Fate-mapping infiltrating monocytes following experimental myocardial infarction reveals differentiation trajectories in the infarcted heart

Andrew L. Koenig; Farid F. Kadyrov; Junedh M. Amrute; Steven Yang; Carla J. Weinheimer; Jessica M. Nigro; Attila Kovacs; Wenjun Li; Gabriella B. Smith; Lance Yeh; Daniel Kreisel; Kory J. Lavine

doi:10.1172/JCI189684

Fate-mapping infiltrating monocytes following experimental myocardial infarction revealsdifferentiation trajectories in the infarcted heart

Andrew L. Koenig,¹ Farid F. Kadyrov,¹ Junedh M. Amrute,¹ Steven Yang,¹ Carla J. Weinheimer,¹ Jessica M. Nigro,¹ Attila Kovacs,¹ Wenjun Li,² Gabriella B. Smith,¹ Lance Yeh,¹ Daniel Kreisel,² and Kory J. Lavine¹

Published July 7, 2026 - More info

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Abstract

Inflammation contributes to the pathogenesis of myocardial infarction and heart failure and represents a viable therapeutic target. Monocytes and their progeny are highly abundant and display striking functional diversity, serving as key determinants of myocardial inflammation and tissue repair. Much remains to be learned regarding mechanisms and signaling events that instruct monocyte fate decisions. We devised a genetic lineage tracing strategy using Ccr2^crERT2Rosa26^LSL-tdTomato mice in combination with single cell RNA-sequencing to map the differentiation trajectories of monocytes that infiltrate the heart after reperfused myocardial infarction. Monocytes were recruited to the heart early after injury and gave rise to transcriptionally distinct and spatially restricted macrophage and dendritic cell-like subsets that were specified prior to extravasation and chronically persisted within the myocardium. Pseudotime analysis predicted two differentiation trajectories of monocyte-derived macrophages that are partitioned into the border and infarct zones, respectively. Among these trajectories, we demonstrated that macrophages expressing a type I interferon responsive signature were an intermediate population that gave rise to MHC-II^hi macrophages, were localized within the border zone, induce regulatory T cells, and promote myocardial protection. Collectively, these data uncover complexities of monocyte differentiation in the infarcted heart and suggest that modulating monocyte fate decisions may have clinical implications.

Fate-mapping infiltrating monocytes following experimental myocardial infarction revealsdifferentiation trajectories in the infarcted heart

Andrew L. Koenig,¹ Farid F. Kadyrov,¹ Junedh M. Amrute,¹ Steven Yang,¹ Carla J. Weinheimer,¹ Jessica M. Nigro,¹ Attila Kovacs,¹ Wenjun Li,² Gabriella B. Smith,¹ Lance Yeh,¹ Daniel Kreisel,² and Kory J. Lavine¹

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Fate-mapping infiltrating monocytes following experimental myocardial infarction revealsdifferentiation trajectories in the infarcted heart

Andrew L. Koenig,1 Farid F. Kadyrov,1 Junedh M. Amrute,1 Steven Yang,1 Carla J. Weinheimer,1 Jessica M. Nigro,1 Attila Kovacs,1 Wenjun Li,2 Gabriella B. Smith,1 Lance Yeh,1 Daniel Kreisel,2 and Kory J. Lavine1

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Andrew L. Koenig,¹ Farid F. Kadyrov,¹ Junedh M. Amrute,¹ Steven Yang,¹ Carla J. Weinheimer,¹ Jessica M. Nigro,¹ Attila Kovacs,¹ Wenjun Li,² Gabriella B. Smith,¹ Lance Yeh,¹ Daniel Kreisel,² and Kory J. Lavine¹