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Research Article Free access | 10.1172/JCI112809

Autoantibody to RNA polymerase I in scleroderma sera.

G Reimer, K M Rose, U Scheer, and E M Tan

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Published January 1, 1987 - More info

Published in Volume 79, Issue 1 on January 1, 1987
J Clin Invest. 1987;79(1):65–72. https://doi.org/10.1172/JCI112809.
© 1987 The American Society for Clinical Investigation
Published January 1, 1987 - Version history
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Abstract

Autoantibodies to components of the nucleolus are a unique serological feature of patients with scleroderma. There are autoantibodies of several specificities; one type produces a speckled pattern of nucleolar staining in immunofluorescence. In actinomycin D and 5,6-dichloro-beta-D-ribofuranosylbenzimidazole-treated Vero cells, staining was restricted to the fibrillar and not the granular regions. By double immunofluorescence, specific rabbit anti-RNA polymerase I antibodies stained the same fibrillar structures in drug-segregated nucleoli as scleroderma sera. Scleroderma sera immunoprecipitated 13 polypeptides from [35S]methionine-labeled HeLa cell extract with molecular weights ranging from 210,000 to 14,000. Similar polypeptides were precipitated by rabbit anti-RNA polymerase I antibodies, and their common identities were confirmed in immunoabsorption experiments. Microinjection of purified IgG from a patient with speckled nucleolar staining effectively inhibited ribosomal RNA transcription. Autoantibodies to RNA polymerase I were restricted to certain patients with scleroderma and were not found in other autoimmune diseases.

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