(A–D) Blood ionized calcium alterations in (A) Casr^+/+ and (B) Casr^+/Nuf mice treated with vehicle and in (C) Casr^+/+ and (D) Casr^+/Nuf mice treated with 2 nmol/kg EPT. (E–L) Effect of 1–4 nmol/kg EPT on plasma concentrations of (E) adjusted calcium (Adj-calcium), (F) phosphate, (G) magnesium, (H) calcium x phosphate, (I) 1,25(OH)₂D, (J) ALP, (K) P1NP, and (L) CTX-1. (M–P) Association of adjusted calcium with (M) 1,25(OH)₂D, (N) ALP, (O) P1NP, and (P) CTX-1. (Q–S) FE of (Q) calcium (FECa), (R) magnesium (FEMg), and (S) phosphate (FEPi). (T) BMD of male and female Casr^+/Nuf mice treated with vehicle (–) or 2 nmol/kg EPT (+). Mean ± SEM values are shown in the bar charts and scatter plots. Shaded and open bars represent Casr^+/+ and Casr^+/Nuf mice, respectively. Squares denote male mice; circles denote female mice. *P < 0.05, **P < 0.01, and ***P < 0.001. Two-group comparisons were assessed by 2-tailed Student’s t test and comparison of 3 or more groups by 1-way ANOVA.