(A and B) Representative μCT images of bone regeneration 7 days after femoral bone marrow ablation and quantitative analysis of bone parameter in regeneration area in 6-week-old Slit2^fl/fl and Slit2^th male mice. n = 5. Scale bars: 1 mm. (C) Representative flow cytometry plots 7 days after femoral bone marrow ablation and relative frequency of SSCs from femurs of 6-week-old Slit2^fl/fl and Slit2^th male mice. n = 5. (D) Representative confocal images 7 days after femoral bone marrow ablation. Shown are TH (red) and DAPI (blue) signal and quantitative analysis of TH⁺ sympathetic nerves in femur sections. Marrow ablation was conducted in 6-week-old Slit2^fl/fl and Slit2^th male mice. n = 6; Scale bars, 500 μm. (E) Representative μCT images 21 days after femur fracture and quantitative analysis of callus bone volume. Fractures were performed in 6-week-old Slit2^fl/fl and Slit2^th male mice. n = 6. Scale bars: 2 mm. (F) H&E staining visualizing the callus formed 21 days after femur fracture in 6-week-old Slit2^fl/fl and Slit2^th male mice. Scale bars: 200 μm. (G) Representative flow cytometry plots 14 days after femur fracture and the relative frequency of SSCs from the femurs of 6-week-old Slit2^fl/fl and Slit2^th male mice. n = 5 per group. (H) Representative μCT images 21 days after femur fracture and quantitative analysis of bone callus volume. Fractures were performed in 6-week-old Fstl1^fl/fl and Fstl1^Dbh-cre-ert2 male mice. n = 5. Scale bars: 2 mm. (I) Representative flow cytometry plots 14 days after femur fracture and relative frequency of SSCs from femurs of 6-week-old Fstl1^fl/fl and Fstl1^Dbh-cre-ert2 male mice. Fstl1^fl/fl, n = 6; Fstl1^Dbh-cre-ert2, n = 5. Error bars indicate mean ± SEM. *P < 0.05, **P < 0.01, by unpaired, 2-tailed Student’s t test for 2-group comparisons.