First published March 1, 2013 - More info
TGF-β signaling can promote tumor formation and development or suppress it, depending on the cellular context and tumor stage. A potential target of this dual effect of TGF-β is HGF, as TGF-β can inhibit or promote its expression, although the mechanisms underlying this are largely unknown. In the present study, we found that mice with keratinocyte-specific deletion of the TGF-β signaling mediator Smad2 (referred to herein as K5.Smad2–/– mice), which have increased susceptibility to squamous cell carcinomas (SCCs), exhibited angiogenesis associated with epithelial overexpression of HGF and endothelial activation of the HGF receptor c-Met. Application of a c-Met inhibitor abrogated angiogenesis, suggesting that HGF overexpression plays a major role in angiogenesis associated with epithelial Smad2 loss. On the Hgf promoter, Smad2 was mainly associated with transcriptional corepressors, whereas Smad4 was mainly associated with the transcriptional coactivator CREB-binding protein (CBP/p300). Smad2 loss caused increased binding of Smad4 and CBP/p300 to the Hgf promoter. Consistent with this, knocking down Smad2 in human keratinocytes caused increased levels of HGF, which were abrogated by concomitant knockdown of Smad3 and Smad4. Importantly, the incidence of HGF-positive human SCC was high in cases with Smad2 loss and lower when Smad4 was also lost. We therefore conclude that Smad2 loss causes HGF upregulation via loss of Smad2-mediated transcriptional repression and enhanced Smad3/4-mediated transactivation. Since Smad2 is often downregulated in human SCCs, our data suggest a therapeutic strategy of blocking HGF/c-Met activation for Smad2-deficient SCCs.
Kristina E. Hoot, Masako Oka, Gangwen Han, Erwin Bottinger, Qinghong Zhang, Xiao-Jing Wang
Original citation: J. Clin. Invest. 2010;120(10):3606–3616. doi:10.1172/JCI43304.
Citation for this corrigendum: J. Clin. Invest. 2013;123(3):1402. doi:10.1172/JCI69077.
In the Methods section, the authors inadvertently provided incorrect quantitative PCR primer sequences for amplification of mouse and human HGF. The correct passage appears below.
HGF levels were determined using Power SYBR Green Master Mix (Applied Biosystems) and the following custom primers: 5ʹ-AGGAACAGGGGCTTTACGTT-3ʹ (forward) and 5ʹ-GTCAAATTCATGGCCAAACC -3ʹ (reverse). Human HGF quantitative PCR assays were performed using the TaqMan probe set Hs00300159_m1
The authors regret the error.