Molecular mechanisms of blister formation in bullous impetigo and staphylococcal scalded skin syndrome
Yasushi Hanakawa, … , Masayuki Amagai, John R. Stanley
Yasushi Hanakawa, … , Masayuki Amagai, John R. Stanley
Published July 1, 2002
Citation Information: J Clin Invest. 2002;110(1):53-60. https://doi.org/10.1172/JCI15766.
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Article Infectious disease

Molecular mechanisms of blister formation in bullous impetigo and staphylococcal scalded skin syndrome

Abstract

Research Article

Authors

Yasushi Hanakawa, Norman M. Schechter, Chenyan Lin, Luis Garza, Hong Li, Takayuki Yamaguchi, Yasuyuki Fudaba, Koji Nishifuji, Motoyuki Sugai, Masayuki Amagai, John R. Stanley

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Point mutation of serine 195 (chymotrypsin numbering), the presumed cata...
Point mutation of serine 195 (chymotrypsin numbering), the presumed catalytically active serine, of ETs inhibits cleavage of Dsg1. (a) Anti–E-tag antibody immunoblot of SDS-PAGE of hDsg1E incubated with RN4220 staphylococcal vector supernatant (RN), wild-type (WT) ETA, ETA Cmu (serine 195 mutated to cysteine), and ETA Amu (serine 195 mutated to alanine) shows markedly decreased cleavage with ETA Cmu compared with wild-type ETA. ETA Amu shows no catalytic activity. Horizontal lines indicate migration of molecular weight markers of 83 kDa (top) and 32 kDa. (b) Anti–FLAG-tag immunoblots of anti–FLAG-tag immunoprecipitates of extracts of mDsg1-FLAG adenovirus–transduced cells that were incubated with ETB or ETB Amu. ETB Amu shows no cleavage. Horizontal lines, from top, indicate migration of molecular weight markers of 203 kDa, 115 kDa, and 93 kDa. (c) Anti–E-tag antibody immunoblot of SDS-PAGE of hDsg1E incubated with ETD and ETD Amu. ETD Amu does not cleave hDsg1. Horizontal lines, from top, indicate migration of molecular weight markers of 83 kDa and 34 kDa. Uncleaved Dsg1 (open arrowhead) and its carboxy-terminal cleavage product (filled arrowhead) are indicated.