Go to JCI Insight
  • About
  • Editors
  • Consulting Editors
  • For authors
  • Alerts
  • Advertising/recruitment
  • Subscribe
  • Contact
  • Current Issue
  • Past Issues
  • By specialty
    • COVID-19
    • Cardiology
    • Gastroenterology
    • Immunology
    • Metabolism
    • Nephrology
    • Neuroscience
    • Oncology
    • Pulmonology
    • Vascular biology
    • All ...
  • Videos
    • Conversations with Giants in Medicine
    • Author's Takes
  • Reviews
    • View all reviews ...
    • 100th Anniversary of Insulin's Discovery (Jan 2021)
    • Hypoxia-inducible factors in disease pathophysiology and therapeutics (Oct 2020)
    • Latency in Infectious Disease (Jul 2020)
    • Immunotherapy in Hematological Cancers (Apr 2020)
    • Big Data's Future in Medicine (Feb 2020)
    • Mechanisms Underlying the Metabolic Syndrome (Oct 2019)
    • Reparative Immunology (Jul 2019)
    • View all review series ...
  • Viewpoint
  • Collections
    • Recently published
    • In-Press Preview
    • Commentaries
    • Concise Communication
    • Editorials
    • Viewpoint
    • Top read articles
  • Clinical Medicine
  • JCI This Month
    • Current issue
    • Past issues

  • Current issue
  • Past issues
  • Specialties
  • Reviews
  • Review series
  • Conversations with Giants in Medicine
  • Author's Takes
  • Recently published
  • In-Press Preview
  • Commentaries
  • Concise Communication
  • Editorials
  • Viewpoint
  • Top read articles
  • About
  • Editors
  • Consulting Editors
  • For authors
  • Alerts
  • Advertising/recruitment
  • Subscribe
  • Contact
ATP and purinergic receptor–dependent membrane traffic in bladder umbrella cells
Edward C.Y. Wang, … , Lori A. Birder, Gerard Apodaca
Edward C.Y. Wang, … , Lori A. Birder, Gerard Apodaca
Published September 1, 2005
Citation Information: J Clin Invest. 2005;115(9):2412-2422. https://doi.org/10.1172/JCI24086.
View: Text | PDF
Research Article Cell biology

ATP and purinergic receptor–dependent membrane traffic in bladder umbrella cells

  • Text
  • PDF
Abstract

The umbrella cells that line the bladder are mechanosensitive, and bladder filling increases the apical surface area of these cells; however, the upstream signals that regulate this process are unknown. Increased pressure stimulated ATP release from the isolated uroepithelium of rabbit bladders, which was blocked by inhibitors of vesicular transport, connexin hemichannels, ABC protein family members, and nucleoside transporters. Pressure-induced increases in membrane capacitance (a measure of apical plasma membrane surface area where 1 μF ≈ 1 cm2) were inhibited by the serosal, but not mucosal, addition of apyrase or the purinergic receptor antagonist PPADS. Upon addition of purinergic receptor agonists, increased capacitance was observed even in the absence of pressure. Moreover, knockout mice lacking expression of P2X2 and/or P2X3 receptors failed to show increases in apical surface area when exposed to hydrostatic pressure. Treatments that prevented release of Ca2+ from intracellular stores or activation of PKA blocked ATPγS-stimulated changes in capacitance. These results indicate that increased hydrostatic pressure stimulates release of ATP from the uroepithelium and that upon binding to P2X and possibly P2Y receptors on the umbrella cell, downstream Ca2+ and PKA second messenger cascades may act to stimulate membrane insertion at the apical pole of these cells.

Authors

Edward C.Y. Wang, Jey-Myung Lee, Wily G. Ruiz, Elena M. Balestreire, Maximilian von Bodungen, Stacey Barrick, Debra A. Cockayne, Lori A. Birder, Gerard Apodaca

×

Figure 6

Options: View larger image (or click on image) Download as PowerPoint
Transmission electron microscopy (TEM) analysis of apical pole of umbrel...
Transmission electron microscopy (TEM) analysis of apical pole of umbrella cells in wild-type and knockout mice. (A–F) C57BL/6J wild-type (A and D), P2X2_/_ (B and E), or P2X3_/_ (C and F) mice were catheterized, and the urine content of the bladder was drained. After a 30-minute incubation, the bladders were either excised and processed for TEM (A–C), or the bladder was slowly filled with normal saline at a rate of 1.4 μl/min for 180 minutes (D–F). Examples of fusiform vesicles (FV) are marked in A. (G–O) TEM analysis of the apical poles of umbrella cells from C57BL/6J wild-type (G, J, and M), P2X2_/_ (H, K, and N), or P2X3_/_ (I, L, and O) mice mounted in Ussing stretch chambers and incubated in the absence of hydrostatic pressure (G–I) or the presence of hydrostatic pressure (J–L) or treated with 10 μM forskolin (added to the mucosal hemichamber) in the absence of pressure (M–O).
Follow JCI:
Copyright © 2021 American Society for Clinical Investigation
ISSN: 0021-9738 (print), 1558-8238 (online)

Sign up for email alerts