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Research Article Free access | 10.1172/JCI119302

Correcting temperature-sensitive protein folding defects.

C R Brown, L Q Hong-Brown, and W J Welch

Department of Medicine, The University of California, San Francisco 94143, USA. crb@itsa.ucsf.edu

Find articles by Brown, C. in: JCI | PubMed | Google Scholar

Department of Medicine, The University of California, San Francisco 94143, USA. crb@itsa.ucsf.edu

Find articles by Hong-Brown, L. in: JCI | PubMed | Google Scholar

Department of Medicine, The University of California, San Francisco 94143, USA. crb@itsa.ucsf.edu

Find articles by Welch, W. in: JCI | PubMed | Google Scholar

Published March 15, 1997 - More info

Published in Volume 99, Issue 6 on March 15, 1997
J Clin Invest. 1997;99(6):1432–1444. https://doi.org/10.1172/JCI119302.
© 1997 The American Society for Clinical Investigation
Published March 15, 1997 - Version history
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Abstract

Recently, we found that different low molecular weight compounds, all known to stabilize proteins in their native conformation, are effective in correcting the temperature-sensitive protein folding defect associated with the deltaF508 cystic fibrosis transmembrane regulator (CFTR) protein. Here we examined whether the folding of other proteins which exhibit temperature-sensitive folding defects also could be corrected via a similar strategy. Cell lines expressing temperature-sensitive mutants of the tumor suppressor protein p53, the viral oncogene protein pp60src, or a ubiquitin activating enzyme E1, were incubated at the nonpermissive temperature (39.5 degrees C) in the presence of glycerol, trimethylamine N-oxide or deuterated water. In each case, the cells exhibited phenotypes similar to those observed when the cells were incubated at the permissive temperature (32.5 degrees C), indicative that the particular protein folding defect had been corrected. These observations, coupled with our earlier work and much older studies in yeast and bacteria, indicate that protein stabilizing agents are effective in vivo for correcting protein folding abnormalities. We suggest that this type of approach may prove to be useful for correcting certain protein folding abnormalities associated with human diseases.

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