Published February 1, 2013 - More info
The CBX7 gene encodes a polycomb group protein that is known to be downregulated in many types of human cancers, although the role of this protein in carcinogenesis remains unclear. To shed light on this issue, we generated mice null for Cbx7. Mouse embryonic fibroblasts derived from these mice had a higher growth rate and reduced susceptibility to senescence compared with their WT counterparts. This was associated with upregulated expression of multiple cell cycle components, including cyclin E, which is known to play a key role in lung carcinogenesis in humans. Adult Cbx7-KO mice developed liver and lung adenomas and carcinomas. In in vivo and in vitro experiments, we demonstrated that CBX7 bound to the CCNE1 promoter in a complex that included HDAC2 and negatively regulated CCNE1 expression. Finally, we found that the lack of CBX7 protein expression in human lung carcinomas correlated with CCNE1 overexpression. These data suggest that CBX7 is a tumor suppressor and that its loss plays a key role in the pathogenesis of cancer.
Floriana Forzati, Antonella Federico, Pierlorenzo Pallante, Adele Abbate, Francesco Esposito, Umberto Malapelle, Romina Sepe, Giuseppe Palma, Giancarlo Troncone, Marzia Scarfò, Claudio Arra, Monica Fedele, Alfredo Fusco
Original citation: J. Clin. Invest. 2012;122(2):612–623. doi:10.1172/JCI58620.
Citation for this corrigendum: J. Clin. Invest. 2013;123(2):934. doi:10.1172/JCI68754.
During assembly of the figure panels for this manuscript, some bands within the Figure 1C Northern blot were inadvertently duplicated, and lanes of the Figure 4A Western blot were inappropriately removed. However, these findings were confirmed in subsequent experiments. Alternative versions of those figure panels are below.
The authors regret the error.