(A) WT mice were intraperitoneally injected with anti–IL-6R or control IgG on immunization days –1, 3, 7, 11, and 15(n = 5 mice/group). After EAE induction, mice were treated with rhCTRP4 or BSA daily from day 0 to day 27. Mean clinical scores show progression of disease. (B) CNS monocytes were harvested on day 18 and quantified as absolute cell numbers of indicated CNS-infiltrating cell populations gated on CD45⁺. (C) CNS monocytes were harvested on day 18 and quantified as absolute numbers of CD4⁺IFN-γ⁺, CD4⁺IL-17A⁺, and CD4⁺IL-17A⁺IFN-γ⁺ in CNS after stimulating with PMA and inomycin with GolgiPlug for 5 hours. (D) Ctrp4^–/– and WT mice subjected to MOG-induced EAE were treated with selective STAT3 inhibitor S3I-201 (10 mg/kg/d dissolved in 20%DMSO/80% corn oil). Control mice were injected with equal volumes of vehicle. Each group was monitored and scored daily (n = 7/group). (E) Representative images of H&E staining and LFB staining of spinal cord sections show inflammatory cell infiltration and demyelination, respectively. Scale bar: 200 μm. Data are represented as mean ± SEM and were analyzed by 1-way ANOVA with Tukey’s post test (B and C). Two-way repeated measures ANOVA and Holm-Šidák post hoc test (A and D).*P < 0.05; **P < 0.01.