Ischemic liver CD45⁺ NPCs from CD11c-DTR mice treated with DT or PBS prior to 12 hours of I/R were cultured with Brefeldin A. (A) Intracellular IL-6 and TNF was determined 6 hours later. Numbers indicate the percentages within each gated region of cytokine staining in excess of isotype controls. More than 95% of the Ly6C^hi cells depicted are CD11b⁺ (data not shown). The bar graph depicts inflammatory monocyte cytokine production as MFI above isotype pooled from 3 experiments. (B) IL-10 receptor expression was measured on inflammatory monocytes, neutrophils, and Kupffer cells from ischemic livers of PBS-treated CD11c-DTR mice 12 hours after I/R or sham procedure. MFI values above isotype controls are shown and pooled from 3 experiments. Recipient CD11c-DTR mice pretreated with DT were injected with WT or Il10^–/– cDCs just prior to hepatic ischemia or sham procedure. (C) Twelve hours later, oxidative burst was measured (red line indicates sham treatment with WT cDCs; blue line indicates sham treatment with Il10^–/– cDCs; shaded area indicates I/R treatment with WT cDCs; bold line indicates I/R treatment with Il10^–/– cDCs). (D) Intracellular IL-6 and TNF production by inflammatory monocytes from liver CD45⁺ NPCs was measured and expressed as a percentage above isotype controls (black lines indicate isotype control; shaded area indicates sham treatment; bold lines indicate I/R treatment). The bar graphs in C and D depict data pooled from 3 experiments. (D) MFI values above isotype controls are shown only for I/R mice. Data represent mean ± SEM and are representative of at least 2 experiments; n = 4–6 mice per group. *P < 0.05; **P < 0.01.