Deletion of Lats1/2 in adult kidney epithelia leads to renal cell carcinoma
Phoebe Carter, … , Payal Kapur, Thomas J. Carroll
Phoebe Carter, … , Payal Kapur, Thomas J. Carroll
Published June 1, 2021
Citation Information: J Clin Invest. 2021;131(11):e144108. https://doi.org/10.1172/JCI144108.
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Research Article Cell biology Oncology

Deletion of Lats1/2 in adult kidney epithelia leads to renal cell carcinoma

Abstract

Authors

Phoebe Carter, Ulrike Schnell, Christopher Chaney, Betty Tong, Xinchao Pan, Jianhua Ye, Glenda Mernaugh, Jennifer L. Cotton, Vitaly Margulis, Junhao Mao, Roy Zent, Bret M. Evers, Payal Kapur, Thomas J. Carroll

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Figure 8

Treatment with verteporfin, a Yap inhibitor, partially rescues Lats1/2 deletion phenotype.

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Treatment with verteporfin, a Yap inhibitor, partially rescues Lats1/2 d...
(A–F) H&E-stained sections of control (A and D; n = 3) or Slc34a1CreERT2 Lats1/2c/c RosaTomato kidneys 2 weeks after tamoxifen and vehicle (B and E; n = 4) or tamoxifen and verteporfin (C and F; n = 2 survived to 14 days). D–F are high-magnification images of the sections in A–C, respectively. (G–L) Immunofluorescence staining on sections of control (G and J) or Slc34a1CreERT2 Lats1/2c/c RosaTomato kidneys 2 weeks after tamoxifen and vehicle (H and K) or tamoxifen and verteporfin (I and L). (G–I) Blue, DAPI; green, Sm22a; red, Tomato; white, LTL. (J–L) Single-channel (Sm22a) corresponding with G–I, respectively. Lats mutant lesions express both Tomato and Sm22a (H and K, arrows), and such lesions are markedly reduced in the cortexes of mutant kidneys treated with verteporfin (I and L). Scale bars: (A–C) 900 μm, (D–L) 100 μm.