(A–C) Seven-week-old WT male C57BL/6 mice were fed with HFD for 12 weeks. After 10 weeks of diet feeding, DHA was i.p. injected daily at dose of 5 mg/kg BW with vehicle or 5 mg/kg BW baicalein for 2 weeks. (A) Representative captured liver tissues and H&E staining of liver sections. Scale bar: 200 μm. (B) Amount of MaR1 in the liver tissues were analyzed. (C) The liver macrophages were isolated from mice and the CD206⁺/CD80⁺ ratio of F4/80⁺ cells was determined by flow cytometry. **P < 0.01; ^##P < 0.01 (n = 6–8) for B and C. (D–G) Seven-week-old WT male C57BL/6 mice were fed with HFD for 16 weeks. After 13 weeks of diet feeding, an intravenous injection of either AAV-GFP or AAV–12-LOX at 5 × 10⁹ virus genomes was conducted. DHA was i.p. injected daily at doses 1 mg/kg BW for 3 weeks after virus injection. (D) Representative captured liver tissues and the liver weights at the end of experiments. (E) Sirius red staining of liver sections. Fibrotic area in the liver sections was analyzed by Image J. Scale bar: 50 μm. (F) Levels of α-SMA, TGF-β, and 12-LOX proteins in the liver tissues were analyzed by Western blotting. (G) The amount of MaR1 in liver tissues were measured. *P < 0.05, ^#P < 0.05, and ^##P < 0.01 (n = 5) for D–G. (H) Isolated liver macrophages were treated with 50 μM DHA and then infected by AAV-GFP or AAV–12-LOX. The CD206⁺/CD80⁺ ratio of F4/80⁺ cells was determined. *P < 0.05, ^#P < 0.05 (n = 3). The data represent mean ± SD. Data were analyzed by Mann–Whitney U test for simple comparisons or Kruskal-Wallis test for multiple groups.