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Craniosynostosis in transgenic mice overexpressing Nell-1
Xinli Zhang, … , Naoaki Saito, Kang Ting
Xinli Zhang, … , Naoaki Saito, Kang Ting
Published September 15, 2002
Citation Information: J Clin Invest. 2002;110(6):861-870. https://doi.org/10.1172/JCI15375.
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Article Genetics

Craniosynostosis in transgenic mice overexpressing Nell-1

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Abstract

Research Article

Authors

Xinli Zhang, Shun’ichi Kuroda, Dale Carpenter, Ichiro Nishimura, Chia Soo, Rex Moats, Keisuke Iida, Eric Wisner, Fei-Ya Hu, Steve Miao, Steve Beanes, Catherine Dang, Heleni Vastardis, Michael Longaker, Katsuyuki Tanizawa, Norihiro Kanayama, Naoaki Saito, Kang Ting

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Figure 1

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Nell-1 transgenic mice compared with nontransgenic littermates. (a) Tran...
Nell-1 transgenic mice compared with nontransgenic littermates. (a) Transgene copy number. The founders (FA and FB) and their progeny (TF2A1, TF2A2, and TF2B1) have copy numbers between 50 and 100. TF2A1 and TF2A2 are from the founder A line. TF2B1, TF2B2, and TF2B3 are from the founder B line. (b) RT-PCR analyses of Nell-1 RNA expression in both founders. C, control Nell-1 plasmid; M, muscle; H, heart; B, bone; K, kidney; L, liver. (c) Whole body (without head) RNA of newborn progeny. TF2A1 and TF2A2 express different levels of Nell-1. TF2B1 expresses Nell-1 weakly, while TF2B2 and TF2B3 have no Nell-1 expression. (d) Left panels, immunolocalization of Nell-1 protein in newborn NF2 epithelium, muscle, and calvarial bone. There is no detectable Nell-1 expression (brown staining indicates the presence of Nell-1) except some staining in the calvarial bone. Right panels, immunolocalization of Nell-1 protein in TF2A2 epithelium, muscle, and calvarial bone. Abundant Nell-1 expression is present throughout all soft tissue layers as well as in bone. Bar represents 50 μm.
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