Go to JCI Insight
  • About
  • Editors
  • Consulting Editors
  • For authors
  • Publication ethics
  • Publication alerts by email
  • Advertising
  • Job board
  • Contact
  • Clinical Research and Public Health
  • Current issue
  • Past issues
  • By specialty
    • COVID-19
    • Cardiology
    • Gastroenterology
    • Immunology
    • Metabolism
    • Nephrology
    • Neuroscience
    • Oncology
    • Pulmonology
    • Vascular biology
    • All ...
  • Videos
    • Conversations with Giants in Medicine
    • Video Abstracts
  • Reviews
    • View all reviews ...
    • Complement Biology and Therapeutics (May 2025)
    • Evolving insights into MASLD and MASH pathogenesis and treatment (Apr 2025)
    • Microbiome in Health and Disease (Feb 2025)
    • Substance Use Disorders (Oct 2024)
    • Clonal Hematopoiesis (Oct 2024)
    • Sex Differences in Medicine (Sep 2024)
    • Vascular Malformations (Apr 2024)
    • View all review series ...
  • Viewpoint
  • Collections
    • In-Press Preview
    • Clinical Research and Public Health
    • Research Letters
    • Letters to the Editor
    • Editorials
    • Commentaries
    • Editor's notes
    • Reviews
    • Viewpoints
    • 100th anniversary
    • Top read articles

  • Current issue
  • Past issues
  • Specialties
  • Reviews
  • Review series
  • Conversations with Giants in Medicine
  • Video Abstracts
  • In-Press Preview
  • Clinical Research and Public Health
  • Research Letters
  • Letters to the Editor
  • Editorials
  • Commentaries
  • Editor's notes
  • Reviews
  • Viewpoints
  • 100th anniversary
  • Top read articles
  • About
  • Editors
  • Consulting Editors
  • For authors
  • Publication ethics
  • Publication alerts by email
  • Advertising
  • Job board
  • Contact
Top
  • View PDF
  • Download citation information
  • Send a comment
  • Terms of use
  • Standard abbreviations
  • Need help? Email the journal
  • Top
  • Abstract
  • Version history
  • Article usage
  • Citations to this article

Advertisement

Research Article Free access | 10.1172/JCI107283

Specific Biologic Effects of Intestinal Glucagon-Like Materials

Raul A. Gutman, Gloria Fink, Nancy Voyles, Helena Selawry, Juan C. Penhos, Albert Lepp, and Lillian Recant

Veterans Administration Hospital, Washington, D. C. 20422

Department of Medicine, Georgetown University, Washington, D. C. 20007

Department of Physiology, Georgetown University, Washington, D. C. 20007

Find articles by Gutman, R. in: PubMed | Google Scholar

Veterans Administration Hospital, Washington, D. C. 20422

Department of Medicine, Georgetown University, Washington, D. C. 20007

Department of Physiology, Georgetown University, Washington, D. C. 20007

Find articles by Fink, G. in: PubMed | Google Scholar

Veterans Administration Hospital, Washington, D. C. 20422

Department of Medicine, Georgetown University, Washington, D. C. 20007

Department of Physiology, Georgetown University, Washington, D. C. 20007

Find articles by Voyles, N. in: PubMed | Google Scholar

Veterans Administration Hospital, Washington, D. C. 20422

Department of Medicine, Georgetown University, Washington, D. C. 20007

Department of Physiology, Georgetown University, Washington, D. C. 20007

Find articles by Selawry, H. in: PubMed | Google Scholar

Veterans Administration Hospital, Washington, D. C. 20422

Department of Medicine, Georgetown University, Washington, D. C. 20007

Department of Physiology, Georgetown University, Washington, D. C. 20007

Find articles by Penhos, J. in: PubMed | Google Scholar

Veterans Administration Hospital, Washington, D. C. 20422

Department of Medicine, Georgetown University, Washington, D. C. 20007

Department of Physiology, Georgetown University, Washington, D. C. 20007

Find articles by Lepp, A. in: PubMed | Google Scholar

Veterans Administration Hospital, Washington, D. C. 20422

Department of Medicine, Georgetown University, Washington, D. C. 20007

Department of Physiology, Georgetown University, Washington, D. C. 20007

Find articles by Recant, L. in: PubMed | Google Scholar

Published May 1, 1973 - More info

Published in Volume 52, Issue 5 on May 1, 1973
J Clin Invest. 1973;52(5):1165–1175. https://doi.org/10.1172/JCI107283.
© 1973 The American Society for Clinical Investigation
Published May 1, 1973 - Version history
View PDF
Abstract

It has been demonstrated that gastrointestinal extracts contain substances which react immunologically with antibodies prepared to pancreatic glucagon. These extracts have been termed intestinal GLI for glucagon-like immunoreactivity, or enteroglucagon. To determine whether GLI has specific biological effects, studies were designed using the criterion of effect with antiglucagon antibodies. These antibodies did not cross-react with either secretin or pancreozymin.

Rat intestinal extracts were prepared and filtered on Sephadex G-50 columns eluted in 0.02 M ammonium carbonate buffer pH 8.8. Two peaks of GLI (I, II) were consistently found, and the in vitro effects of these peaks on two biological systems were tested: (a) immunoreactive insulin (IRI) release by rat pancreas pieces, and (b) free fatty acid (FFA) release and 3′,5′-cyclic adenosine monophosphate (cAMP) levels in adipose tissue. Both GLI peaks increased IRI release in the absence of glucose and also enhanced the glucose effects. Antiglucagon antibody suppressed only peak II GLI activity. Both peaks increased FFA release and cAMP levels in adipose tissue. Only peak II GLI activity was suppressed by antibody.

These findings support a specific IRI-releasing and lipolytic action for Peak II GLI. Hypotheses are presented concerning the structure and possible physiologic role of peak II GLI.

Browse pages

Click on an image below to see the page. View PDF of the complete article

icon of scanned page 1165
page 1165
icon of scanned page 1166
page 1166
icon of scanned page 1167
page 1167
icon of scanned page 1168
page 1168
icon of scanned page 1169
page 1169
icon of scanned page 1170
page 1170
icon of scanned page 1171
page 1171
icon of scanned page 1172
page 1172
icon of scanned page 1173
page 1173
icon of scanned page 1174
page 1174
icon of scanned page 1175
page 1175
Version history
  • Version 1 (May 1, 1973): No description

Article tools

  • View PDF
  • Download citation information
  • Send a comment
  • Terms of use
  • Standard abbreviations
  • Need help? Email the journal

Metrics

  • Article usage
  • Citations to this article

Go to

  • Top
  • Abstract
  • Version history
Advertisement
Advertisement

Copyright © 2025 American Society for Clinical Investigation
ISSN: 0021-9738 (print), 1558-8238 (online)

Sign up for email alerts