Serum triiodothyronine: measurements in human serum by radioimmunoassay with corroboration by gas-liquid chromatography

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Abstract

Serum triiodothyronine (T3) has been measured by radioimmunoassay and corroborated by analysis of the identical samples with a previously described gas-liquid chromatographic technique. Special features of the radioimmunoassay procedure which permit determinations in unextracted serum include the use of a T3-free serum preparation for the construction of the standard curve and of tetrachlorothyronine to inhibit binding of T3 to thyroxine-binding globulin.

Authors

Terunori Mitsuma, Noriyuki Nihei, Marvin C. Gershengorn, Charles S. Hollander