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Drug-induced erythrocyte membrane internalization
Isaac Ben-Bassat, Klaus G. Bensch, Stanley L. Schrier
Isaac Ben-Bassat, Klaus G. Bensch, Stanley L. Schrier
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Research Article

Drug-induced erythrocyte membrane internalization

Abstract

In vitro erythrocyte membrane internalization, resulting in the formation of membrane-lined vacuoles, can be quantified by a radioisotopic method. A complex of 37Co-labeled vitamin B12 and its plasma protein binders is first adsorbed to the cell surface, and after vacuoles are formed, the noninternalized label is removed by washing and trypsin treatment. The residual radioactivity represents trapped label and can be used to measure the extent of membrane internalization.

Authors

Isaac Ben-Bassat, Klaus G. Bensch, Stanley L. Schrier

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