maresin 1 (MaR1), a docosahexaenoic acid (DHA) metabolite with a function of specialized proresolving mediator, as an endogenous ligand of RORα. MaR1 enhanced the expression and transcriptional activity of RORα and thereby increased the M2 polarity of liver macrophages. Administration of MaR1 protected mice from high-fat diet–induced NASH in a RORα-dependent manner. Surprisingly, RORα increased the level of MaR1 through transcriptional induction of 12-lipoxygenase (12-LOX), a key enzyme in MaR1 biosynthesis. Furthermore, we demonstrated that modulation of 12-LOX activity enhanced the protective function of DHA against NASH. Together, these results suggest that the MaR1/RORα/12-LOX autoregulatory circuit could offer potential therapeutic strategies for curing NASH.

Authors

Yong-Hyun Han, Kyong-Oh Shin, Ju-Yeon Kim, Daulat B. Khadka, Hyeon-Ji Kim, Yong-Moon Lee, Won-Jea Cho, Ji-Young Cha, Bong-Jin Lee, Mi-Ock Lee

×

Abstract

Chronic unresolved inflammation contributes to the development of nonalcoholic steatohepatitis (NASH), a disorder characterized by lipotoxicity, fibrosis, and progressive liver dysfunction. In this issue of the JCI, Han et al. report that maresin 1 (MaR1), a proresolving lipid mediator, mitigates NASH by reprograming macrophages to an antiinflammatory phenotype. Mechanistically, they identified retinoic acid–related orphan receptor α (RORα) as both a target and autocrine regulator of MaR1 production. Because NASH is associated with many widely occurring metabolic diseases, including obesity and type 2 diabetes, identification of this endogenous protective pathway could have broad therapeutic implications.

Inflammation and its resolution: role of maresins

Acute inflammation is a temporally coordinated host response to invading pathogens and tissue injury. Instructed by endogenous mediators, distinct leukocyte subsets sequentially follow defined routes, carry out specialized tasks, and ultimately clear from the tissue so that its function can be restored. Proinflammatory mediators, such as chemokines and lipid mediators (e.g., leukotrienes, prostaglandins), promote vascular permeability, amplify leukocyte infiltration, and stimulate the release of substances that assist in pathogen eradication. Resolution of inflammation begins with the biosynthesis of mediators that terminate neutrophil infiltration and proinflammatory mediator production to prevent excessive damage to healthy tissue. These specialized proresolving mediators (SPMs), such as the maresins, also stimulate phagocytes to clear dead cells and bacteria, and they actively engage tissue reparative programs (1). Thus, SPMs are host-protective mediators that temper inflammation without causing immunosuppression.

Maresins (macrophage mediators in resolving inflammation) were originally discovered by Serhan et al. in resolving inflammatory exudates as novel proresolving molecules (2). Their complete structures with stereochemical assignments have been systematically elucidated, and the mechanisms of their biosynthesis have been defined. Maresin 1 (MaR1) is biosynthesized from docosahexaenoic acid (DHA) via 12-lipoxygenase–mediated (12-LOX–mediated) production of a 14-hydroperoxy intermediate that undergoes enzymatic epoxidation to form 13S, 14S-epoxy maresin (eMaR) (2, 3). This epoxide is enzymatically converted to MaR1 (7R, 14S-dihydroxy-4Z,8E,10E,12Z,16Z,19Z-docosahexaenoic acid) and MaR2, or can be conjugated with glutathione to produce the newly characterized cysteinyl SPM (2–5). Maresins blunt neutrophil chemotaxis and promote macrophage efferocytosis in animal models of acute inflammation. They have direct actions on human macrophages, are organ protective, and stimulate tissue regeneration (2, 6, 7). Earlier studies have also determined that MaR1, as well as other SPMs such as resolvins and protectins, resolve chronic inflammation and improve systemic metabolism and hepatic steatosis in mouse models of obesity and type 2 diabetes (8–11). In this issue, Han et al. add new insights into the regulation of MaR1 biosynthesis in obesity and identify downstream pathways engaged by MaR1 in macrophages that could, in part, underlie its protective actions (12).

Nonresolving inflammation in nonalcoholic steatohepatitis

While acute inflammation normally resolves, accumulating evidence indicates that maladaptive unresolved inflammation contributes to several chronic diseases, including nonalcoholic steatohepatitis (NASH) (13). The development of NASH is associated with diseases of altered systemic metabolism, such as type 2 diabetes, obesity, and hyperlipidemia. Lipotoxicity in hepatocytes occurs as a result of an imbalance between lipogenesis, secretion, and fatty acid β-oxidation, and can lead to apoptosis and necrosis. Release of endogenous damage-associated molecular patterns (DAMPs) initiates a sterile inflammatory response characterized by robust proinflammatory cytokine production and accumulation of leukocytes (e.g., neutrophils, macrophages, dendritic cells, or Th17 cells). Macrophages polarized to a proinflammatory state play a critical role in the progression of NASH, both in perpetuating chronic inflammatory signaling via the production of inflammatory cytokines (e.g., TNF-α and IL-1β) and in promoting fibrosis via the release of profibrotic cytokines (i.e., TGF-β1) (13). In contrast, polarization of liver macrophages to an antiinflammatory (i.e., M2) phenotype protects against the progression of NASH.

Previous studies determined a critical role of the nuclear receptor retinoic acid–related orphan receptor α (RORα) in the regulation of lipid metabolism and inflammation in NASH (14–16). In a previous study, Han et al. reported that RORα is important for polarizing liver macrophages to an antiinflammatory phenotype during NASH (17). In their present study, Han et al. hypothesized that, because of the interplay between RORα and lipid metabolism and inflammation in the liver, lipid-derived mediators may regulate RORα activation for resolution. Since DHA has been shown to improve NASH and chronic inflammation in several other contexts (11), they questioned whether RORα contributes to these protective actions. In initial studies, they found that DHA increased expression of RORα and that this was associated with increased M2 macrophage polarization. Similar results were obtained in fat-1 transgenic mice, which have high endogenous levels of DHA in the liver. Interestingly, the regulation of macrophage polarization by DHA was lost in macrophages from mice with myeloid-specific deficiency of RORα (RORα-MKO), establishing that activation of RORα mediates, in part, the role of DHA in this context.

MaR1: a novel agonist for RORα?

Earlier studies reported that DHA activates retinoid X receptor (18). Han et al. questioned whether DHA is also a ligand for RORα. While DHA increased a RORα reporter gene, DHA itself was not a direct ligand for RORα based on failure to recruit a coactivator peptide as compared with a synthetic RORα agonist. Because DHA is known to be converted in macrophages to bioactive lipid mediators that resolve inflammation (1), the authors tested whether specific SPMs regulated RORα in macrophages. From these studies, the authors determined that MaR1, but not other SPMs (i.e., resolvin D1), increased expression of RORα coincident with an increase in M2 macrophage polarization, which was both time- and dose-dependent. Of note, these results are consistent with prior studies showing that MaR1 regulates a proresolving macrophage phenotype (3). The regulation of M2 polarization was abolished by genetic deficiency of Rora. Consistent with activation of RORα, MaR1 promoted binding of coactivator p300 concomitant with decreased binding of NCoR1, a corepressor of RORα. Structure function analysis demonstrated that several other closely related SPMs (e.g., RvD1, RvD2, and protectin D1) did not modulate RORα and that activation of RORα by MaR1 was specific, as other nuclear receptors including RORβ, RORγ, retinoid X receptor α, peroxisome proliferator-activated receptor γ, and liver x receptor α, were not activated by MaR1. Like DHA and consistent with prior studies, treatment of obese mice with MaR1 prevented the development of NASH, as evidenced by reduced liver weight, hepatic triglyceride levels, markers of liver injury, and expression of profibrotic proteins. Similarly, MaR1 treatment increased the appearance of M2 polarized macrophages in the liver of obese mice. Notably, these protective effects were lost in RORα-MKO mice, establishing causality in the activation of this pathway by MaR1 in vivo.

The activation of RORα by MaR1 is surprising because most SPMs, as well as other lipid mediators, elicit their biological actions by binding to and activation of plasma membrane G protein–coupled receptors, rather than nuclear receptors (19, 20). Nonetheless, using surface plasmon resonance, fluorescence resonance energy transfer assays, and molecular modeling, Han et al. systematically demonstrated that MaR1 is likely an agonist of RORα. Transcriptome profiling of macrophages stimulated with MaR1 showed some overlap with genes induced by a synthetic RORα agonist (i.e., SR1078), although it should be noted that MaR1 distinctly regulated a large subset of genes as well. In addition, MaR1 is known to be metabolized in macrophages to further downstream products (e.g., 14-oxoMaR1) (21). Whether these metabolites or MaR1 itself serve as the actual ligand(s) for RORα remains to be determined. Moreover, acute activation of macrophage phagocytosis by MaR1 was RORα-independent and occurred at much lower concentrations and shorter time periods than that needed to activate RORα (10 nM vs. >50 nM). Thus, given that stimulation of macrophage phagocytosis is a key biological function of MaR1 during resolution of acute inflammation (1, 2), these results suggest that an as-yet-unidentified receptor may distinctly mediate this process.

Feed-forward loop for the activation of RORα

As noted above, several chronic diseases such as NASH are associated with nonresolving inflammation. Given that the symptoms of NASH could be partially alleviated by MaR1, Han et al. next questioned whether its development is associated with an impairment in MaR1 biosynthesis. To this end, they established that the expression of key MaR1 biosynthetic enzyme ALOX12 (encoding 12-LOX) is inversely associated with NASH in humans. In mice, development of NASH was associated with decreased hepatic levels of MaR1. Expression of Alox12 was decreased in RORα-MKO mice, indicating that, in addition to serving as a target for MaR1, RORα may also regulate the MaR1 biosynthetic machinery. To test this, the authors treated mice with SR1078 and found that MaR1 increased in WT mice. Similarly, treatment of mice with DHA increased MaR1 levels in WT mice but not in RORα-MKO mice. Further experiments determined that Alox12 is directly regulated by RORα binding and overexpression of Alox12 together with DHA treatment protected mice from NASH, whereas DHA was ineffective in mice treated with a 12-LOX inhibitor. Altogether, these results establish a feed-forward autoregulatory loop in which MaR1 activates RORα in macrophages, which in turn further increases transcription of Alox12 to potentiate MaR1 production (see Figure 1). It should be noted that MaR1 also has direct protective actions on hepatocytes, and thus local macrophage-derived MaR1 could potentially dampen steatosis via these additional cellular targets as well (22).

In macrophages, 12-LOX converts DHA to a 14-hydroperoxy DHA (hpDHA) intermeFigure 1

In macrophages, 12-LOX converts DHA to a 14-hydroperoxy DHA (hpDHA) intermediate that undergoes enzymatic epoxidation and hydrolysis to form MaR1. Han et al. found that MaR1 binds to and activates RORα, which leads to an antiinflammatory macrophage phenotype that blunts the progression of NASH. In addition, autoregulation of 12-LOX expression by RORα establishes a positive feed-forward circuit. Biological actions of MaR1 that are key during resolution of inflammation, such as macrophage phagocytosis, were found to be independent of RORα. Illustration by Rachel Davidowitz.

Future directions

In summary, the study by Han et al. adds new insight into the endogenous biosynthesis and actions of MaR1. Because RORα plays numerous roles in cellular metabolism and inflammation, it will be interesting to determine the role of this pathway in regulating other protective actions of MaR1 (23). For example, it has been reported that mice deficient in Rora develop severe atherosclerosis, a progressive chronic inflammatory disease that is also improved by MaR1 (24, 25). Nonetheless, key biological roles of MaR1 during resolution, such as enhancement of macrophage phagocytosis, were found to be independent of RORα, suggesting that previously undescribed receptors are likely to also contribute to these well-characterized roles in vivo.

Acknowledgments

The author’s laboratory is supported in part by NIH grants HL106173 and GM095467.

Address correspondence to: Matthew Spite, Hale Building for Transformative Medicine, 60 Fenwood Road, Room 3016K, Boston, Massachusetts 02115, USA. Phone: 617.525.5133; Email: mspite@bwh.harvard.edu.

Footnotes

Conflict of interest: The author has declared that no conflict of interest exists.

Copyright: © 2019 American Society for Clinical Investigation

Reference information: J Clin Invest. 2019;129(4):1524–1526. https://doi.org/10.1172/JCI127583.

See the related article at A maresin 1/RORα/12-lipoxygenase autoregulatory circuit prevents inflammation and progression of nonalcoholic steatohepatitis.

References
  1. Serhan CN, Levy BD. Resolvins in inflammation: emergence of the pro-resolving superfamily of mediators. J Clin Invest. 2018;128(7):2657–2669.
    View this article via: JCI PubMed CrossRef Google Scholar
  2. Serhan CN, et al. Maresins: novel macrophage mediators with potent antiinflammatory and proresolving actions. J Exp Med. 2009;206(1):15–23.
    View this article via: PubMed CrossRef Google Scholar
  3. Dalli J, et al. The novel 13S,14S-epoxy-maresin is converted by human macrophages to maresin 1 (MaR1), inhibits leukotriene A4 hydrolase (LTA4H), and shifts macrophage phenotype. FASEB J. 2013;27(7):2573–2583.
    View this article via: PubMed CrossRef Google Scholar
  4. Dalli J, Chiang N, Serhan CN. Identification of 14-series sulfido-conjugated mediators that promote resolution of infection and organ protection. Proc Natl Acad Sci U S A. 2014;111(44):E4753–E4761.
    View this article via: PubMed CrossRef Google Scholar
  5. Deng B, et al. Maresin biosynthesis and identification of maresin 2, a new anti-inflammatory and pro-resolving mediator from human macrophages. PLoS One. 2014;9(7):e102362.
    View this article via: PubMed CrossRef Google Scholar
  6. Serhan CN, et al. Macrophage proresolving mediator maresin 1 stimulates tissue regeneration and controls pain. FASEB J. 2012;26(4):1755–1765.
    View this article via: PubMed CrossRef Google Scholar
  7. Abdulnour RE, et al. Maresin 1 biosynthesis during platelet-neutrophil interactions is organ-protective. Proc Natl Acad Sci U S A. 2014;111(46):16526–16531.
    View this article via: PubMed CrossRef Google Scholar
  8. Jung TW, Kim HC, Abd El-Aty AM, Jeong JH. Maresin 1 attenuates NAFLD by suppression of endoplasmic reticulum stress via AMPK-SERCA2b pathway. J Biol Chem. 2018;293(11):3981–3988.
    View this article via: PubMed CrossRef Google Scholar
  9. Laiglesia LM, et al. Maresin 1 mitigates liver steatosis in ob/ob and diet-induced obese mice. Int J Obes (Lond). 2018;42(3):572–579.
    View this article via: PubMed CrossRef Google Scholar
  10. Martínez-Fernández L, et al. Maresin 1 improves insulin sensitivity and attenuates adipose tissue inflammation in ob/ob and diet-induced obese mice. FASEB J. 2017;31(5):2135–2145.
    View this article via: PubMed CrossRef Google Scholar
  11. Spite M, Clària J, Serhan CN. Resolvins, specialized proresolving lipid mediators, and their potential roles in metabolic diseases. Cell Metab. 2014;19(1):21–36.
    View this article via: PubMed CrossRef Google Scholar
  12. Han YH, et al. A maresin 1/RORα/12-lipoxygenase autoregulatory circuit prevents inflammation and progression of nonalcoholic steatohepatitis. J Clin Invest. 2019;129(4):1684–1698.
    View this article via: JCI CrossRef Google Scholar
  13. Koyama Y, Brenner DA. Liver inflammation and fibrosis. J Clin Invest. 2017;127(1):55–64.
    View this article via: JCI PubMed CrossRef Google Scholar
  14. Kang HS, et al. Transcriptional profiling reveals a role for RORalpha in regulating gene expression in obesity-associated inflammation and hepatic steatosis. Physiol Genomics. 2011;43(13):818–828.
    View this article via: PubMed CrossRef Google Scholar
  15. Kim HJ, et al. Liver-specific deletion of RORα aggravates diet-induced nonalcoholic steatohepatitis by inducing mitochondrial dysfunction. Sci Rep. 2017;7(1):16041.
    View this article via: PubMed CrossRef Google Scholar
  16. Lau P, Fitzsimmons RL, Raichur S, Wang SC, Lechtken A, Muscat GE. The orphan nuclear receptor, RORα, regulates gene expression that controls lipid metabolism: staggerer (SG/SG) mice are resistant to diet-induced obesity. J Biol Chem. 2008;283(26):18411–18421.
    View this article via: PubMed CrossRef Google Scholar
  17. Han YH, et al. RORα induces KLF4-mediated M2 polarization in the liver macrophages that protect against nonalcoholic steatohepatitis. Cell Rep. 2017;20(1):124–135.
    View this article via: PubMed CrossRef Google Scholar
  18. de Urquiza AM, et al. Docosahexaenoic acid, a ligand for the retinoid X receptor in mouse brain. Science. 2000;290(5499):2140–2144.
    View this article via: PubMed CrossRef Google Scholar
  19. Chiang N, Serhan CN. Structural elucidation and physiologic functions of specialized pro-resolving mediators and their receptors. Mol Aspects Med. 2017;58:114–129.
    View this article via: PubMed CrossRef Google Scholar
  20. Krishnamoorthy S, et al. Resolvin D1 binds human phagocytes with evidence for proresolving receptors. Proc Natl Acad Sci U S A. 2010;107(4):1660–1665.
    View this article via: PubMed CrossRef Google Scholar
  21. Colas RA, et al. Identification and actions of the maresin 1 metabolome in infectious inflammation. J Immunol. 2016;197(11):4444–4452.
    View this article via: PubMed CrossRef Google Scholar
  22. Rius B, et al. The specialized proresolving lipid mediator maresin 1 protects hepatocytes from lipotoxic and hypoxia-induced endoplasmic reticulum stress. FASEB J. 2017;31(12):5384–5398.
    View this article via: PubMed CrossRef Google Scholar
  23. Jetten AM. Retinoid-related orphan receptors (RORs): critical roles in development, immunity, circadian rhythm, and cellular metabolism. Nucl Recept Signal. 2009;7:e003.
    View this article via: PubMed Google Scholar
  24. Mamontova A, et al. Severe atherosclerosis and hypoalphalipoproteinemia in the staggerer mouse, a mutant of the nuclear receptor RORalpha. Circulation. 1998;98(24):2738–2743.
    View this article via: PubMed CrossRef Google Scholar
  25. Viola JR, et al. Resolving lipid mediators maresin 1 and resolvin D2 prevent atheroprogression in mice. Circ Res. 2016;119(9):1030–1038.
    View this article via: PubMed CrossRef Google Scholar
Version history
  • Version 1 (March 11, 2019): Electronic publication
  • Version 2 (April 1, 2019): Print issue publication

Article tools

  • View PDF
  • Download citation information
  • Send a comment
  • Terms of use
  • Standard abbreviations
  • Need help? Email the journal

Metrics

  • Article usage
  • Citations to this article

Go to

  • Top
  • Abstract
  • Inflammation and its resolution: role of maresins
  • Nonresolving inflammation in nonalcoholic steatohepatitis
  • MaR1: a novel agonist for RORα?
  • Feed-forward loop for the activation of RORα
  • Future directions
  • Acknowledgments
  • Footnotes
  • References
  • Version history
Advertisement
Advertisement

Copyright © 2025 American Society for Clinical Investigation
ISSN: 0021-9738 (print), 1558-8238 (online)

Sign up for email alerts