NOTCH pathway inactivation promotes bladder cancer progression
Antonio Maraver, … , Francisco X. Real, Manuel Serrano
Antonio Maraver, … , Francisco X. Real, Manuel Serrano
Published February 2, 2015; First published January 9, 2015
Citation Information: J Clin Invest. 2015;125(2):824-830. https://doi.org/10.1172/JCI78185.
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Research Article

NOTCH pathway inactivation promotes bladder cancer progression

Abstract

NOTCH signaling suppresses tumor growth and proliferation in several types of stratified epithelia. Here, we show that missense mutations in NOTCH1 and NOTCH2 found in human bladder cancers result in loss of function. In murine models, genetic ablation of the NOTCH pathway accelerated bladder tumorigenesis and promoted the formation of squamous cell carcinomas, with areas of mesenchymal features. Using bladder cancer cells, we determined that the NOTCH pathway stabilizes the epithelial phenotype through its effector HES1 and, consequently, loss of NOTCH activity favors the process of epithelial-mesenchymal transition. Evaluation of human bladder cancer samples revealed that tumors with low levels of HES1 present mesenchymal features and are more aggressive. Together, our results indicate that NOTCH serves as a tumor suppressor in the bladder and that loss of this pathway promotes mesenchymal and invasive features.

Authors

Antonio Maraver, Pablo J. Fernandez-Marcos, Timothy P. Cash, Marinela Mendez-Pertuz, Marta Dueñas, Paolo Maietta, Paola Martinelli, Maribel Muñoz-Martin, Mónica Martínez-Fernández, Marta Cañamero, Giovanna Roncador, Jorge L. Martinez-Torrecuadrada, Dimitrios Grivas, Jose Luis de la Pompa, Alfonso Valencia, Jesús M. Paramio, Francisco X. Real, Manuel Serrano

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Figure 1

NOTCH1 and NOTCH2 missense mutations found in human bladder carcinomas lead to decreased NOTCH activity.

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NOTCH1 and NOTCH2 missense mutations found in human bladder carcinomas l...
(A) T24 cells were cotransfected with a plasmid carrying a luciferase reporter gene directed by an RBPJ-responding promoter and with constructs carrying the indicated versions of NOTCH1 (N1) and NOTCH2 (N2). Subsequently, they were exposed to HEK293T cells overexpressing the NOTCH ligand JAGGED1 to activate NOTCH signaling and, consequently, luciferase expression. Bars represent the average of 5 independent measurements of luciferase, and error bars represent SD. The levels of overexpression were assessed by immunoblot (tubulin and FLAG immunoblots from the left panel were performed in two different but equivalent membranes; see complete unedited blots in the supplemental material). ***P < 0.001, Student’s t test. EV, empty vector. (B) Predicted structural effect of the NOTCH2-Y407C mutation is marked in red. Disulfide bridges predicted in WT and in NOTCH2-Y407C are marked in yellow.