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Infusion of long-chain fatty acid anions by continuous-flow centrifugation
William B. Greenough III, Stephen R. Crespin, Daniel Steinberg
William B. Greenough III, Stephen R. Crespin, Daniel Steinberg
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Research Article

Infusion of long-chain fatty acid anions by continuous-flow centrifugation

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Abstract

We have developed a method for the rapid infusion into plasma of large amounts of long-chain free fatty acids (FFA). Unanesthetized dogs were connected by a peripheral artery to a closed, continuousflow centrifuge from which cells and plasma emerged in separate lines. Sodium oleate was infused directly into the plasma line before cells and plasma were recombined and returned to the animal through a peripheral vein.

Authors

William B. Greenough III, Stephen R. Crespin, Daniel Steinberg

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