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Maturational differences in lung NF-κB activation and their role in tolerance to hyperoxia
Guang Yang, … , Yi-Hao Weng, Phyllis A. Dennery
Guang Yang, … , Yi-Hao Weng, Phyllis A. Dennery
Published September 1, 2004
Citation Information: J Clin Invest. 2004;114(5):669-678. https://doi.org/10.1172/JCI19300.
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Article Inflammation

Maturational differences in lung NF-κB activation and their role in tolerance to hyperoxia

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Abstract

Neonatal rodents are more tolerant to hyperoxia than adults. We determined whether maturational differences in lung NF-κB activation could account for the differences. After hyperoxic exposure (O2 > 95%), neonatal (<12 hours old) lung NF-κB binding was increased and reached a maximum between 8 and 16 hours, whereas in adults no changes were observed. Additionally, neonatal NF-κB/luciferase transgenic mice (incorporating 2 NF-κB consensus sequences driving luciferase gene expression) demonstrated enhanced in vivo NF-κB activation after hyperoxia in real time. In the lungs of neonates, there was a propensity toward NF-κB activation as evidenced by increased lung I-κB kinase protein levels, I-κBα phosphorylation, β-transducin repeat–containing protein levels, and total I-κBα degradation. Increased lung p-JNK immunoreactive protein was observed only in the adult lung. Inhibition of pI-κBα by BAY 11-7085 resulted in decreased Bcl-2 protein levels in neonatal lung homogenates and decreased cell viability in lung primary cultures after hyperoxic exposure. Furthermore, neonatal p50-null mutant (p50–/–) mice showed increased lung DNA degradation and decreased survival in hyperoxia compared with WT mice. These data demonstrate that there are maturational differences in lung NF-κB activation and that enhanced NF-κB may serve to protect the neonatal lung from acute hyperoxic injury via inhibition of apoptosis.

Authors

Guang Yang, Aida Abate, Adia G. George, Yi-Hao Weng, Phyllis A. Dennery

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Figure 3

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Maturational differences in pI-κBα, total I-κBα, and β-TrCP in hyperoxia...
Maturational differences in pI-κBα, total I-κBα, and β-TrCP in hyperoxia. (A) Expression of pI-κBα at early time points. 0–4 hours, duration of hyperoxia. (B) Expression of I-κBα from 0 to 72 hours of hyperoxia. (C) Densitometric evaluation of I-κBα protein levels from the blot shown in B. (D) Relative mobility of the I-κBα bands after hyperoxia as compared with that in cell extracts with (T) or without (U) TNF-α treatment. Note that the lower band of the I-κBα represents pI-κBα. (E) β-TrCP protein levels after hyperoxia in the nuclear fractions of the mouse lung.
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