Advertisement

Commentary Free access | 10.1172/JCI121053

Lymph node fibrosis: a structural barrier to unleashing effective vaccine immunity

Boris Julg and Galit Alter

Ragon Institute of MGH, MIT and Harvard, Cambridge, Massachusetts, USA. Infectious Disease Unit, Massachusetts General Hospital, Boston, Massachusetts, USA.

Address correspondence to: Galit Alter, Ragon Institute of MGH, MIT and Harvard, 400 Technology Square, Cambridge, Massachusetts 02139, USA. Phone: 617.724.0546; Email: galter@mgh.harvard.edu.

Find articles by Julg, B. in: JCI | PubMed | Google Scholar

Ragon Institute of MGH, MIT and Harvard, Cambridge, Massachusetts, USA. Infectious Disease Unit, Massachusetts General Hospital, Boston, Massachusetts, USA.

Address correspondence to: Galit Alter, Ragon Institute of MGH, MIT and Harvard, 400 Technology Square, Cambridge, Massachusetts 02139, USA. Phone: 617.724.0546; Email: galter@mgh.harvard.edu.

Find articles by Alter, G. in: JCI | PubMed | Google Scholar

First published May 21, 2018 - More info

Published in Volume 128, Issue 7 on July 2, 2018
J Clin Invest. 2018;128(7):2743–2745. https://doi.org/10.1172/JCI121053.
Copyright © 2018, American Society for Clinical Investigation
First published May 21, 2018 - Version history

Related article:

Lymphoid tissue fibrosis is associated with impaired vaccine responses
Cissy Kityo, … , Daniel C. Douek, Timothy W. Schacker
Cissy Kityo, … , Daniel C. Douek, Timothy W. Schacker
Research Article Immunology Vaccines

Lymphoid tissue fibrosis is associated with impaired vaccine responses

Abstract

Vaccine responses vary by geographic location. We have previously described how HIV-associated inflammation leads to fibrosis of secondary lymph nodes (LNs) and T cell depletion. We hypothesized that other infections may cause LN inflammation and fibrosis, in a process similar to that seen in HIV infection, which may lead to T cell depletion and affect vaccine responses. We studied LNs of individuals from Kampala, Uganda, before and after yellow fever vaccination (YFV) and found fibrosis in LNs that was similar to that seen in HIV infection. We found blunted antibody responses to YFV that correlated to the amount of LN fibrosis and loss of T cells, including T follicular helper cells. These data suggest that LN fibrosis is not limited to HIV infection and may be associated with impaired immunologic responses to vaccines. This may have an impact on vaccine development, especially for infectious diseases prevalent in the developing world.

Authors

Cissy Kityo, Krystelle Nganou Makamdop, Meghan Rothenberger, Jeffrey G. Chipman, Torfi Hoskuldsson, Gregory J. Beilman, Bartosz Grzywacz, Peter Mugyenyi, Francis Ssali, Rama S. Akondy, Jodi Anderson, Thomas E. Schmidt, Thomas Reimann, Samuel P. Callisto, Jordan Schoephoerster, Jared Schuster, Proscovia Muloma, Patrick Ssengendo, Eirini Moysi, Constantinos Petrovas, Ray Lanciotti, Lin Zhang, Maria T. Arévalo, Benigno Rodriguez, Ted M. Ross, Lydie Trautmann, Rafick-Pierre Sekaly, Michael M. Lederman, Richard A. Koup, Rafi Ahmed, Cavan Reilly, Daniel C. Douek, Timothy W. Schacker

×
Abstract

There is marked variability in vaccine efficacy among global populations. In particular, individuals in low- to middle-income countries have been shown to be less responsive to vaccines than those from developed nations. Several factors, including endemic infections, nutrition, genetics, and gut microbiome composition, have been proposed to underlie discrepancies in vaccine response. In this issue of the JCI, Kityo et al. evaluated response to yellow fever virus vaccine, inflammation, and lymphatic tissue architecture and fibrosis in three cohorts: two from the U.S. and one from Uganda. Compared with the U.S. subjects, the Ugandan cohort exhibited enhanced cytokine responses, increased lymph node fibrosis, reduced CD4+ T cell levels, and reduced vaccine response. Together, these results provide a link among chronic inflammation, damaged lymphoid architecture, and poor vaccine outcome, and set the stage for future studies to identify strategies to overcome these barriers.

A geographic map of vaccine failure

Vaccination against infectious organisms has proven to be one of the most effective public health strategies for controlling and preventing disease. Vaccination strategies have shown specific benefit in low- and middle-income countries, where recent estimates suggest that by 2030 up to 36 million deaths will be averted simply by immunization against 10 common infections (1). Despite the dramatic successes predicted by models, accumulating evidence suggests that vaccine responses vary geographically and that efficacy rates, particularly for oral vaccines against polio, cholera, and rotavirus, are reduced in low- and middle-income countries (2). For example, efficacy rates of Rotarixä, a vaccine against severe rotavirus-induced gastroenteritis, have been reported to be less than 50% for infants vaccinated in Malawi compared with their European counterparts (3). Similarly, deficits in immunogenicity and/or efficacy have been documented for live-attenuated oral cholera vaccines (4) and for the trivalent oral poliovirus vaccine (TOPV), with up to a 30% reduction in seroconversion rates following administration in children in low-income countries compared with children in industrialized countries (5). It has even been suggested that the reduced OPV efficacy in low-income countries has contributed to the delayed global eradication of polio virus.

Interestingly, geographic variation in vaccine responsiveness is not limited to oral vaccines in infants but was described two decades ago for the Bacillus Calmette-Guerin (BCG) vaccine against mycobacterium tuberculosis (TB) (6). BCG immunogenicity has been strongly linked to geographic latitude, with diminished efficacy closer to the equator (7). Similar geographic variation in vaccine immunogenicity was also observed for the yellow fever virus vaccine (YFV), with lower levels of neutralizing antibodies described in an adult Ugandan population compared with a control group in Switzerland (8). Thus, similar geographic disparities have been observed in infants and adults, with both killed and live vaccines, vaccines directed at both bacterial and viral pathogens, and in parenteral and enteric delivery. Together, these discrepancies highlight the potential generalizability of the significant variation in immune responsiveness around the globe. However, while the overall trend of impaired immunogenicity in low-income countries appears to be common to multiple vaccine classes, the mechanism(s) responsible for this phenomenon remain unclear.

Genetics, nutrition, and the gut

A multitude of variables may be involved in shaping the responses to vaccination, with age, sexual phenotype, host genetic factors, environmental influences, nutritional status, and even the microbiome all having been suggested to affect vaccine responsiveness (9–11). Variation in the HLA locus, which encodes the MHC proteins involved in antigen presentation, is among the top host genetic determinants linked to the heterogeneity of vaccine responses. Specific HLA class II alleles have been associated with both increased and reduced or nonresponse to the hepatitis B virus (HBV) vaccine as well as to other vaccines, including those for rubella and measles (12, 13). Additionally, single nucleotide polymorphisms in cytokine and cytokine receptor genes have been shown to affect HBV, tetanus, and pneumococcal vaccine response rates (14). Moreover, twin studies have proposed that genetic variation contributes to as much as 70% of the total observed variability for the HBV (15), measles (16), and oral polio vaccines (17).

In addition to genetic variables, poverty and associated malnourishment have been connected to limited or delayed acquisition of immunity, at least in children, following measles (18), tetanus, and diphtheria vaccination (19). In addition to deficiencies in critical nutrients and the frequent occurrence of enteric dysfunction, the composition of the gut bacterial microbiota itself has been associated with vaccine immunogenicity (11). Significant geographic differences have been linked to microbiome composition (20), and recent data highlight similarities in microbiome composition in rotavirus vaccine responders in Ghana and the Netherlands compared with Ghanaian infant nonresponders (21). Similarly, the specific presence of particular bacterial species in the stool microbiota of Bangladeshi infants, for example, has been associated with enhanced vaccine–specific IgG and T cell proliferative responses to the oral polio, BCG, tetanus toxoid, and HBV vaccines (22). However, results implicating the microbiome in vaccine response variability have thus far been purely associative, and causal links to the microbiota, or information on how it may be exploited to improve immunity, have yet to emerge.

Coinfections: vaccine antagonists

Chronic exposure to and recurrent infections with intestinal helminths, Plasmodium, Mycobacterium, and other endemic pathogens affect more than a third of the global population, with a clear predominance in low-income countries where infection with at least one persistent pathogen is frequently found. Reduced responses to vaccinations for tetanus toxoid (23), cholera (24), and BCG (25) have been associated with helminth coinfection, and children with malaria have been shown to mount weaker antibody responses to tetanus toxoid, Haemophilus influenzae, meningococcal polysaccharide, and Salmonella typhi vaccination (26) than uninfected children. While some parasitic infections do not affect the initial generation or levels of protective antibody, parasitic infections clearly affect the durability of these immune responses, as reported for HBV or tetanus toxoid vaccines in chronic schistosomiasis (27). Several mechanisms have been proposed for the antagonizing effects that coinfection has on vaccine durability. These include skewing of lymphocyte effector functions, induction of antiinflammatory cytokines, altered antigen presenting cell function, and reduced antigen processing and presentation, all of which result in poor or altered priming of adaptive immune responses (reviewed in ref. 28). Moreover, chronic infection and associated inflammation have been associated with altered lymphoid tissue architecture, particularly in HIV infection, and linked to T cell depletion (29). However, the exact consequences of such changes in the context of vaccination have remained poorly understood, until now.

Structural effects on vaccine-induced immunity

In this issue, Kityo et al. (30) specifically explore the role of inflammation, tissue architecture, and fibrosis in modulating vaccine-induced immunity. In a tour de force, the study, which includes lymph node (LN) excisions and immune profiling, specifically aimed to define the linkage among LN inflammation, fibrosis, chronic immune activation, and vaccine-induced immunity. The authors hypothesized that similar to HIV-induced changes in lymphoid architecture (29), recurrent coinfections may contribute to the reduced vaccine responsiveness in low-income countries by altering immune activation–induced lymphoid architecture. Kityo and colleagues (30) recruited three different cohorts to address their hypothesis, specifically 30 HIV– Ugandans, a subset of which was subsequently given YFV; a group of HIV– participants from Atlanta, USA, who received YFV; and a group of 10 HIV– individuals from Minnesota, USA. LNs were collected from the Ugandans and the Minnesotans. Ugandans exhibited enhanced inflammatory cytokine responses, marked by elevated levels of TGF-β, IL-6, IL-4, IL-21, and MIP-1β, compared with subjects from the U.S., suggesting distinct inflammatory profiles across geographic regions of the globe. Additionally, collagen levels were markedly increased in the T cell zone (TZ) in LNs of Ugandans compared with those from U.S. controls, similar to what has been previously reported in HIV+ individuals (29). Furthermore, there was a significant inverse correlation between TZ collagen deposition and TZ CD4+ T cell frequencies, known to be essential drivers of effective vaccine-induced antibody responses, suggesting T cell depletion in the Ugandans, even in the absence of HIV infection. To further determine if the observed differences in lymphatic tissue anatomy were associated with vaccine responsiveness, Kityo and colleagues (30) examined draining LNs 10–14 days after YFV and measured plasma YFV antibody titers by plaque reduction assay. Interestingly, not only was there a relative lack of primary follicles with fewer secondary follicles (germinal centers [GCs]) prior to vaccination in the Ugandan cohort, but also the majority of these individuals did not have a detectable increase in primary or secondary follicle formation following vaccination. Moreover, these immunological defects were consistent with a poor antibody response in the Ugandan cohort and correlated with increased damage in the fibroblastic reticular cell network (FRCn), an anatomic correlate of poor vaccine response. Thus, these data provide a definitive link among chronic inflammation, damaged lymphoid architecture, and poor vaccine outcome (Figure 1).

Factors driving lower vaccine responsiveness around the globe.Figure 1

Factors driving lower vaccine responsiveness around the globe. The cartoon bar graph depicts the observed differences in vaccine responsiveness around the globe, and highlights factors that have been associated with vaccine immunogenicity. Factors listed in black have been previously published. Factors listed in red represent the findings in the manuscript by Kityo et al., which evaluated differences in vaccine response in subjects from the U.S. (Minnesota and Georgia, blue) and subjects from Uganda (red).

Summary and future directions

Vaccine immunogenicity is frequently reduced in vulnerable populations, including those living in low-income countries, the elderly, and other marginalized populations (e.g., drug users) in the developed world. A common thread among many of these populations is the marked elevation and/or alteration of inflammation. The results presented by Kityo et al. (30) now propose a mechanism that may transcend across global populations and may provide novel insights into dampened vaccine immunity, with particular focus on the role of inflammation in shaping LN anatomy. Specifically, increased inflammation is associated with fibrotic damage to the TZ, resulting in dampened ability for GC generation and priming of vaccine-specific antibodies. The insights of Kityo et al. provide critical clues to the vaccine field and identify a target obstacle that must be overcome to attain comparable immunity at a global level. While attempts to reduce or reverse LN fibrosis in HIV+ individuals with antifibrotic agents like the angiotensin receptor blocker and PPAR-γ agonist telmisartan have been unsuccessful (31), modified vaccine regimens, novel adjuvants, as well as next-generation vaccine delivery strategies may counter the limitations of fibrotic lymph tissues. Thus, the study by Kityo et al. represents a significant step in defining the immune system’s Achilles’ heel and is a critical first step in achieving global immunity to prevent the devastation of infectious diseases.

Acknowledgments

This work was supported by NIH K08 AI 106408 (BJ).

Footnotes

Conflict of interest: The authors have declared that no conflict of interest exists.

Reference information: J Clin Invest. 2018;128(7):2743–2745. https://doi.org/10.1172/JCI121053.

See the related article at Lymphoid tissue fibrosis is associated with impaired vaccine responses.

References
  1. Chang AY, et al. The equity impact vaccines may have on averting deaths and medical impoverishment in developing countries. Health Aff (Millwood). 2018;37(2):316–324.
    View this article via: PubMed CrossRef Google Scholar
  2. Cherian T, Wang S, Mantel C. Rotavirus vaccines in developing countries: the potential impact, implementation challenges, and remaining questions. Vaccine. 2012;30 Suppl 1:A3–A6.
    View this article via: PubMed Google Scholar
  3. Madhi SA, et al. Effect of human rotavirus vaccine on severe diarrhea in African infants. N Engl J Med. 2010;362(4):289–298.
    View this article via: PubMed CrossRef Google Scholar
  4. Suharyono , et al. Safety and immunogenicity of single-dose live oral cholera vaccine CVD 103-HgR in 5-9-year-old Indonesian children. Lancet. 1992;340(8821):689–694.
    View this article via: PubMed CrossRef Google Scholar
  5. Patriarca PA, Wright PF, John TJ. Factors affecting the immunogenicity of oral poliovirus vaccine in developing countries: review. Rev Infect Dis. 1991;13(5):926–939.
    View this article via: PubMed CrossRef Google Scholar
  6. Fine PE. Variation in protection by BCG: implications of and for heterologous immunity. Lancet. 1995;346(8986):1339–1345.
    View this article via: PubMed CrossRef Google Scholar
  7. Black GF, et al. BCG-induced increase in interferon-gamma response to mycobacterial antigens and efficacy of BCG vaccination in Malawi and the UK: two randomised controlled studies. Lancet. 2002;359(9315):1393–1401.
    View this article via: PubMed CrossRef Google Scholar
  8. Muyanja E, et al. Immune activation alters cellular and humoral responses to yellow fever 17D vaccine. J Clin Invest. 2014;124(7):3147–3158.
    View this article via: JCI PubMed CrossRef Google Scholar
  9. Mentzer AJ, O’Connor D, Pollard AJ, Hill AV. Searching for the human genetic factors standing in the way of universally effective vaccines. Philos Trans R Soc Lond, B, Biol Sci. 2015;370(1671):20140341.
    View this article via: PubMed Google Scholar
  10. Flanagan KL, Fink AL, Plebanski M, Klein SL. Sex and gender differences in the outcomes of vaccination over the life course. Annu Rev Cell Dev Biol. 2017;33:577–599.
    View this article via: PubMed CrossRef Google Scholar
  11. Lynn DJ, Pulendran B. The potential of the microbiota to influence vaccine responses. J Leukoc Biol. 2018;103(2):225–231.
    View this article via: PubMed Google Scholar
  12. Yucesoy B, et al. Genetic variants within the MHC region are associated with immune responsiveness to childhood vaccinations. Vaccine. 2013;31(46):5381–5391.
    View this article via: PubMed CrossRef Google Scholar
  13. Ovsyannikova IG, Dhiman N, Jacobson RM, Poland GA. Human leukocyte antigen polymorphisms: variable humoral immune responses to viral vaccines. Expert Rev Vaccines. 2006;5(1):33–43.
    View this article via: PubMed CrossRef Google Scholar
  14. Yucesoy B, et al. Influence of cytokine gene variations on immunization to childhood vaccines. Vaccine. 2009;27(50):6991–6997.
    View this article via: PubMed CrossRef Google Scholar
  15. Höhler T, et al. Differential genetic determination of immune responsiveness to hepatitis B surface antigen and to hepatitis A virus: a vaccination study in twins. Lancet. 2002;360(9338):991–995.
    View this article via: PubMed CrossRef Google Scholar
  16. Tan PL, Jacobson RM, Poland GA, Jacobsen SJ, Pankratz VS. Twin studies of immunogenicity — determining the genetic contribution to vaccine failure. Vaccine. 2001;19(17-19):2434–2439.
    View this article via: PubMed CrossRef Google Scholar
  17. Newport MJ, et al. Genetic regulation of immune responses to vaccines in early life. Genes Immun. 2004;5(2):122–129.
    View this article via: PubMed CrossRef Google Scholar
  18. Powell GM. Response to live attenuated measles vaccine in children with severe kwashiorkor. Ann Trop Paediatr. 1982;2(3):143–145.
    View this article via: PubMed CrossRef Google Scholar
  19. Brüssow H, Sidoti J, Dirren H, Freire WB. Effect of malnutrition in Ecuadorian children on titers of serum antibodies to various microbial antigens. Clin Diagn Lab Immunol. 1995;2(1):62–68.
    View this article via: PubMed Google Scholar
  20. Yatsunenko T, et al. Human gut microbiome viewed across age and geography. Nature. 2012;486(7402):222–227.
    View this article via: PubMed CrossRef Google Scholar
  21. Harris VC, et al. Significant correlation between the infant gut microbiome and rotavirus vaccine response in rural Ghana. J Infect Dis. 2017;215(1):34–41.
    View this article via: PubMed CrossRef Google Scholar
  22. Huda MN, et al. Stool microbiota and vaccine responses of infants. Pediatrics. 2014;134(2):e362–e372.
    View this article via: PubMed CrossRef Google Scholar
  23. Nookala S, Srinivasan S, Kaliraj P, Narayanan RB, Nutman TB. Impairment of tetanus-specific cellular and humoral responses following tetanus vaccination in human lymphatic filariasis. Infect Immun. 2004;72(5):2598–2604.
    View this article via: PubMed CrossRef Google Scholar
  24. Cooper PJ, et al. Human infection with Ascaris lumbricoides is associated with suppression of the interleukin-2 response to recombinant cholera toxin B subunit following vaccination with the live oral cholera vaccine CVD 103-HgR. Infect Immun. 2001;69(3):1574–1580.
    View this article via: PubMed CrossRef Google Scholar
  25. Elias D, Wolday D, Akuffo H, Petros B, Bronner U, Britton S. Effect of deworming on human T cell responses to mycobacterial antigens in helminth-exposed individuals before and after bacille Calmette-Guérin (BCG) vaccination. Clin Exp Immunol. 2001;123(2):219–225.
    View this article via: PubMed CrossRef Google Scholar
  26. Cunnington AJ, Riley EM. Suppression of vaccine responses by malaria: insignificant or overlooked? Expert Rev Vaccines. 2010;9(4):409–429.
    View this article via: PubMed CrossRef Google Scholar
  27. Riner DK, et al. Schistosoma mansoni infection can jeopardize the duration of protective levels of antibody responses to immunizations against hepatitis B and tetanus toxoid. PLoS Negl Trop Dis. 2016;10(12):e0005180.
    View this article via: PubMed CrossRef Google Scholar
  28. Stelekati E, Wherry EJ. Chronic bystander infections and immunity to unrelated antigens. Cell Host Microbe. 2012;12(4):458–469.
    View this article via: PubMed CrossRef Google Scholar
  29. Schacker TW, et al. Collagen deposition in HIV-1 infected lymphatic tissues and T cell homeostasis. J Clin Invest. 2002;110(8):1133–1139.
    View this article via: JCI PubMed CrossRef Google Scholar
  30. Kityo C, et al. Lymphoid tissue fibrosis is associated with impaired vaccine responses. J Clin Invest. 2018;128(7):2763–2773.
    View this article via: JCI PubMed CrossRef Google Scholar
  31. Utay NS, et al. Telmisartan does not improve lymph node or adipose tissue fibrosis more than continued ART alone. J Infect Dis. 2018;217(11):1770–1781.
    View this article via: PubMed CrossRef Google Scholar
Version history
  • Version 1 (May 21, 2018): Electronic publication
  • Version 2 (July 2, 2018): Print issue publication
Advertisement
Advertisement