Roshni R. Singaraja, Catherine Fievet, Graciela Castro, Erick R. James, Nathalie Hennuyer, Susanne M. Clee, Nagat Bissada, Jonathan C. Choy, Jean-Charles Fruchart, Bruce M. McManus, Bart Staels, Michael R. Hayden
Analysis of efflux levels in primary macrophages from BAC+ApoE–/– mice. Primary peritoneal macrophage cultures were established from the BAC+ApoE–/– and control mice as described (n = 4) (17). A 38.4% increase (P < 0.001) in efflux in the BAC+ApoE–/– macrophages is evident when compared with the ApoE–/– littermates. Upon stimulation of the cultures with 9(cis)-retinoic acid and 22(R)-hydroxy cholesterol, there was a 23.7% increase (P < 0.001) in cholesterol efflux between BAC+ApoE–/– versus control littermates. There was a further 46.8% increase (P < 0.001) in efflux in the BAC+ApoE–/– macrophages after stimulation with 9(cis)-retinoic acid and 22(R)-hydroxy cholesterol when compared with unstimulated cells.