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Research Article Free access | 10.1172/JCI115251

Repeated glucose stimulation reveals distinct and lasting secretion patterns of individual rat pancreatic B cells.

E Giordano, D Bosco, V Cirulli, and P Meda

Department of Morphology, University of Geneva Medical School, Switzerland.

Find articles by Giordano, E. in: JCI | PubMed | Google Scholar

Department of Morphology, University of Geneva Medical School, Switzerland.

Find articles by Bosco, D. in: JCI | PubMed | Google Scholar

Department of Morphology, University of Geneva Medical School, Switzerland.

Find articles by Cirulli, V. in: JCI | PubMed | Google Scholar

Department of Morphology, University of Geneva Medical School, Switzerland.

Find articles by Meda, P. in: JCI | PubMed | Google Scholar

Published June 1, 1991 - More info

Published in Volume 87, Issue 6 on June 1, 1991
J Clin Invest. 1991;87(6):2178–2185. https://doi.org/10.1172/JCI115251.
© 1991 The American Society for Clinical Investigation
Published June 1, 1991 - Version history
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Abstract

To determine whether pancreatic B cells show a constant secretion pattern during repeated stimulations, we have used a sequential hemolytic plaque assay to monitor their individual insulin release during several successive 30-min incubations in the presence of 16.7 mM glucose. We have found that the total B cell secretion did not vary significantly in these successive glucose stimulations and that, under these conditions, the majority of B cells that were stimulated to release insulin during the first incubation also secreted during the second, third, and, when this was tested, during the fourth incubation. Similarly, most of the B cells that did not release detectable amounts of insulin during the first incubation did not secrete also during the two (or three) subsequent secretion tests. Together, the two groups of B cells that showed a constant secretory pattern, represented approximately 75% of the entire B cell population. The remaining 25% of B cells shifted from a secreting to a non-secreting state, or vice versa, from one incubation to another. These observations were made under three different time frames in which we tested single B cells as well as B cell clusters at rather different intervals. These findings support the existence of distinct B cell subpopulations differing lastingly in their ability to secrete insulin in response to glucose.

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