(A) Survival curves after infection with M. tuberculosis (n = 7–10) and L. monocytogenes (n = 4–5). Spleen CFU on day 21 after S. typhimurium infection (n = 4–9). (B) Liver on day 3 after L. monocytogenes infection stained for TUNEL and DAPI. Scale bar: 200 μm. (C) Quantification of TUNEL⁺ foci (n = 3). (D) Peritoneal lavage CFU at 4 hours, day 1, and day 3 after L. monocytogenes (n = 4–8). (E) Isolated F4/80^hi peritoneal macrophages exposed to L. monocytogenes were permeabilized for CFU count after 24 hours. (F) Macrophages were stained for L. monocytogenes and lysosome (LAMP1) at 6 hours and quantified for volumetric pixels of L. monocytogenes that were LAMP1⁺ (n = 32 images). (G) Percentage GFP⁺ after 4-hour infection by GFP-tagged L. monocytogenes (n = 9–10). (H) Neutrophil and (I) F4/80^hi tissue macrophage numbers in infected peritoneal lavage (n = 7–11). (J) Representative plot showing depletion of CD11b⁺F4/80^hi macrophages at 24 hours. (K) CFU in peritoneal cavity (PerC), liver, and spleen on day 3 after L. monocytogenes (n = 5–8). Data are shown as the mean ± SEM. ^#P < 0.08, *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001 (1-way ANOVA with Tukey’s adjustment for A and C–I or Student’s t test for K).