(A) Mean clinical scores of chimeric mice generated by (i) transfer of WT bone marrow cells into irradiated WT and Ctrp4^–/– recipient mice or (ii) transfer of Ctrp4^–/– bone marrow cells into irradiated WT and Ctrp4^–/– recipient mice (each n = 6–8) following MOG_35–55 immunization. Statistical significance was determined using 2-way repeated measures ANOVA and Holm-Šidák post hoc test. (B) Representative H&E staining of spinal cord sections harvested from chimeric WT and Ctrp4^–/– mice showed inflammatory cell infiltration and demyelination at day 18 after immunization. Scale bars: 200 μm. (C) Flow-cytometric analysis of absolute cell numbers of CNS-infiltrating T cells (CD45⁺CD3⁺CD4⁺ T and CD45⁺CD3⁺CD8⁺ T) and B cells (CD45⁺CD3^–CD19⁺) at 18 days after immunization. (D) Flow-cytometric analysis of absolute numbers of different CNS-infiltrating myeloid cells including monocytes (CD45⁺CD11b⁺Ly6C⁺), neutrophils (CD45⁺CD11b⁺Ly6G⁺), and DCs (CD45⁺CD11c⁺MHCII⁺) at 18 days after immunization. (E) Flow-cytometric analysis of absolute numbers of Th1 cells (IFN-γ⁺), Th17 cells (IL-17A⁺), and Tregs (CD25⁺Foxp3⁺) of CD4⁺ T cells infiltrated to the CNS harvested at 18 days after immunization. (F) Mean clinical scores of CTRP4^fl/fl and CD4-cre CTRP4^fl/fl mice were monitored after MOG immunization. Data are representative of 3 independent experiments. Statistical significance was determined using 2-way repeated-measures ANOVA. (G) H&E staining (left) and LFB staining (right) of spinal cord sections harvested from CTRP4^fl/fl and CD4-cre CTRP4^fl/fl mice at day 18 after EAE induction. Data are represented as mean ± SEM and are from 1 of 3 independent experiments with similar results. One-way ANOVA with Tukey’s post test (C–E). *P < 0.05; **P < 0.01; ***P < 0.0001.