Effect of different NSAIDs on MMP-2 activation, MT1-MMP expression, and tumor cell invasion induced by HBx. (a) CMO and CMX cells were grown for 24 hours in the presence of 60 μg/ml indomethacin, 60 μg/ml meloxicam, 600 μg/ml ASA, or DMSO as a control, and the amount of activated MMP-2 and MT1-MMP was analyzed by Western blot. (b) CMX and CMO cells were allowed to invade a Matrigel-coated Transwell in the presence of 60 μg/ml indomethacin, 60 μg/ml meloxicam, 600 μg/ml ASA, or DMSO. Cells that migrated to the lower chamber were quantified as in Figure 1.