Pretreatment of TIMP-1 DNA inhibits recombinant VEGF-induced angiogenesis. Groups of mice were treated intraocularly with TIMP-1 DNA or vector DNA, and hydron pellets containing rhVEGF (90 ng) were implanted into the corneal pockets. The total number of neovessels originating in the limbus and the area of neovascularization were calculated. Data are compiled from two separate experiments (five mice per group) with similar results. P < 0.05.