Phoebe Carter, Ulrike Schnell, Christopher Chaney, Betty Tong, Xinchao Pan, Jianhua Ye, Glenda Mernaugh, Jennifer L. Cotton, Vitaly Margulis, Junhao Mao, Roy Zent, Bret M. Evers, Payal Kapur, Thomas J. Carroll
(A–H) Immunohistochemical staining against Yap (A–D) or p-Yap (E–H) on sections of control kidney (A and E; n = 3), KspCreERT2 Lats1c/c Lats2c/c kidney 1 week (B and F; n = 3) or 7 weeks after tamoxifen (C and G; n = 3), and KspCreERT2 Lats1c/c Lats2c/c lung 5 months after tamoxifen (D and H; n = 3). (I–N) Immunofluorescence staining on control (I and L) or Slc34a1CreERT2 Lats1/2c/c RosaYFP kidneys (J, K, M, and N) 1 week (J and M; n = 3 control, n = 5 mutant) or 1 month (K and N; n = 4) after tamoxifen. (I and J) Green, Sm22a; red, YFP; blue, DAPI; white, cytokeratin. (K) Green, Sm22a; red, E-cadherin; blue, DAPI; white, cytokeratin. L–N are single-channel images of Sm22a from I–K, respectively. All scale bars: 100 μm.