(A and B) BMDC^WT and DC^Anxa1–/– were infected with BCG-OVA (MOI ~2). After 4 hours of infection, CFSE-labeled purified OT-I CD8⁺ T cells were added to the culture. At day 3, CD8⁺ T cell proliferation was evaluated using CFSE dilution. Controls were CFSE-labeled OT-I CD8⁺ T cells cultured with DC without BCG-OVA infection. Each symbol represents 1 replicate (B). Results are representative of 6 independent experiments. *P < 0.05 (t test). (C and D) CFSE-labeled, purified OT-I CD8⁺ T cells were adoptively transferred to WT mice (i.v.). The following day, BCG-OVA–infected DC^WT and DC^Anxa1–/– were transferred into the footpads of WT mice (n = 3/group). (D) Percentage of proliferation of SIINFEKL-specific CD8⁺ T cells (CFSE^lo). *P < 0.05 (t test, 2 tailed). (E–H) CFSE-labeled OT-I CD8⁺ T cells were adoptively transferred (i.v.) into WT or Anxa1^–/– mice (n = 4/group). The following day, mice were injected (i.v.) with WT apoptotic cells. At day 3, T cell proliferation (E and F), frequency (G), and total number (H) of SIINFEKL-specific CD8⁺ T cells were assessed in spleens. *P < 0.05; **P < 0.01 (t test). (I–K) CFSE-labeled OT-I CD8⁺ T cells were adoptively transferred (i.v.) to WT or Anxa1^–/– mice. The following day, mice were injected (i.v.) with WT vesicles (n = 3/group). At day 4, T cell proliferation (I), frequency, (J) and total number (K) of SIINFEKL-specific CD8⁺ T cells were evaluated in popLN. *P < 0.05 (t test). Numbers above outlined areas (A and I) indicate the percentage of T cell proliferation (CFSE^lo). Numbers above bracketed lines (E) indicate the percentage of cells that had undergone 3 or more divisions (left) or fewer than 3 divisions (right). Numbers above outlined areas (G and J) indicate the percentage of CD8⁺ T cells stained with H-2K^b–SIINFEKL.