Go to JCI Insight
  • About
  • Editors
  • Consulting Editors
  • For authors
  • Publication ethics
  • Alerts
  • Advertising
  • Job board
  • Subscribe
  • Contact
  • Current issue
  • Past issues
  • By specialty
    • COVID-19
    • Cardiology
    • Gastroenterology
    • Immunology
    • Metabolism
    • Nephrology
    • Neuroscience
    • Oncology
    • Pulmonology
    • Vascular biology
    • All ...
  • Videos
    • Conversations with Giants in Medicine
    • Author's Takes
  • Reviews
    • View all reviews ...
    • Immune Environment in Glioblastoma (Feb 2023)
    • Korsmeyer Award 25th Anniversary Collection (Jan 2023)
    • Aging (Jul 2022)
    • Next-Generation Sequencing in Medicine (Jun 2022)
    • New Therapeutic Targets in Cardiovascular Diseases (Mar 2022)
    • Immunometabolism (Jan 2022)
    • Circadian Rhythm (Oct 2021)
    • View all review series ...
  • Viewpoint
  • Collections
    • In-Press Preview
    • Commentaries
    • Research letters
    • Letters to the editor
    • Editorials
    • Viewpoint
    • Top read articles
  • Clinical Medicine
  • JCI This Month
    • Current issue
    • Past issues

  • Current issue
  • Past issues
  • Specialties
  • Reviews
  • Review series
  • Conversations with Giants in Medicine
  • Author's Takes
  • In-Press Preview
  • Commentaries
  • Research letters
  • Letters to the editor
  • Editorials
  • Viewpoint
  • Top read articles
  • About
  • Editors
  • Consulting Editors
  • For authors
  • Publication ethics
  • Alerts
  • Advertising
  • Job board
  • Subscribe
  • Contact
Top
  • View PDF
  • Download citation information
  • Send a comment
  • Share this article
  • Terms of use
  • Standard abbreviations
  • Need help? Email the journal
  • Top
  • Abstract
  • Version history
  • Article usage
  • Citations to this article

Advertisement

Research Article Free access | 10.1172/JCI115166

Expression of mRNA for interleukin-5 in mucosal bronchial biopsies from asthma.

Q Hamid, M Azzawi, S Ying, R Moqbel, A J Wardlaw, C J Corrigan, B Bradley, S R Durham, J V Collins, and P K Jeffery

Department of Allergy and Clinical Immunology, National Heart and Lung Institute, London, United Kingdom.

Find articles by Hamid, Q. in: JCI | PubMed | Google Scholar

Department of Allergy and Clinical Immunology, National Heart and Lung Institute, London, United Kingdom.

Find articles by Azzawi, M. in: JCI | PubMed | Google Scholar

Department of Allergy and Clinical Immunology, National Heart and Lung Institute, London, United Kingdom.

Find articles by Ying, S. in: JCI | PubMed | Google Scholar

Department of Allergy and Clinical Immunology, National Heart and Lung Institute, London, United Kingdom.

Find articles by Moqbel, R. in: JCI | PubMed | Google Scholar

Department of Allergy and Clinical Immunology, National Heart and Lung Institute, London, United Kingdom.

Find articles by Wardlaw, A. in: JCI | PubMed | Google Scholar

Department of Allergy and Clinical Immunology, National Heart and Lung Institute, London, United Kingdom.

Find articles by Corrigan, C. in: JCI | PubMed | Google Scholar

Department of Allergy and Clinical Immunology, National Heart and Lung Institute, London, United Kingdom.

Find articles by Bradley, B. in: JCI | PubMed | Google Scholar

Department of Allergy and Clinical Immunology, National Heart and Lung Institute, London, United Kingdom.

Find articles by Durham, S. in: JCI | PubMed | Google Scholar

Department of Allergy and Clinical Immunology, National Heart and Lung Institute, London, United Kingdom.

Find articles by Collins, J. in: JCI | PubMed | Google Scholar

Department of Allergy and Clinical Immunology, National Heart and Lung Institute, London, United Kingdom.

Find articles by Jeffery, P. in: JCI | PubMed | Google Scholar

Published May 1, 1991 - More info

Published in Volume 87, Issue 5 on May 1, 1991
J Clin Invest. 1991;87(5):1541–1546. https://doi.org/10.1172/JCI115166.
© 1991 The American Society for Clinical Investigation
Published May 1, 1991 - Version history
View PDF
Abstract

We have attempted to identify mRNA for IL-5 in endobronchial mucosal biopsies from asthmatics and controls, using the technique of in situ hybridization. Bronchial biopsies were obtained from 10 asthmatics and 9 nonatopic normal controls. A radio-labeled cRNA probe was prepared from an IL-5 cDNA and hybridized to permeabilized sections. These were washed extensively before processing for autoradiography. An IL-5-producing T cell clone derived from a patient with the hyper-IgE syndrome was used as a positive control. As a negative control, sections were also treated with a "sense" IL-5 probe. Specific hybridization signals for IL-5 mRNA were demonstrated within the bronchial mucosa in 6 out of the 10 asthmatic subjects. Cells exhibiting hybridization signals were located beneath the epithelial basement membrane. In contrast, there was no hybridization in the control group. No hybridization was observed with the sense probe. The six IL-5 mRNA-positive asthmatics tended to have more severe disease than the negative asthmatics, as assessed by symptoms and lung function, and showed a significant increase in the degree of infiltration of the bronchial mucosa by secreting (EG2+) eosinophils and activated (CD25+) T lymphocytes. Within the subjects who showed positive IL-5 mRNA, there was a correlation between IL-5 mRNA expression and the number of CD25+ and EG2+ cells and total eosinophil count. This study provides evidence for the cellular localization of IL-5 mRNA in the bronchial mucosa of asthmatics and supports the concept that this cytokine regulates eosinophil function in bronchial asthma.

Images.

Browse pages

Click on an image below to see the page. View PDF of the complete article

icon of scanned page 1541
page 1541
icon of scanned page 1542
page 1542
icon of scanned page 1543
page 1543
icon of scanned page 1544
page 1544
icon of scanned page 1545
page 1545
icon of scanned page 1546
page 1546
Version history
  • Version 1 (May 1, 1991): No description

Article tools

  • View PDF
  • Download citation information
  • Send a comment
  • Share this article
  • Terms of use
  • Standard abbreviations
  • Need help? Email the journal

Metrics

  • Article usage
  • Citations to this article

Go to

  • Top
  • Abstract
  • Version history
Advertisement
Advertisement

Copyright © 2023 American Society for Clinical Investigation
ISSN: 0021-9738 (print), 1558-8238 (online)

Sign up for email alerts