Advertisement

Research Article Free access | 10.1172/JCI824

A multidrug-resistance protein (MRP)-like transmembrane pump is highly expressed by resting murine T helper (Th) 2, but not Th1 cells, and is induced to equal expression levels in Th1 and Th2 cells after antigenic stimulation in vivo.

M Lohoff, S Prechtl, F Sommer, M Roellinghoff, E Schmitt, G Gradehandt, P Rohwer, B D Stride, S P Cole, and R G Deeley

Institut für Klinische Mikrobiologie und Immunologie, University of Erlangen, 91054 Erlangen, Germany.

Find articles by Lohoff, M. in: PubMed | Google Scholar

Institut für Klinische Mikrobiologie und Immunologie, University of Erlangen, 91054 Erlangen, Germany.

Find articles by Prechtl, S. in: PubMed | Google Scholar

Institut für Klinische Mikrobiologie und Immunologie, University of Erlangen, 91054 Erlangen, Germany.

Find articles by Sommer, F. in: PubMed | Google Scholar

Institut für Klinische Mikrobiologie und Immunologie, University of Erlangen, 91054 Erlangen, Germany.

Find articles by Roellinghoff, M. in: PubMed | Google Scholar

Institut für Klinische Mikrobiologie und Immunologie, University of Erlangen, 91054 Erlangen, Germany.

Find articles by Schmitt, E. in: PubMed | Google Scholar

Institut für Klinische Mikrobiologie und Immunologie, University of Erlangen, 91054 Erlangen, Germany.

Find articles by Gradehandt, G. in: PubMed | Google Scholar

Institut für Klinische Mikrobiologie und Immunologie, University of Erlangen, 91054 Erlangen, Germany.

Find articles by Rohwer, P. in: PubMed | Google Scholar

Institut für Klinische Mikrobiologie und Immunologie, University of Erlangen, 91054 Erlangen, Germany.

Find articles by Stride, B. in: PubMed | Google Scholar

Institut für Klinische Mikrobiologie und Immunologie, University of Erlangen, 91054 Erlangen, Germany.

Find articles by Cole, S. in: PubMed | Google Scholar

Institut für Klinische Mikrobiologie und Immunologie, University of Erlangen, 91054 Erlangen, Germany.

Find articles by Deeley, R. in: PubMed | Google Scholar

Published February 1, 1998 - More info

Published in Volume 101, Issue 3 on February 1, 1998
J Clin Invest. 1998;101(3):703–710. https://doi.org/10.1172/JCI824.
© 1998 The American Society for Clinical Investigation
Published February 1, 1998 - Version history
View PDF
Abstract

A transmembrane pump for organic anions was identified in resting murine T helper (Th) 2, but not Th1 lymphocyte cell clones, as revealed by extrusion of a fluorescent dye. Dye extrusion inhibition studies suggested that the pump may be the multidrug-resistance protein (MRP). The different expression of the pump in resting Th1 and Th2 cell clones correlated with their respective levels of MRP mRNA. The pump was inducible in Th1 cells by antigenic stimulation in vitro leading to equal expression in activated Th1 and Th2 cell clones. This suggested that dye extrusion might allow the detection of Th2 (resting or activated) or of activated Th1 cells ex vivo based on a functional parameter. To test this, mice were infected with Leishmania major parasites to activate L. major-specific T cells of either Th1 (C57BL/6 mice) or Th2 (BALB/c mice) phenotype: 2-3% of CD4+ lymph node T cells of both strains of mice extruded the dye, defining a cell subset that did not coincide with subsets defined by other activation markers. Fluorescence-activated cell-sorting revealed that the lymphokine response (Th1 or Th2, respectively) to L. major antigens was restricted to this dye-extruding subset.

Version history
  • Version 1 (February 1, 1998): No description
Advertisement
Advertisement