C5a anaphylatoxin is a major regulator of activating versus inhibitory FcγRs in immune complex–induced lung disease
Nelli Shushakova, Julia Skokowa, Jurriaan Schulman, Ulrich Baumann, Jörg Zwirner, Reinhold E. Schmidt, J. Engelbert Gessner
Nelli Shushakova, Julia Skokowa, Jurriaan Schulman, Ulrich Baumann, Jörg Zwirner, Reinhold E. Schmidt, J. Engelbert Gessner
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Article Autoimmunity

C5a anaphylatoxin is a major regulator of activating versus inhibitory FcγRs in immune complex–induced lung disease

Abstract

Research Article

Authors

Nelli Shushakova, Julia Skokowa, Jurriaan Schulman, Ulrich Baumann, Jörg Zwirner, Reinhold E. Schmidt, J. Engelbert Gessner

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Flow cytometric detection of C5aR and FcγRII/III on AMs. (a) Control cel...
Flow cytometric detection of C5aR and FcγRII/III on AMs. (a) Control cells from peripheral blood (PBC) and resident AMs isolated from BAL fluid of C57BL/6 WT and C5aR–/– mice were stained with the newly developed anti-C5aR mAb 20/70 conjugated to FITC in combination with PE anti-FcγRII/III 2.4G2 mAb and analyzed on a FACScan. (b) MH-S AM cells were cultured under 10% FCS medium conditions. Simultaneous expression of FcγRII/III and C5aR was detected by FACS analysis using PE-2.4G2 and FITC-20/70 mAbs.