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Erratum Open Access | 10.1172/JCI161565

Innate and adaptive nasal mucosal immune responses following experimental human pneumococcal colonization

Simon P. Jochems, Karin de Ruiter, Carla Solórzano, Astrid Voskamp, Elena Mitsi, Elissavet Nikolaou, Beatriz F. Carniel, Sherin Pojar, Esther L. German, Jesús Reiné, Alessandra Soares-Schanoski, Helen Hill, Rachel Robinson, Angela D. Hyder-Wright, Caroline M. Weight, Pascal F. Durrenberger, Robert S. Heyderman, Stephen B. Gordon, Hermelijn H. Smits, Britta C. Urban, Jamie Rylance, Andrea M. Collins, Mark D. Wilkie, Lepa Lazarova, Samuel C. Leong, Maria Yazdanbakhsh, and Daniela M. Ferreira

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Published June 1, 2022 - More info

Published in Volume 132, Issue 11 on June 1, 2022
J Clin Invest. 2022;132(11):e161565. https://doi.org/10.1172/JCI161565.
© 2022 Jochems et al. This work is licensed under the Creative Commons Attribution 4.0 International License. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
Published June 1, 2022 - Version history
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Innate and adaptive nasal mucosal immune responses following experimental human pneumococcal colonization
Simon P. Jochems, … , Maria Yazdanbakhsh, Daniela M. Ferreira
Simon P. Jochems, … , Maria Yazdanbakhsh, Daniela M. Ferreira
Research Article Immunology Infectious disease

Innate and adaptive nasal mucosal immune responses following experimental human pneumococcal colonization

Abstract

Streptococcus pneumoniae (Spn) is a common cause of respiratory infection, but also frequently colonizes the nasopharynx in the absence of disease. We used mass cytometry to study immune cells from nasal biopsy samples collected following experimental human pneumococcal challenge in order to identify immunological mechanisms of control of Spn colonization. Using 37 markers, we characterized 293 nasal immune cell clusters, of which 7 were associated with Spn colonization. B cell and CD161+CD8+ T cell clusters were significantly lower in colonized than in noncolonized subjects. By following a second cohort before and after pneumococcal challenge we observed that B cells were depleted from the nasal mucosa upon Spn colonization. This associated with an expansion of Spn polysaccharide–specific and total plasmablasts in blood. Moreover, increased responses of blood mucosa-associated invariant T (MAIT) cells against in vitro stimulation with pneumococcus prior to challenge associated with protection against establishment of Spn colonization and with increased mucosal MAIT cell populations. These results implicate MAIT cells in the protection against pneumococcal colonization and demonstrate that colonization affects mucosal and circulating B cell populations.

Authors

Simon P. Jochems, Karin de Ruiter, Carla Solórzano, Astrid Voskamp, Elena Mitsi, Elissavet Nikolaou, Beatriz F. Carniel, Sherin Pojar, Esther L. German, Jesús Reiné, Alessandra Soares-Schanoski, Helen Hill, Rachel Robinson, Angela D. Hyder-Wright, Caroline M. Weight, Pascal F. Durrenberger, Robert S. Heyderman, Stephen B. Gordon, Hermelijn H. Smits, Britta C. Urban, Jamie Rylance, Andrea M. Collins, Mark D. Wilkie, Lepa Lazarova, Samuel C. Leong, Maria Yazdanbakhsh, Daniela M. Ferreira

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Original citation: J Clin Invest. 2019;129(10):4523–4538. https://doi.org/10.1172/JCI128865

Citation for this erratum: J Clin Invest. 2022;132(11):e161565. https://doi.org/10.1172/JCI161565

During the preparation of this manuscript, the labels for Figure 5, A and D, for Streptococcus pneumoniae (Spn) carriage– (Spn–) and carriage+ (Spn+) were inverted. The correct figure panels are below. The HTML and PDF versions of the figure have also been updated.

The JCI regrets the errors.

Footnotes

See the related article at Innate and adaptive nasal mucosal immune responses following experimental human pneumococcal colonization.

Version history
  • Version 1 (June 1, 2022): Electronic publication
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