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SDF1 induction by acidosis from principal cells regulates intercalated cell subtype distribution
George J. Schwartz, … , Dominique Eladari, Qais Al-Awqati
George J. Schwartz, … , Dominique Eladari, Qais Al-Awqati
Published October 26, 2015
Citation Information: J Clin Invest. 2015;125(12):4365-4374. https://doi.org/10.1172/JCI80225.
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Research Article Nephrology

SDF1 induction by acidosis from principal cells regulates intercalated cell subtype distribution

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Abstract

The nephron cortical collecting duct (CCD) is composed of principal cells, which mediate Na, K, and water transport, and intercalated cells (ICs), which are specialized for acid-base transport. There are two canonical IC forms: acid-secreting α-ICs and HCO3-secreting β-ICs. Chronic acidosis increases α-ICs at the expense of β-ICs, thereby increasing net acid secretion by the CCD. We found by growth factor quantitative PCR array that acidosis increases expression of mRNA encoding SDF1 (or CXCL12) in kidney cortex and isolated CCDs from mouse and rabbit kidney cortex. Exogenous SDF1 or pH 6.8 media increased H+ secretion and decreased HCO3 secretion in isolated perfused rabbit CCDs. Acid-dependent changes in H+ and HCO3 secretion were largely blunted by AMD3100, which selectively blocks the SDF1 receptor CXCR4. In mice, diet-induced chronic acidosis increased α-ICs and decreased β-ICs. Additionally, IC-specific Cxcr4 deletion prevented IC subtype alterations and magnified metabolic acidosis. SDF1 was transcriptionally regulated and a target of the hypoxia-sensing transcription factor HIF1α. IC-specific deletion of Hif1a produced no effect on mice fed an acid diet, as α-ICs increased and β-ICs decreased similarly to that observed in WT littermates. However, Hif1a deletion in all CCD cells prevented acidosis-induced IC subtype distribution, resulting in more severe acidosis. Cultured principal cells exhibited an HIF1α-dependent increase of Sdf1 transcription in response to media acidification. Thus, our results indicate that principal cells respond to acid by producing SDF1, which then acts on adjacent ICs.

Authors

George J. Schwartz, XiaoBo Gao, Shuichi Tsuruoka, Jeffrey M. Purkerson, Hu Peng, Vivette D’Agati, Nicolas Picard, Dominique Eladari, Qais Al-Awqati

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Figure 4

Acid-base transport in isolated perfused rabbit CCDs: effect of the CXCR4 antagonist AMD3100.

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Acid-base transport in isolated perfused rabbit CCDs: effect of the CXCR...
Tubules were perfused and fluxes were measured at pH 7.4, following which the bathing medium was replaced with the same solution, except at pH 6.8. Three hours later, fluxes were measured again. (A) Initial fluxes at pH 7.4 (blue bars) and effect of a 3-hour incubation at pH 6.8 (red bars); n = 6. The difference between these 2 fluxes is indicated in the green bars. (B) Same as in A, except the medium contained 1 μM AMD3100; n = 6. (C) Initial fluxes at pH 7.4 (blue bars) and the effect of a 3-hour incubation at pH 7.4 (aqua bars); n = 3. Data represent the average ± SEM. Numerical values of the mean ± SEM are provided in Table 2.

Copyright © 2023 American Society for Clinical Investigation
ISSN: 0021-9738 (print), 1558-8238 (online)

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