Go to JCI Insight
  • About
  • Editors
  • Consulting Editors
  • For authors
  • Alerts
  • Advertising/recruitment
  • Subscribe
  • Contact
  • Current Issue
  • Past Issues
  • By specialty
    • COVID-19
    • Cardiology
    • Gastroenterology
    • Immunology
    • Metabolism
    • Nephrology
    • Neuroscience
    • Oncology
    • Pulmonology
    • Vascular biology
    • All ...
  • Videos
    • Conversations with Giants in Medicine
    • Author's Takes
  • Reviews
    • View all reviews ...
    • 100th Anniversary of Insulin's Discovery (Jan 2021)
    • Hypoxia-inducible factors in disease pathophysiology and therapeutics (Oct 2020)
    • Latency in Infectious Disease (Jul 2020)
    • Immunotherapy in Hematological Cancers (Apr 2020)
    • Big Data's Future in Medicine (Feb 2020)
    • Mechanisms Underlying the Metabolic Syndrome (Oct 2019)
    • Reparative Immunology (Jul 2019)
    • View all review series ...
  • Viewpoint
  • Collections
    • Recently published
    • In-Press Preview
    • Commentaries
    • Concise Communication
    • Editorials
    • Viewpoint
    • Top read articles
  • Clinical Medicine
  • JCI This Month
    • Current issue
    • Past issues

  • Current issue
  • Past issues
  • Specialties
  • Reviews
  • Review series
  • Conversations with Giants in Medicine
  • Author's Takes
  • Recently published
  • In-Press Preview
  • Commentaries
  • Concise Communication
  • Editorials
  • Viewpoint
  • Top read articles
  • About
  • Editors
  • Consulting Editors
  • For authors
  • Alerts
  • Advertising/recruitment
  • Subscribe
  • Contact
Rap1b is required for normal platelet function and hemostasis in mice
Magdalena Chrzanowska-Wodnicka, … , Thomas H. Fischer, Gilbert C. White II
Magdalena Chrzanowska-Wodnicka, … , Thomas H. Fischer, Gilbert C. White II
Published August 1, 2005
Citation Information: J Clin Invest. 2005;115(8):2296-2296. https://doi.org/10.1172/JCI22973C1.
View: Text | PDF | Amended Article
Corrigendum Hematology

Rap1b is required for normal platelet function and hemostasis in mice

  • Text
  • PDF
Abstract

Authors

Magdalena Chrzanowska-Wodnicka, Susan S. Smyth, Simone M. Schoenwaelder, Thomas H. Fischer, Gilbert C. White II

×

Figure 1

Options: View larger image (or click on image) Download as PowerPoint
Targeted inactivation of the rap1b gene. (A) The
                    mur...
Targeted inactivation of the rap1b gene. (A) The murine rap1b gene consists of 6 coding (bands) and 1 untranslated exon (3′ UTR, open box). (B) The targeting vector contains 7.8 kb of genomic DNA flanking the neomycin-resistance cassette (neo). TK, thymidine kinase. (C) After homologous recombination, the neo cassette replaces the complete coding sequence of the rap1b gene. (D) Southern blot analysis of mouse tail DNA from heterozygous intercrosses digested with SspI and KpnI using a probe (P) that detects 5.5-kb and 9-kb fragments in the wild-type and knockout allele, respectively. (E) Western blot analysis of protein expression in platelets of indicated genotype. (F) Morphology of E15.5 wild-type (+/+) and Rap1b-null (–/–) embryos. Scale bar: 1 mm. E, EcoRI; H, HinDIII; K, KpnI; S, SspI.
Follow JCI:
Copyright © 2021 American Society for Clinical Investigation
ISSN: 0021-9738 (print), 1558-8238 (online)

Sign up for email alerts