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Leukocyte engagement of fibrin(ogen) via the integrin receptor αMβ2/Mac-1 is critical for host inflammatory response in vivo
Matthew J. Flick, … , Edward F. Plow, Jay L. Degen
Matthew J. Flick, … , Edward F. Plow, Jay L. Degen
Published June 1, 2004
Citation Information: J Clin Invest. 2004;113(11):1596-1606. https://doi.org/10.1172/JCI20741.
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Article Hematology

Leukocyte engagement of fibrin(ogen) via the integrin receptor αMβ2/Mac-1 is critical for host inflammatory response in vivo

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Abstract

The leukocyte integrin αMβ2/Mac-1 appears to support the inflammatory response through multiple ligands, but local engagement of fibrin(ogen) may be particularly important for leukocyte function. To define the biological significance of fibrin(ogen)-αMβ2 interaction in vivo, gene-targeted mice were generated in which the αMβ2-binding motif within the fibrinogen γ chain (N390RLSIGE396) was converted to a series of alanine residues. Mice carrying the Fibγ390–396A allele maintained normal levels of fibrinogen, retained normal clotting function, supported platelet aggregation, and never developed spontaneous hemorrhagic events. However, the mutant fibrinogen failed to support αMβ2-mediated adhesion of primary neutrophils, macrophages, and αMβ2-expressing cell lines. The elimination of the αMβ2-binding motif on fibrin(ogen) severely compromised the inflammatory response in vivo as evidenced by a dramatic impediment in leukocyte clearance of Staphylococcus aureus inoculated into the peritoneal cavity. This defect in bacterial clearance was due not to diminished leukocyte trafficking but rather to a failure to fully implement antimicrobial functions. These studies definitively demonstrate that fibrin(ogen) is a physiologically relevant ligand for αMβ2, integrin engagement of fibrin(ogen) is critical to leukocyte function and innate immunity in vivo, and the biological importance of fibrinogen in regulating the inflammatory response can be appreciated outside of any alteration in clotting function.

Authors

Matthew J. Flick, XinLi Du, David P. Witte, Markéta Jiroušková, Dmitry A. Soloviev, Steven J. Busuttil, Edward F. Plow, Jay L. Degen

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Figure 4

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Fibrinogen γ390–396A does not support
                            αMβ2-d...
Fibrinogen γ390–396A does not support αMβ2-dependent adhesion of primary neutrophils. Primary human neutrophils (106 cells/ml) were transferred to either uncoated wells, wells coated with 10 ∝g/ml WT mouse fibrinogen (A), wells coated with 10 ∝g/ml fibrinogen γ390–396A (B), or wells coated with 10 ∝g/ml fibrinogen γØ5 (C). Shown are representative views of bound cells after 25 minutes. (D) Quantitative comparison of neutrophil adhesion to WT, fibrinogen γ390–396A, and fibrinogen γØ5. The specificity of neutrophil engagement was established by preincubating neutrophils with 20 ∝g/ml of the rat anti-mouse αM I-domain Ab, M1/70, or control rat anti-mouse IgG for 15 minutes at room temperature before addition to the fibrinogen-coated wells. The data shown are means ± SD. HPF, high power field; Con, control.

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ISSN: 0021-9738 (print), 1558-8238 (online)

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