(A) Mice (n = 5) were wounded by sterile incisions. Two days later, the mice were sacrificed and the RNA was extracted from the wounded skin surrounding the cut. The mRNA expression of the murine orthologs of NGAL (24p3) and SLPI was analyzed by real-time qRT-PCR and shown as the difference in threshold cycles (Ct) between the gene of interest and β-actin as housekeeping gene control. The expression of 24p3 and SLPI in nonwounded skin was set to 0. Thus, the bars at day 2 depict the induction of SLPI and 24p3 as shown in changes of threshold cycles (ΔδCt). (B) Mouse skin was sliced into 1 × 10–mm slices and incubated for 2 days in culture (n = 4). RNA was extracted from the skin. The expression of 24p3 and SLPI was analyzed by real-time qRT-PCR. The mRNA expression is shown as the difference in threshold cycles between the gene of interest and β-actin as housekeeping gene control (ΔδCt). The expression of 24p3 and SLPI in nonwounded skin was set to 0 so that the bars at day 2 depict the induction of SLPI and 24p3.