LID+ALSKO mice exhibit alterations in growth plate height. Representative proximal tibia sections from control (a), LID (b), ALSKO (c), and LID+ALSKO (d) mice at postnatal day 28. Sections were visualized with the Masson trichrome technique, which stains nuclei black, stains collagen blue, and stains calcified matrix red. In LID+ALSKO mice, the total height of the growth plate is significantly reduced (bar, 200 μm). Inserts (bar = 50 μm) show the germinal zone (GZ), proliferating zone (PZ), and hypertrophic zone (HZ) of chondrocytes.