Anti-CCL25 antibody reduces OT-1 cell recruitment to the small-intestinal epithelium. OT-1 (Ly 5.2+) cells were injected into C57BL/6J-Ly5.1 mice, and the percentage of CD8β+ lymphocytes expressing Ly5.2 in the MLNs (a), PLNs (b), liver (c), and small intestine (d) of control or anti-CCL25 antibody–treated mice was determined by flow cytometry 3 days after intraperitoneal challenge with OVA. Results are the mean ± SEM of six (PLN, MLN, liver) and four (small intestine) mice in each group and show one representative experiment of three performed. *P < 0.05. (e) Representative flow cytometry analysis of the small-intestinal IEL populations in each group of mice.