Intracellular ROM accumulation in neutrophils from nonpregnant (a) and pregnant (b) women. Cells were labeled with H₂TMRos. Cells were treated with buffer alone (–LPS), LPS, or LPS plus IFN-γ. The cells were quantitatively analyzed for fluorescence intensity using emission spectroscopy. ROM accumulation in cells from nonpregnant women is greatly enhanced by LPS and IFN-γ (a). However, no such enhancement is seen for neutrophils from pregnant women (b). Importantly, unstimulated neutrophils from pregnant women have amounts of internal ROMs similar to those of LPS-stimulated cells from nonpregnant women. n ≥ 7.