(A and B) Quantification of specific 5′-tRNA halves (A) and Ang mRNA (B) in OE cells. Relative expression levels are shown with one control sample set as 1. After outlier exclusion (see Statistics in Supplemental Methods), sample sizes were 7 for 5′-GlyGCC, 5′-HisGUG, and 5′-ValCAC/AAC, and 8 for 5′-LysCUU and Ang mRNA. P values were determined using a paired 2-tailed t test. (C) Average proportions of tRNA-derived sncRNA reads obtained by cP-RNA-Seq. (D) Pearson’s correlation analysis among tRNA-derived sncRNA subclasses. The color and size of dots reflect the correlation coefficient, calculated as the z score. (E) Heatmap of 5′-tRNA half read distributions. Coloration reflects the log₁₀ reads per million–based z score. (F) Average proportions of 5′-derivative reads across all samples. (G) Analysis of read positions for each 5′-derivative. The highest read count within each subclass was set to 100%, and the counts at each nucleotide position are shown as relative abundances with SD from all samples shown as shaded regions.