Crystal deposition triggers tubule dilation that accelerates cystogenesis in polycystic kidney disease

Original citation: J Clin Invest. 2019;129(10):4506-4522. https://doi.org/10.1172/JCI128503

Citation for this corrigendum: J Clin Invest. 2025;135(9):e193824. https://doi.org/10.1172/JCI193824

The dosing of a drug in the Methods and figure legends was incorrect. The corrected sentences are below.

A high dose of NaOx (70 mg/kg) was initially used to induce acute, severe nephrolithiasis in the mice, which led to rapid, abundant crystal deposition that appeared to be exclusively luminal (Figure 2, A and B).

WT male and female C57BL/6 mice (Charles River Laboratories), 8–10 weeks of age and weighing between 20 and 30 g, were challenged with a single i.p. injection of 0.22 M NaOx in 0.9% saline, sterile filtered and administered at 30 mg/kg (low dose), and euthanized at 6 hours (n = 4), 1 day (n = 12), 3 days (n = 8), and 7 days (n = 10); or at 70 mg/kg (high dose), and animals were euthanized at 3 hours (n = 4) and 1 day (n = 9) to induce acute CaOx crystal deposition.

Eight-week-old Sprague Dawley rats weighing between 200 and 300 g (Charles River Laboratories) were challenged with a single i.p. injection of 0.22 M NaOx in 0.9% saline, sterile filtered and administered at 70 mg/kg and were analyzed after 6 hours (n = 5), 1 day (n = 5), 3 days (n = 5), or 7 days (n = 3).

To induce moderate, reversible crystal deposition, we next administered a low dose of NaOx (30 mg/kg), which resulted in CaOx crystal deposition as early as 6 hours after injection, peaking at 24 hours, with excretion of most crystals by day 3 (Figure 2D).

Figure 2. (A) H&E-stained kidney sections 1 day after administration of 70 mg/kg NaOx, visualized by normal and polarized light microscopy.

Figure 2. (C) Immunoblot of total kidney lysates for p-S6 (Ser235/236), p-STAT3 (Tyr705), and total proteins 3 hours (n = 4) and 1 day (n = 9) after 70 mg/kg NaOx treatment.

Figure 2. (D) Polarized light micrographs of kidney sections from mice treated with 30 mg/kg NaOX, 6 hours (n = 4), 1 day (n = 12), 3 days (n = 8), and 7 days (n = 13) after treatment.

Figure 2. (E) Immunofluorescence staining of p-S6 (Ser235/236) and p-STAT3 (Tyr705) in mice treated with 30 mg/kg NaOx. Images of animals treated with 30 mg/kg NaOx are representative of 5 experiments.

Figure 3. (A) Polarized microscopic images of H&E-stained renal sections from rats treated with 70 mg/kg NaOx, 6 hours (n = 5), 24 hours (n = 5), 3 days (n = 5), and 7 days (n = 3) after treatment.

The authors regret the errors.

Footnotes

See the related article at Crystal deposition triggers tubule dilation that accelerates cystogenesis in polycystic kidney disease.