Go to JCI Insight
  • About
  • Editors
  • Consulting Editors
  • For authors
  • Publication ethics
  • Publication alerts by email
  • Advertising
  • Job board
  • Contact
  • Clinical Research and Public Health
  • Current issue
  • Past issues
  • By specialty
    • COVID-19
    • Cardiology
    • Gastroenterology
    • Immunology
    • Metabolism
    • Nephrology
    • Neuroscience
    • Oncology
    • Pulmonology
    • Vascular biology
    • All ...
  • Videos
    • Conversations with Giants in Medicine
    • Video Abstracts
  • Reviews
    • View all reviews ...
    • Pancreatic Cancer (Jul 2025)
    • Complement Biology and Therapeutics (May 2025)
    • Evolving insights into MASLD and MASH pathogenesis and treatment (Apr 2025)
    • Microbiome in Health and Disease (Feb 2025)
    • Substance Use Disorders (Oct 2024)
    • Clonal Hematopoiesis (Oct 2024)
    • Sex Differences in Medicine (Sep 2024)
    • View all review series ...
  • Viewpoint
  • Collections
    • In-Press Preview
    • Clinical Research and Public Health
    • Research Letters
    • Letters to the Editor
    • Editorials
    • Commentaries
    • Editor's notes
    • Reviews
    • Viewpoints
    • 100th anniversary
    • Top read articles

  • Current issue
  • Past issues
  • Specialties
  • Reviews
  • Review series
  • Conversations with Giants in Medicine
  • Video Abstracts
  • In-Press Preview
  • Clinical Research and Public Health
  • Research Letters
  • Letters to the Editor
  • Editorials
  • Commentaries
  • Editor's notes
  • Reviews
  • Viewpoints
  • 100th anniversary
  • Top read articles
  • About
  • Editors
  • Consulting Editors
  • For authors
  • Publication ethics
  • Publication alerts by email
  • Advertising
  • Job board
  • Contact
Rab27a mediates the tight docking of insulin granules onto the plasma membrane during glucose stimulation
Kazuo Kasai, … , Hiroshi Gomi, Tetsuro Izumi
Kazuo Kasai, … , Hiroshi Gomi, Tetsuro Izumi
Published February 1, 2005
Citation Information: J Clin Invest. 2005;115(2):388-396. https://doi.org/10.1172/JCI22955.
View: Text | PDF
Article Cell biology

Rab27a mediates the tight docking of insulin granules onto the plasma membrane during glucose stimulation

  • Text
  • PDF
Abstract

The monomeric small GTPase Rab27a is specifically localized on both secretory granules and lysosome-related organelles. Although natural mutations of the Rab27a gene in human Griscelli syndrome and in ashen mice cause partial albinism and immunodeficiency reflecting the dysfunction of lysosome-related organelles, phenotypes resulting from the defective exocytosis of secretory granules have not been reported. To explore the roles of Rab27a in secretory granules, we analyzed insulin secretion profiles in ashen mice. Ashen mice showed glucose intolerance after a glucose load without signs of insulin resistance in peripheral tissues or insulin deficiency in the pancreas. Insulin secretion from isolated islets was decreased specifically in response to high glucose concentrations but not other nonphysiological secretagogues such as high K+ concentrations, forskolin, or phorbol ester. Neither the intracellular Ca2+ concentration nor the dynamics of fusion pore opening after glucose stimulation were altered. There were, however, marked reductions in the exocytosis from insulin granules predocked on the plasma membrane and in the replenishment of docked granules during glucose stimulation. These results provide the first genetic evidence to our knowledge for the role of Rab27a in the exocytosis of secretory granules and suggest that the Rab27a/effector system mediates glucose-specific signals for the exocytosis of insulin granules in pancreatic β cells.

Authors

Kazuo Kasai, Mica Ohara-Imaizumi, Noriko Takahashi, Shin Mizutani, Shengli Zhao, Toshiteru Kikuta, Haruo Kasai, Shinya Nagamatsu, Hiroshi Gomi, Tetsuro Izumi

×

Figure 5

Options: View larger image (or click on image) Download as PowerPoint
TIRFM analysis of the exocytosis of insulin granules. (A) Pancreatic β c...
TIRFM analysis of the exocytosis of insulin granules. (A) Pancreatic β cells from either C3H/He (left) or ashen (right) mice were fixed, immunostained with anti-insulin antibodies, and observed by TIRFM. The surrounding lines represent the outline of cells that are attached to the cover glass. Scale bar: 5 μm. (B) Pancreatic β cells of either C3H/He (upper) or ashen (lower) mice were infected with adenoviruses encoding insulin-GFP. Evanescent images in live cells were acquired every 300 milliseconds after glucose stimulation. The fusion events per 200 μm2 were manually counted. The histograms show the number of fusion events at 60-second intervals after high-glucose (16.7 mM) stimulation. The black bars show the fusion from previously docked granules, whereas the white bars represent that from newly recruited granules. Values are mean ± SE (n = 24 for ashen and n = 21 for C3H/He mice). The statistical significance of differences between means were assessed by repeated-measures ANOVA. (C) The number of morphologically docked granules was counted on the TIRFM images of pancreatic β cells that had been infected with adenoviruses encoding insulin-GFP. The number after a glucose stimulation (16.7 mM, 15 minutes) was normalized to that prior to the stimulation in the identical area (200 μm2) of C3H/He (open circles) or ashen (filled circles) β cells. Values are mean ± SE (n = 4). The statistical significance of differences between means was assessed by Student’s t test. *P < 0.0005 vs. C3H/He mice.

Copyright © 2025 American Society for Clinical Investigation
ISSN: 0021-9738 (print), 1558-8238 (online)

Sign up for email alerts