Meflin confers antifibrotic properties to intestinal fibroblasts in inflammatory bowel disease
Jingxi Mu, Keiko Maeda, Tadashi Iida, Shinji Mii, Nobutoshi Esaki, Yukihiro Shiraki, Yasuyuki Mizutani, Masanao Nakamura, Takeshi Yamamura, Tsunaki Sawada, Eri Ishikawa, Kentaro Murate, Takashi Hirose, Kazuhiro Furukawa, Akina Oishi, Haruhiko Suzuki, Takayoshi Kishida, Goro Nakayama, Mitsuhiro Fujishiro, Hiroki Kawashima, Atsushi Enomoto
Jingxi Mu, Keiko Maeda, Tadashi Iida, Shinji Mii, Nobutoshi Esaki, Yukihiro Shiraki, Yasuyuki Mizutani, Masanao Nakamura, Takeshi Yamamura, Tsunaki Sawada, Eri Ishikawa, Kentaro Murate, Takashi Hirose, Kazuhiro Furukawa, Akina Oishi, Haruhiko Suzuki, Takayoshi Kishida, Goro Nakayama, Mitsuhiro Fujishiro, Hiroki Kawashima, Atsushi Enomoto
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Research Article Gastroenterology Inflammation

Meflin confers antifibrotic properties to intestinal fibroblasts in inflammatory bowel disease

Abstract

Dysfunctional intestinal fibrosis is an irreversible complication of Crohn’s disease (CD). The complex heterogeneity of intestinal mesenchymal cells makes it difficult to understand the pathogenesis of intestinal fibrosis. Previously, we identified Meflin as a marker of fibroblast subsets. This study aimed to explore the role of Meflin-positive fibroblasts in intestinal fibrogenesis and investigate the potential of pharmacological control of Meflin expression as a treatment for patients with CD. Our results indicated that Meflin expression was upregulated in fibroblasts at the early stage of fibrosis but was downregulated in established fibrosis in both patients with CD and 2 different mouse models, which are the chronic dextran sodium sulfate (DSS) model and an IL-10–deficient model that spontaneously develops intestinal inflammation. Meflin-deficient mice exacerbated intestinal fibrosis with dysregulated expression of noncanonical Wnt ligand WNT5A and its receptor ROR2. Pharmacologically induced Meflin expression through the administration of a synthetic retinoid reversed intestinal fibrosis in the DSS model and suppressed profibrotic protein secretion in fibroblasts isolated from patients with CD. Our findings indicate that Meflin-positive fibroblasts represent a functional subpopulation that suppresses intestinal fibrosis. Augmentation of Meflin expression shows antifibrotic effects and holds promise as a therapeutic approach for intestinal fibrosis in patients with CD.

Authors

Jingxi Mu, Keiko Maeda, Tadashi Iida, Shinji Mii, Nobutoshi Esaki, Yukihiro Shiraki, Yasuyuki Mizutani, Masanao Nakamura, Takeshi Yamamura, Tsunaki Sawada, Eri Ishikawa, Kentaro Murate, Takashi Hirose, Kazuhiro Furukawa, Akina Oishi, Haruhiko Suzuki, Takayoshi Kishida, Goro Nakayama, Mitsuhiro Fujishiro, Hiroki Kawashima, Atsushi Enomoto

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Figure 6

Am80-induced reversion of established intestinal fibrosis is mediated by Meflin expression.

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Am80-induced reversion of established intestinal fibrosis is mediated by...
(A) Schematic diagram showing the experimental setup. (B and C) Body weight changes over time, and colon length at Day 63 is measured, followed by quantification. (D–F) Colon tissue sections from Meflin-KO mice after Am80 administration are stained with H&E (D), IHC for α-SMA (E), and Sirius Red (F), followed by collagen layer thickness, quantification of Sirius Red+ areas, and fibrosis score. (E and F) (n = 5 and 4 for the control and Am80 groups, respectively). (G) Am80 (3 mg/kg/day) or DMSO is orally administered every day to Meflin-KO mice from weeks 10–13 after the 3 cycles of DSS administration. (H and I) Body weight changes over time, and colon length at Day 92 is evaluated. (J–L) Representative images for H&E, IHC, for α-SMA, and Sirius Red staining performed in colon tissues from Meflin-KO mice after DSS and subsequent Am80 administrations (n = 4 and 6 for the control and Am80 groups, respectively), followed by quantification. (B right panel, C, H right panel, I) Each dot represents an individual sample. (E, F, K, L) 5 HPFs per area were quantified for each mouse. Small gray dots indicate individual HPFs, and black triangles indicate mouse-level means used for statistical analysis. Scale bars: 40 μm. Statistical significance was determined using 2-tailed Student’s t tests.