Neal B. Blatt, Jeffrey J. Bednarski, Roscoe E. Warner, Francesco Leonetti, Kathryn M. Johnson, Anthony Boitano, Raymond Yung, Bruce C. Richardson, Kent J. Johnson, Jonathan A. Ellman, Anthony W. Opipari Jr., Gary D. Glick
Mediators of Bz-423–induced apoptosis. (a) Ramos cells were incubated with vehicle or Bz-423 for 1 hour. Fluorescence intensity increased above that of control (shaded histogram) with 5 and 10 μM Bz-423 (green and red histograms, respectively). (b) Time course of changes detected in Ramos cells upon treatment with Bz-423 (10 μM). Data are presented as percent cells with indicated response relative to time-matched vehicle controls. No changes in cells treated with vehicle during this time frame were observed. The error on the DiOC6 curve is sufficiently low that the error bars are not visible. Inset: Cytochrome c release. First lane: purified cytochrome c (20 ng); second lane: cytosolic fraction isolated 5 hours after incubation with vehicle; third and fourth lanes: cytosolic fractions isolated 1 and 5 hours after incubation with Bz-423, respectively. a and b represent data from more than five separate determinations.