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Open Access | 10.1172/JCI169753
1Heart and Vascular Center, Semmelweis University, Budapest, Hungary
2Department of Biophysics and Radiation Biology, Semmelweis University, Budapest, Hungary
3Medical School, University of Heidelberg, Heidelberg, Germany
41st Department of Pathology and Experimental Cancer Research, Semmelweis University, Budapest, Hungary
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1Heart and Vascular Center, Semmelweis University, Budapest, Hungary
2Department of Biophysics and Radiation Biology, Semmelweis University, Budapest, Hungary
3Medical School, University of Heidelberg, Heidelberg, Germany
41st Department of Pathology and Experimental Cancer Research, Semmelweis University, Budapest, Hungary
Find articles by Tordai, H. in: JCI | PubMed | Google Scholar
1Heart and Vascular Center, Semmelweis University, Budapest, Hungary
2Department of Biophysics and Radiation Biology, Semmelweis University, Budapest, Hungary
3Medical School, University of Heidelberg, Heidelberg, Germany
41st Department of Pathology and Experimental Cancer Research, Semmelweis University, Budapest, Hungary
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Kiss, B.
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1Heart and Vascular Center, Semmelweis University, Budapest, Hungary
2Department of Biophysics and Radiation Biology, Semmelweis University, Budapest, Hungary
3Medical School, University of Heidelberg, Heidelberg, Germany
41st Department of Pathology and Experimental Cancer Research, Semmelweis University, Budapest, Hungary
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Török, G.
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1Heart and Vascular Center, Semmelweis University, Budapest, Hungary
2Department of Biophysics and Radiation Biology, Semmelweis University, Budapest, Hungary
3Medical School, University of Heidelberg, Heidelberg, Germany
41st Department of Pathology and Experimental Cancer Research, Semmelweis University, Budapest, Hungary
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1Heart and Vascular Center, Semmelweis University, Budapest, Hungary
2Department of Biophysics and Radiation Biology, Semmelweis University, Budapest, Hungary
3Medical School, University of Heidelberg, Heidelberg, Germany
41st Department of Pathology and Experimental Cancer Research, Semmelweis University, Budapest, Hungary
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1Heart and Vascular Center, Semmelweis University, Budapest, Hungary
2Department of Biophysics and Radiation Biology, Semmelweis University, Budapest, Hungary
3Medical School, University of Heidelberg, Heidelberg, Germany
41st Department of Pathology and Experimental Cancer Research, Semmelweis University, Budapest, Hungary
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1Heart and Vascular Center, Semmelweis University, Budapest, Hungary
2Department of Biophysics and Radiation Biology, Semmelweis University, Budapest, Hungary
3Medical School, University of Heidelberg, Heidelberg, Germany
41st Department of Pathology and Experimental Cancer Research, Semmelweis University, Budapest, Hungary
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1Heart and Vascular Center, Semmelweis University, Budapest, Hungary
2Department of Biophysics and Radiation Biology, Semmelweis University, Budapest, Hungary
3Medical School, University of Heidelberg, Heidelberg, Germany
41st Department of Pathology and Experimental Cancer Research, Semmelweis University, Budapest, Hungary
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1Heart and Vascular Center, Semmelweis University, Budapest, Hungary
2Department of Biophysics and Radiation Biology, Semmelweis University, Budapest, Hungary
3Medical School, University of Heidelberg, Heidelberg, Germany
41st Department of Pathology and Experimental Cancer Research, Semmelweis University, Budapest, Hungary
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3Medical School, University of Heidelberg, Heidelberg, Germany
41st Department of Pathology and Experimental Cancer Research, Semmelweis University, Budapest, Hungary
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1Heart and Vascular Center, Semmelweis University, Budapest, Hungary
2Department of Biophysics and Radiation Biology, Semmelweis University, Budapest, Hungary
3Medical School, University of Heidelberg, Heidelberg, Germany
41st Department of Pathology and Experimental Cancer Research, Semmelweis University, Budapest, Hungary
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Bedics, G.
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1Heart and Vascular Center, Semmelweis University, Budapest, Hungary
2Department of Biophysics and Radiation Biology, Semmelweis University, Budapest, Hungary
3Medical School, University of Heidelberg, Heidelberg, Germany
41st Department of Pathology and Experimental Cancer Research, Semmelweis University, Budapest, Hungary
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Bödör, C.
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1Heart and Vascular Center, Semmelweis University, Budapest, Hungary
2Department of Biophysics and Radiation Biology, Semmelweis University, Budapest, Hungary
3Medical School, University of Heidelberg, Heidelberg, Germany
41st Department of Pathology and Experimental Cancer Research, Semmelweis University, Budapest, Hungary
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1Heart and Vascular Center, Semmelweis University, Budapest, Hungary
2Department of Biophysics and Radiation Biology, Semmelweis University, Budapest, Hungary
3Medical School, University of Heidelberg, Heidelberg, Germany
41st Department of Pathology and Experimental Cancer Research, Semmelweis University, Budapest, Hungary
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1Heart and Vascular Center, Semmelweis University, Budapest, Hungary
2Department of Biophysics and Radiation Biology, Semmelweis University, Budapest, Hungary
3Medical School, University of Heidelberg, Heidelberg, Germany
41st Department of Pathology and Experimental Cancer Research, Semmelweis University, Budapest, Hungary
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Published November 14, 2023 - More info
Heterozygous (HET) truncating mutations in the TTN gene (TTNtv) encoding the giant titin protein are the most common genetic cause of dilated cardiomyopathy (DCM). However, the molecular mechanisms by which TTNtv mutations induce DCM are controversial. Here we investigated 127 clinically identified DCM human cardiac samples with next-generation sequencing (NGS), high-resolution gel electrophoresis, Western blot analysis and super-resolution microscopy in order to dissect the structural and functional consequences of TTNtv mutations. The occurrence of TTNtv was found to be 15% in the DCM cohort. Truncated titin proteins matching, by molecular weight, the gene-sequence predictions were detected in the majority of the TTNtv+ samples. Full length titin was reduced in TTNtv+ compared to TTNtv- samples. Proteomic analysis of washed myofibrils and Stimulated Emission Depletion (STED) super-resolution microscopy of myocardial sarcomeres labeled with sequence-specific anti-titin antibodies revealed that truncated titin is structurally integrated in the sarcomere. Sarcomere length-dependent anti-titin epitope position, shape and intensity analyses pointed at possible structural defects in the I/A junction and the M-band of TTNtv+ sarcomeres, which likely contribute, possibly via faulty mechanosensor function, to the development of manifest DCM.