Issue published September 15, 2002 Previous issue | Next issue
-
Research Articles
×
Abstract
Research Article
Authors
Hairong Peng, Vonda Wright, Arvydas Usas, Brian Gearhart, Hsain-Chung Shen, James Cummins, Johnny Huard
×Abstract
Research Article
Authors
Oliver G. Opitz, Hideki Harada, Yasir Suliman, Ben Rhoades, Norman E. Sharpless, Ralph Kent, Levy Kopelovich, Hiroshi Nakagawa, Anil K. Rustgi
×Abstract
Research Article
Authors
Shoshana Yakar, Clifford J. Rosen, Wesley G. Beamer, Cheryl L. Ackert-Bicknell, Yiping Wu, Jun-Li Liu, Guck T. Ooi, Jennifer Setser, Jan Frystyk, Yves R. Boisclair, Derek LeRoith
×Abstract
Research Article
Authors
Timothy G. Butler, Jeff Schwartz, I. Caroline McMillen
×Abstract
Research Article
Authors
Peter T. Lee, Amy Putnam, Kamel Benlagha, Luc Teyton, Peter A. Gottlieb, Albert Bendelac
×Abstract
Research Article
Authors
Yoram G. Weiss, Alina Maloyan, John Tazelaar, Nichelle Raj, Clifford S. Deutschman
×Abstract
Research Article
Authors
Emanuela Gussoni, Richard R. Bennett, Kristina R. Muskiewicz, Todd Meyerrose, Jan A. Nolta, Irene Gilgoff, James Stein, Yiu-mo Chan, Hart G. Lidov, Carsten G. Bönnemann, Arpad von Moers, Glenn E. Morris, Johan T. den Dunnen, Jeffrey S. Chamberlain, Louis M. Kunkel, Kenneth Weinberg
×Abstract
Research Article
Authors
Joëlle Dupont, Jean Pierre Renou, Moshe Shani, Lothar Hennighausen, Derek LeRoith
×Abstract
Mutations in the genes encoding hepatocyte nuclear factor 4α (HNF-4α) and HNF-1α impair insulin secretion and cause maturity onset diabetes of the young (MODY). HNF-4α is known to be an essential positive regulator of HNF-1α. More recent data demonstrates that HNF-4α expression is dependent on HNF-1α in mouse pancreatic islets and exocrine cells. This effect is mediated by binding of HNF-1α to a tissue-specific promoter (P2) located 45.6 kb upstream from the previously characterized Hnf4α promoter (P1). Here we report that the expression of HNF-4α in human islets and exocrine cells is primarily mediated by the P2 promoter. Furthermore, we describe a G → A mutation in a conserved nucleotide position of the HNF-1α binding site of the P2 promoter, which cosegregates with MODY. The mutation results in decreased affinity for HNF-1α, and consequently in reduced HNF-1α–dependent activation. These findings provide genetic evidence that HNF-1α serves as an upstream regulator of HNF-4α and interacts directly with the P2 promoter in human pancreatic cells. Furthermore, they indicate that this regulation is essential to maintain normal pancreatic function.
Authors
Sara K. Hansen, Marcelina Párrizas, Maria L. Jensen, Stepanka Pruhova, Jakob Ek, Sylvia F. Boj, Anders Johansen, Miguel A. Maestro, Francisca Rivera, Hans Eiberg, Michal Andel, Jan Lebl, Oluf Pedersen, Jorge Ferrer, Torben Hansen
×Abstract
Research Article
Authors
Ganesan Ramesh, W. Brian Reeves
×Abstract
Research Article
Authors
Brendan A. Hilliard, Nicola Mason, Lingyun Xu, Jing Sun, Salah-Eddine Lamhamedi-Cherradi, Hsiou-Chi Liou, Christopher Hunter, Youhai H. Chen
×Abstract
Research Article
Authors
Kathrin Maedler, Pavel Sergeev, Frédéric Ris, José Oberholzer, Helen I. Joller-Jemelka, Giatgen A. Spinas, Nurit Kaiser, Philippe A. Halban, Marc Y. Donath
×Abstract
Research Article
Authors
Xinli Zhang, Shun’ichi Kuroda, Dale Carpenter, Ichiro Nishimura, Chia Soo, Rex Moats, Keisuke Iida, Eric Wisner, Fei-Ya Hu, Steve Miao, Steve Beanes, Catherine Dang, Heleni Vastardis, Michael Longaker, Katsuyuki Tanizawa, Norihiro Kanayama, Naoaki Saito, Kang Ting
Copyright © 2025 American Society for Clinical Investigation
ISSN: 0021-9738 (print), 1558-8238 (online)