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Clinical MedicineIn-Press PreviewInfectious diseaseVirology Open Access | 10.1172/JCI157415

Chemerin and galectin-3-binding protein are prognostic amniotic fluid biomarkers for congenital cytomegalovirus infection severity

Olesya Vorontsov,1 Lorinne Levitt,2 Daniele Lilleri,3 Gilad W. Vainer,4 Orit Caplan,5 Licita Schreiber,6 Alessia Arossa,7 Arsenio Spinillo,7 Milena Furione,3 Or Alfi,1 Esther Oiknine-Djian,5 Meital Kupervaser,8 Yuval Nevo,9 Sharona Elgavish,9 Moran Yassour,10 Maurizio Zavattoni,3 Tali Bdolah-Abram,11 Fausto Baldanti,3 Miriam Geal-Dor,12 Zichria Zakay-Rones,13 Nili Yanai,2 Simcha Yagel,2 Amos Panet,13 and Dana G. Wolf5

1Clinical Virology Unit, The Hebrew University of Jerusalem, Jerusalem, Israel

2Department of Obstetrics and Gynecology, Hadassah Hebrew University Medical Center, Jerusalem, Israel

3Department of Microbiology and Virology, IRCCS Policlinico San Matteo Foundation and University of Pavia, Pavia, Italy

4Department of Pathology, Hadassah Hebrew University Medical Center, Jerusalem, Israel

5Clinical Virology Unit, Hadassah Hebrew University Medical Center, Jerusalem, Israel

6Maccabi Healthcare Services, Rehovot, Israel

7Department of Obstetrics and Gynecology, IRCCS Policlinico San Matteo Foundation and University of Pavia, Pavia, Italy

8Grand Israel National Center for Personalized Medicine, Weizmann Institute of Science, Rehovot, Israel

9Info-CORE, Bioinformatics Unit of the I-CORE, The Hebrew University of Jerusalem, Jerusalem, Israel

10School of Computer Science and Engineering, The Hebrew University of Jerusalem, Jerusalem, Israel

11Faculty of Medicine, The Hebrew University of Jerusalem, Jerusalem, Israel

12Department of Speech and Hearing, Hadassah Hebrew University Medical Center, Jerusalem, Israel

13Department of Biochemistry, The Hebrew University of Jerusalem, Jerusalem, Israel

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1Clinical Virology Unit, The Hebrew University of Jerusalem, Jerusalem, Israel

2Department of Obstetrics and Gynecology, Hadassah Hebrew University Medical Center, Jerusalem, Israel

3Department of Microbiology and Virology, IRCCS Policlinico San Matteo Foundation and University of Pavia, Pavia, Italy

4Department of Pathology, Hadassah Hebrew University Medical Center, Jerusalem, Israel

5Clinical Virology Unit, Hadassah Hebrew University Medical Center, Jerusalem, Israel

6Maccabi Healthcare Services, Rehovot, Israel

7Department of Obstetrics and Gynecology, IRCCS Policlinico San Matteo Foundation and University of Pavia, Pavia, Italy

8Grand Israel National Center for Personalized Medicine, Weizmann Institute of Science, Rehovot, Israel

9Info-CORE, Bioinformatics Unit of the I-CORE, The Hebrew University of Jerusalem, Jerusalem, Israel

10School of Computer Science and Engineering, The Hebrew University of Jerusalem, Jerusalem, Israel

11Faculty of Medicine, The Hebrew University of Jerusalem, Jerusalem, Israel

12Department of Speech and Hearing, Hadassah Hebrew University Medical Center, Jerusalem, Israel

13Department of Biochemistry, The Hebrew University of Jerusalem, Jerusalem, Israel

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1Clinical Virology Unit, The Hebrew University of Jerusalem, Jerusalem, Israel

2Department of Obstetrics and Gynecology, Hadassah Hebrew University Medical Center, Jerusalem, Israel

3Department of Microbiology and Virology, IRCCS Policlinico San Matteo Foundation and University of Pavia, Pavia, Italy

4Department of Pathology, Hadassah Hebrew University Medical Center, Jerusalem, Israel

5Clinical Virology Unit, Hadassah Hebrew University Medical Center, Jerusalem, Israel

6Maccabi Healthcare Services, Rehovot, Israel

7Department of Obstetrics and Gynecology, IRCCS Policlinico San Matteo Foundation and University of Pavia, Pavia, Italy

8Grand Israel National Center for Personalized Medicine, Weizmann Institute of Science, Rehovot, Israel

9Info-CORE, Bioinformatics Unit of the I-CORE, The Hebrew University of Jerusalem, Jerusalem, Israel

10School of Computer Science and Engineering, The Hebrew University of Jerusalem, Jerusalem, Israel

11Faculty of Medicine, The Hebrew University of Jerusalem, Jerusalem, Israel

12Department of Speech and Hearing, Hadassah Hebrew University Medical Center, Jerusalem, Israel

13Department of Biochemistry, The Hebrew University of Jerusalem, Jerusalem, Israel

Find articles by Lilleri, D. in: JCI | PubMed | Google Scholar |

1Clinical Virology Unit, The Hebrew University of Jerusalem, Jerusalem, Israel

2Department of Obstetrics and Gynecology, Hadassah Hebrew University Medical Center, Jerusalem, Israel

3Department of Microbiology and Virology, IRCCS Policlinico San Matteo Foundation and University of Pavia, Pavia, Italy

4Department of Pathology, Hadassah Hebrew University Medical Center, Jerusalem, Israel

5Clinical Virology Unit, Hadassah Hebrew University Medical Center, Jerusalem, Israel

6Maccabi Healthcare Services, Rehovot, Israel

7Department of Obstetrics and Gynecology, IRCCS Policlinico San Matteo Foundation and University of Pavia, Pavia, Italy

8Grand Israel National Center for Personalized Medicine, Weizmann Institute of Science, Rehovot, Israel

9Info-CORE, Bioinformatics Unit of the I-CORE, The Hebrew University of Jerusalem, Jerusalem, Israel

10School of Computer Science and Engineering, The Hebrew University of Jerusalem, Jerusalem, Israel

11Faculty of Medicine, The Hebrew University of Jerusalem, Jerusalem, Israel

12Department of Speech and Hearing, Hadassah Hebrew University Medical Center, Jerusalem, Israel

13Department of Biochemistry, The Hebrew University of Jerusalem, Jerusalem, Israel

Find articles by Vainer, G. in: JCI | PubMed | Google Scholar

1Clinical Virology Unit, The Hebrew University of Jerusalem, Jerusalem, Israel

2Department of Obstetrics and Gynecology, Hadassah Hebrew University Medical Center, Jerusalem, Israel

3Department of Microbiology and Virology, IRCCS Policlinico San Matteo Foundation and University of Pavia, Pavia, Italy

4Department of Pathology, Hadassah Hebrew University Medical Center, Jerusalem, Israel

5Clinical Virology Unit, Hadassah Hebrew University Medical Center, Jerusalem, Israel

6Maccabi Healthcare Services, Rehovot, Israel

7Department of Obstetrics and Gynecology, IRCCS Policlinico San Matteo Foundation and University of Pavia, Pavia, Italy

8Grand Israel National Center for Personalized Medicine, Weizmann Institute of Science, Rehovot, Israel

9Info-CORE, Bioinformatics Unit of the I-CORE, The Hebrew University of Jerusalem, Jerusalem, Israel

10School of Computer Science and Engineering, The Hebrew University of Jerusalem, Jerusalem, Israel

11Faculty of Medicine, The Hebrew University of Jerusalem, Jerusalem, Israel

12Department of Speech and Hearing, Hadassah Hebrew University Medical Center, Jerusalem, Israel

13Department of Biochemistry, The Hebrew University of Jerusalem, Jerusalem, Israel

Find articles by Caplan, O. in: JCI | PubMed | Google Scholar

1Clinical Virology Unit, The Hebrew University of Jerusalem, Jerusalem, Israel

2Department of Obstetrics and Gynecology, Hadassah Hebrew University Medical Center, Jerusalem, Israel

3Department of Microbiology and Virology, IRCCS Policlinico San Matteo Foundation and University of Pavia, Pavia, Italy

4Department of Pathology, Hadassah Hebrew University Medical Center, Jerusalem, Israel

5Clinical Virology Unit, Hadassah Hebrew University Medical Center, Jerusalem, Israel

6Maccabi Healthcare Services, Rehovot, Israel

7Department of Obstetrics and Gynecology, IRCCS Policlinico San Matteo Foundation and University of Pavia, Pavia, Italy

8Grand Israel National Center for Personalized Medicine, Weizmann Institute of Science, Rehovot, Israel

9Info-CORE, Bioinformatics Unit of the I-CORE, The Hebrew University of Jerusalem, Jerusalem, Israel

10School of Computer Science and Engineering, The Hebrew University of Jerusalem, Jerusalem, Israel

11Faculty of Medicine, The Hebrew University of Jerusalem, Jerusalem, Israel

12Department of Speech and Hearing, Hadassah Hebrew University Medical Center, Jerusalem, Israel

13Department of Biochemistry, The Hebrew University of Jerusalem, Jerusalem, Israel

Find articles by Schreiber, L. in: JCI | PubMed | Google Scholar

1Clinical Virology Unit, The Hebrew University of Jerusalem, Jerusalem, Israel

2Department of Obstetrics and Gynecology, Hadassah Hebrew University Medical Center, Jerusalem, Israel

3Department of Microbiology and Virology, IRCCS Policlinico San Matteo Foundation and University of Pavia, Pavia, Italy

4Department of Pathology, Hadassah Hebrew University Medical Center, Jerusalem, Israel

5Clinical Virology Unit, Hadassah Hebrew University Medical Center, Jerusalem, Israel

6Maccabi Healthcare Services, Rehovot, Israel

7Department of Obstetrics and Gynecology, IRCCS Policlinico San Matteo Foundation and University of Pavia, Pavia, Italy

8Grand Israel National Center for Personalized Medicine, Weizmann Institute of Science, Rehovot, Israel

9Info-CORE, Bioinformatics Unit of the I-CORE, The Hebrew University of Jerusalem, Jerusalem, Israel

10School of Computer Science and Engineering, The Hebrew University of Jerusalem, Jerusalem, Israel

11Faculty of Medicine, The Hebrew University of Jerusalem, Jerusalem, Israel

12Department of Speech and Hearing, Hadassah Hebrew University Medical Center, Jerusalem, Israel

13Department of Biochemistry, The Hebrew University of Jerusalem, Jerusalem, Israel

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1Clinical Virology Unit, The Hebrew University of Jerusalem, Jerusalem, Israel

2Department of Obstetrics and Gynecology, Hadassah Hebrew University Medical Center, Jerusalem, Israel

3Department of Microbiology and Virology, IRCCS Policlinico San Matteo Foundation and University of Pavia, Pavia, Italy

4Department of Pathology, Hadassah Hebrew University Medical Center, Jerusalem, Israel

5Clinical Virology Unit, Hadassah Hebrew University Medical Center, Jerusalem, Israel

6Maccabi Healthcare Services, Rehovot, Israel

7Department of Obstetrics and Gynecology, IRCCS Policlinico San Matteo Foundation and University of Pavia, Pavia, Italy

8Grand Israel National Center for Personalized Medicine, Weizmann Institute of Science, Rehovot, Israel

9Info-CORE, Bioinformatics Unit of the I-CORE, The Hebrew University of Jerusalem, Jerusalem, Israel

10School of Computer Science and Engineering, The Hebrew University of Jerusalem, Jerusalem, Israel

11Faculty of Medicine, The Hebrew University of Jerusalem, Jerusalem, Israel

12Department of Speech and Hearing, Hadassah Hebrew University Medical Center, Jerusalem, Israel

13Department of Biochemistry, The Hebrew University of Jerusalem, Jerusalem, Israel

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1Clinical Virology Unit, The Hebrew University of Jerusalem, Jerusalem, Israel

2Department of Obstetrics and Gynecology, Hadassah Hebrew University Medical Center, Jerusalem, Israel

3Department of Microbiology and Virology, IRCCS Policlinico San Matteo Foundation and University of Pavia, Pavia, Italy

4Department of Pathology, Hadassah Hebrew University Medical Center, Jerusalem, Israel

5Clinical Virology Unit, Hadassah Hebrew University Medical Center, Jerusalem, Israel

6Maccabi Healthcare Services, Rehovot, Israel

7Department of Obstetrics and Gynecology, IRCCS Policlinico San Matteo Foundation and University of Pavia, Pavia, Italy

8Grand Israel National Center for Personalized Medicine, Weizmann Institute of Science, Rehovot, Israel

9Info-CORE, Bioinformatics Unit of the I-CORE, The Hebrew University of Jerusalem, Jerusalem, Israel

10School of Computer Science and Engineering, The Hebrew University of Jerusalem, Jerusalem, Israel

11Faculty of Medicine, The Hebrew University of Jerusalem, Jerusalem, Israel

12Department of Speech and Hearing, Hadassah Hebrew University Medical Center, Jerusalem, Israel

13Department of Biochemistry, The Hebrew University of Jerusalem, Jerusalem, Israel

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1Clinical Virology Unit, The Hebrew University of Jerusalem, Jerusalem, Israel

2Department of Obstetrics and Gynecology, Hadassah Hebrew University Medical Center, Jerusalem, Israel

3Department of Microbiology and Virology, IRCCS Policlinico San Matteo Foundation and University of Pavia, Pavia, Italy

4Department of Pathology, Hadassah Hebrew University Medical Center, Jerusalem, Israel

5Clinical Virology Unit, Hadassah Hebrew University Medical Center, Jerusalem, Israel

6Maccabi Healthcare Services, Rehovot, Israel

7Department of Obstetrics and Gynecology, IRCCS Policlinico San Matteo Foundation and University of Pavia, Pavia, Italy

8Grand Israel National Center for Personalized Medicine, Weizmann Institute of Science, Rehovot, Israel

9Info-CORE, Bioinformatics Unit of the I-CORE, The Hebrew University of Jerusalem, Jerusalem, Israel

10School of Computer Science and Engineering, The Hebrew University of Jerusalem, Jerusalem, Israel

11Faculty of Medicine, The Hebrew University of Jerusalem, Jerusalem, Israel

12Department of Speech and Hearing, Hadassah Hebrew University Medical Center, Jerusalem, Israel

13Department of Biochemistry, The Hebrew University of Jerusalem, Jerusalem, Israel

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1Clinical Virology Unit, The Hebrew University of Jerusalem, Jerusalem, Israel

2Department of Obstetrics and Gynecology, Hadassah Hebrew University Medical Center, Jerusalem, Israel

3Department of Microbiology and Virology, IRCCS Policlinico San Matteo Foundation and University of Pavia, Pavia, Italy

4Department of Pathology, Hadassah Hebrew University Medical Center, Jerusalem, Israel

5Clinical Virology Unit, Hadassah Hebrew University Medical Center, Jerusalem, Israel

6Maccabi Healthcare Services, Rehovot, Israel

7Department of Obstetrics and Gynecology, IRCCS Policlinico San Matteo Foundation and University of Pavia, Pavia, Italy

8Grand Israel National Center for Personalized Medicine, Weizmann Institute of Science, Rehovot, Israel

9Info-CORE, Bioinformatics Unit of the I-CORE, The Hebrew University of Jerusalem, Jerusalem, Israel

10School of Computer Science and Engineering, The Hebrew University of Jerusalem, Jerusalem, Israel

11Faculty of Medicine, The Hebrew University of Jerusalem, Jerusalem, Israel

12Department of Speech and Hearing, Hadassah Hebrew University Medical Center, Jerusalem, Israel

13Department of Biochemistry, The Hebrew University of Jerusalem, Jerusalem, Israel

Find articles by Oiknine-Djian, E. in: JCI | PubMed | Google Scholar

1Clinical Virology Unit, The Hebrew University of Jerusalem, Jerusalem, Israel

2Department of Obstetrics and Gynecology, Hadassah Hebrew University Medical Center, Jerusalem, Israel

3Department of Microbiology and Virology, IRCCS Policlinico San Matteo Foundation and University of Pavia, Pavia, Italy

4Department of Pathology, Hadassah Hebrew University Medical Center, Jerusalem, Israel

5Clinical Virology Unit, Hadassah Hebrew University Medical Center, Jerusalem, Israel

6Maccabi Healthcare Services, Rehovot, Israel

7Department of Obstetrics and Gynecology, IRCCS Policlinico San Matteo Foundation and University of Pavia, Pavia, Italy

8Grand Israel National Center for Personalized Medicine, Weizmann Institute of Science, Rehovot, Israel

9Info-CORE, Bioinformatics Unit of the I-CORE, The Hebrew University of Jerusalem, Jerusalem, Israel

10School of Computer Science and Engineering, The Hebrew University of Jerusalem, Jerusalem, Israel

11Faculty of Medicine, The Hebrew University of Jerusalem, Jerusalem, Israel

12Department of Speech and Hearing, Hadassah Hebrew University Medical Center, Jerusalem, Israel

13Department of Biochemistry, The Hebrew University of Jerusalem, Jerusalem, Israel

Find articles by Kupervaser, M. in: JCI | PubMed | Google Scholar |

1Clinical Virology Unit, The Hebrew University of Jerusalem, Jerusalem, Israel

2Department of Obstetrics and Gynecology, Hadassah Hebrew University Medical Center, Jerusalem, Israel

3Department of Microbiology and Virology, IRCCS Policlinico San Matteo Foundation and University of Pavia, Pavia, Italy

4Department of Pathology, Hadassah Hebrew University Medical Center, Jerusalem, Israel

5Clinical Virology Unit, Hadassah Hebrew University Medical Center, Jerusalem, Israel

6Maccabi Healthcare Services, Rehovot, Israel

7Department of Obstetrics and Gynecology, IRCCS Policlinico San Matteo Foundation and University of Pavia, Pavia, Italy

8Grand Israel National Center for Personalized Medicine, Weizmann Institute of Science, Rehovot, Israel

9Info-CORE, Bioinformatics Unit of the I-CORE, The Hebrew University of Jerusalem, Jerusalem, Israel

10School of Computer Science and Engineering, The Hebrew University of Jerusalem, Jerusalem, Israel

11Faculty of Medicine, The Hebrew University of Jerusalem, Jerusalem, Israel

12Department of Speech and Hearing, Hadassah Hebrew University Medical Center, Jerusalem, Israel

13Department of Biochemistry, The Hebrew University of Jerusalem, Jerusalem, Israel

Find articles by Nevo, Y. in: JCI | PubMed | Google Scholar

1Clinical Virology Unit, The Hebrew University of Jerusalem, Jerusalem, Israel

2Department of Obstetrics and Gynecology, Hadassah Hebrew University Medical Center, Jerusalem, Israel

3Department of Microbiology and Virology, IRCCS Policlinico San Matteo Foundation and University of Pavia, Pavia, Italy

4Department of Pathology, Hadassah Hebrew University Medical Center, Jerusalem, Israel

5Clinical Virology Unit, Hadassah Hebrew University Medical Center, Jerusalem, Israel

6Maccabi Healthcare Services, Rehovot, Israel

7Department of Obstetrics and Gynecology, IRCCS Policlinico San Matteo Foundation and University of Pavia, Pavia, Italy

8Grand Israel National Center for Personalized Medicine, Weizmann Institute of Science, Rehovot, Israel

9Info-CORE, Bioinformatics Unit of the I-CORE, The Hebrew University of Jerusalem, Jerusalem, Israel

10School of Computer Science and Engineering, The Hebrew University of Jerusalem, Jerusalem, Israel

11Faculty of Medicine, The Hebrew University of Jerusalem, Jerusalem, Israel

12Department of Speech and Hearing, Hadassah Hebrew University Medical Center, Jerusalem, Israel

13Department of Biochemistry, The Hebrew University of Jerusalem, Jerusalem, Israel

Find articles by Elgavish, S. in: JCI | PubMed | Google Scholar

1Clinical Virology Unit, The Hebrew University of Jerusalem, Jerusalem, Israel

2Department of Obstetrics and Gynecology, Hadassah Hebrew University Medical Center, Jerusalem, Israel

3Department of Microbiology and Virology, IRCCS Policlinico San Matteo Foundation and University of Pavia, Pavia, Italy

4Department of Pathology, Hadassah Hebrew University Medical Center, Jerusalem, Israel

5Clinical Virology Unit, Hadassah Hebrew University Medical Center, Jerusalem, Israel

6Maccabi Healthcare Services, Rehovot, Israel

7Department of Obstetrics and Gynecology, IRCCS Policlinico San Matteo Foundation and University of Pavia, Pavia, Italy

8Grand Israel National Center for Personalized Medicine, Weizmann Institute of Science, Rehovot, Israel

9Info-CORE, Bioinformatics Unit of the I-CORE, The Hebrew University of Jerusalem, Jerusalem, Israel

10School of Computer Science and Engineering, The Hebrew University of Jerusalem, Jerusalem, Israel

11Faculty of Medicine, The Hebrew University of Jerusalem, Jerusalem, Israel

12Department of Speech and Hearing, Hadassah Hebrew University Medical Center, Jerusalem, Israel

13Department of Biochemistry, The Hebrew University of Jerusalem, Jerusalem, Israel

Find articles by Yassour, M. in: JCI | PubMed | Google Scholar

1Clinical Virology Unit, The Hebrew University of Jerusalem, Jerusalem, Israel

2Department of Obstetrics and Gynecology, Hadassah Hebrew University Medical Center, Jerusalem, Israel

3Department of Microbiology and Virology, IRCCS Policlinico San Matteo Foundation and University of Pavia, Pavia, Italy

4Department of Pathology, Hadassah Hebrew University Medical Center, Jerusalem, Israel

5Clinical Virology Unit, Hadassah Hebrew University Medical Center, Jerusalem, Israel

6Maccabi Healthcare Services, Rehovot, Israel

7Department of Obstetrics and Gynecology, IRCCS Policlinico San Matteo Foundation and University of Pavia, Pavia, Italy

8Grand Israel National Center for Personalized Medicine, Weizmann Institute of Science, Rehovot, Israel

9Info-CORE, Bioinformatics Unit of the I-CORE, The Hebrew University of Jerusalem, Jerusalem, Israel

10School of Computer Science and Engineering, The Hebrew University of Jerusalem, Jerusalem, Israel

11Faculty of Medicine, The Hebrew University of Jerusalem, Jerusalem, Israel

12Department of Speech and Hearing, Hadassah Hebrew University Medical Center, Jerusalem, Israel

13Department of Biochemistry, The Hebrew University of Jerusalem, Jerusalem, Israel

Find articles by Zavattoni, M. in: JCI | PubMed | Google Scholar

1Clinical Virology Unit, The Hebrew University of Jerusalem, Jerusalem, Israel

2Department of Obstetrics and Gynecology, Hadassah Hebrew University Medical Center, Jerusalem, Israel

3Department of Microbiology and Virology, IRCCS Policlinico San Matteo Foundation and University of Pavia, Pavia, Italy

4Department of Pathology, Hadassah Hebrew University Medical Center, Jerusalem, Israel

5Clinical Virology Unit, Hadassah Hebrew University Medical Center, Jerusalem, Israel

6Maccabi Healthcare Services, Rehovot, Israel

7Department of Obstetrics and Gynecology, IRCCS Policlinico San Matteo Foundation and University of Pavia, Pavia, Italy

8Grand Israel National Center for Personalized Medicine, Weizmann Institute of Science, Rehovot, Israel

9Info-CORE, Bioinformatics Unit of the I-CORE, The Hebrew University of Jerusalem, Jerusalem, Israel

10School of Computer Science and Engineering, The Hebrew University of Jerusalem, Jerusalem, Israel

11Faculty of Medicine, The Hebrew University of Jerusalem, Jerusalem, Israel

12Department of Speech and Hearing, Hadassah Hebrew University Medical Center, Jerusalem, Israel

13Department of Biochemistry, The Hebrew University of Jerusalem, Jerusalem, Israel

Find articles by Bdolah-Abram, T. in: JCI | PubMed | Google Scholar

1Clinical Virology Unit, The Hebrew University of Jerusalem, Jerusalem, Israel

2Department of Obstetrics and Gynecology, Hadassah Hebrew University Medical Center, Jerusalem, Israel

3Department of Microbiology and Virology, IRCCS Policlinico San Matteo Foundation and University of Pavia, Pavia, Italy

4Department of Pathology, Hadassah Hebrew University Medical Center, Jerusalem, Israel

5Clinical Virology Unit, Hadassah Hebrew University Medical Center, Jerusalem, Israel

6Maccabi Healthcare Services, Rehovot, Israel

7Department of Obstetrics and Gynecology, IRCCS Policlinico San Matteo Foundation and University of Pavia, Pavia, Italy

8Grand Israel National Center for Personalized Medicine, Weizmann Institute of Science, Rehovot, Israel

9Info-CORE, Bioinformatics Unit of the I-CORE, The Hebrew University of Jerusalem, Jerusalem, Israel

10School of Computer Science and Engineering, The Hebrew University of Jerusalem, Jerusalem, Israel

11Faculty of Medicine, The Hebrew University of Jerusalem, Jerusalem, Israel

12Department of Speech and Hearing, Hadassah Hebrew University Medical Center, Jerusalem, Israel

13Department of Biochemistry, The Hebrew University of Jerusalem, Jerusalem, Israel

Find articles by Baldanti, F. in: JCI | PubMed | Google Scholar

1Clinical Virology Unit, The Hebrew University of Jerusalem, Jerusalem, Israel

2Department of Obstetrics and Gynecology, Hadassah Hebrew University Medical Center, Jerusalem, Israel

3Department of Microbiology and Virology, IRCCS Policlinico San Matteo Foundation and University of Pavia, Pavia, Italy

4Department of Pathology, Hadassah Hebrew University Medical Center, Jerusalem, Israel

5Clinical Virology Unit, Hadassah Hebrew University Medical Center, Jerusalem, Israel

6Maccabi Healthcare Services, Rehovot, Israel

7Department of Obstetrics and Gynecology, IRCCS Policlinico San Matteo Foundation and University of Pavia, Pavia, Italy

8Grand Israel National Center for Personalized Medicine, Weizmann Institute of Science, Rehovot, Israel

9Info-CORE, Bioinformatics Unit of the I-CORE, The Hebrew University of Jerusalem, Jerusalem, Israel

10School of Computer Science and Engineering, The Hebrew University of Jerusalem, Jerusalem, Israel

11Faculty of Medicine, The Hebrew University of Jerusalem, Jerusalem, Israel

12Department of Speech and Hearing, Hadassah Hebrew University Medical Center, Jerusalem, Israel

13Department of Biochemistry, The Hebrew University of Jerusalem, Jerusalem, Israel

Find articles by Geal-Dor, M. in: JCI | PubMed | Google Scholar |

1Clinical Virology Unit, The Hebrew University of Jerusalem, Jerusalem, Israel

2Department of Obstetrics and Gynecology, Hadassah Hebrew University Medical Center, Jerusalem, Israel

3Department of Microbiology and Virology, IRCCS Policlinico San Matteo Foundation and University of Pavia, Pavia, Italy

4Department of Pathology, Hadassah Hebrew University Medical Center, Jerusalem, Israel

5Clinical Virology Unit, Hadassah Hebrew University Medical Center, Jerusalem, Israel

6Maccabi Healthcare Services, Rehovot, Israel

7Department of Obstetrics and Gynecology, IRCCS Policlinico San Matteo Foundation and University of Pavia, Pavia, Italy

8Grand Israel National Center for Personalized Medicine, Weizmann Institute of Science, Rehovot, Israel

9Info-CORE, Bioinformatics Unit of the I-CORE, The Hebrew University of Jerusalem, Jerusalem, Israel

10School of Computer Science and Engineering, The Hebrew University of Jerusalem, Jerusalem, Israel

11Faculty of Medicine, The Hebrew University of Jerusalem, Jerusalem, Israel

12Department of Speech and Hearing, Hadassah Hebrew University Medical Center, Jerusalem, Israel

13Department of Biochemistry, The Hebrew University of Jerusalem, Jerusalem, Israel

Find articles by Zakay-Rones, Z. in: JCI | PubMed | Google Scholar

1Clinical Virology Unit, The Hebrew University of Jerusalem, Jerusalem, Israel

2Department of Obstetrics and Gynecology, Hadassah Hebrew University Medical Center, Jerusalem, Israel

3Department of Microbiology and Virology, IRCCS Policlinico San Matteo Foundation and University of Pavia, Pavia, Italy

4Department of Pathology, Hadassah Hebrew University Medical Center, Jerusalem, Israel

5Clinical Virology Unit, Hadassah Hebrew University Medical Center, Jerusalem, Israel

6Maccabi Healthcare Services, Rehovot, Israel

7Department of Obstetrics and Gynecology, IRCCS Policlinico San Matteo Foundation and University of Pavia, Pavia, Italy

8Grand Israel National Center for Personalized Medicine, Weizmann Institute of Science, Rehovot, Israel

9Info-CORE, Bioinformatics Unit of the I-CORE, The Hebrew University of Jerusalem, Jerusalem, Israel

10School of Computer Science and Engineering, The Hebrew University of Jerusalem, Jerusalem, Israel

11Faculty of Medicine, The Hebrew University of Jerusalem, Jerusalem, Israel

12Department of Speech and Hearing, Hadassah Hebrew University Medical Center, Jerusalem, Israel

13Department of Biochemistry, The Hebrew University of Jerusalem, Jerusalem, Israel

Find articles by Yanai, N. in: JCI | PubMed | Google Scholar

1Clinical Virology Unit, The Hebrew University of Jerusalem, Jerusalem, Israel

2Department of Obstetrics and Gynecology, Hadassah Hebrew University Medical Center, Jerusalem, Israel

3Department of Microbiology and Virology, IRCCS Policlinico San Matteo Foundation and University of Pavia, Pavia, Italy

4Department of Pathology, Hadassah Hebrew University Medical Center, Jerusalem, Israel

5Clinical Virology Unit, Hadassah Hebrew University Medical Center, Jerusalem, Israel

6Maccabi Healthcare Services, Rehovot, Israel

7Department of Obstetrics and Gynecology, IRCCS Policlinico San Matteo Foundation and University of Pavia, Pavia, Italy

8Grand Israel National Center for Personalized Medicine, Weizmann Institute of Science, Rehovot, Israel

9Info-CORE, Bioinformatics Unit of the I-CORE, The Hebrew University of Jerusalem, Jerusalem, Israel

10School of Computer Science and Engineering, The Hebrew University of Jerusalem, Jerusalem, Israel

11Faculty of Medicine, The Hebrew University of Jerusalem, Jerusalem, Israel

12Department of Speech and Hearing, Hadassah Hebrew University Medical Center, Jerusalem, Israel

13Department of Biochemistry, The Hebrew University of Jerusalem, Jerusalem, Israel

Find articles by Yagel, S. in: JCI | PubMed | Google Scholar

1Clinical Virology Unit, The Hebrew University of Jerusalem, Jerusalem, Israel

2Department of Obstetrics and Gynecology, Hadassah Hebrew University Medical Center, Jerusalem, Israel

3Department of Microbiology and Virology, IRCCS Policlinico San Matteo Foundation and University of Pavia, Pavia, Italy

4Department of Pathology, Hadassah Hebrew University Medical Center, Jerusalem, Israel

5Clinical Virology Unit, Hadassah Hebrew University Medical Center, Jerusalem, Israel

6Maccabi Healthcare Services, Rehovot, Israel

7Department of Obstetrics and Gynecology, IRCCS Policlinico San Matteo Foundation and University of Pavia, Pavia, Italy

8Grand Israel National Center for Personalized Medicine, Weizmann Institute of Science, Rehovot, Israel

9Info-CORE, Bioinformatics Unit of the I-CORE, The Hebrew University of Jerusalem, Jerusalem, Israel

10School of Computer Science and Engineering, The Hebrew University of Jerusalem, Jerusalem, Israel

11Faculty of Medicine, The Hebrew University of Jerusalem, Jerusalem, Israel

12Department of Speech and Hearing, Hadassah Hebrew University Medical Center, Jerusalem, Israel

13Department of Biochemistry, The Hebrew University of Jerusalem, Jerusalem, Israel

Find articles by Panet, A. in: JCI | PubMed | Google Scholar

1Clinical Virology Unit, The Hebrew University of Jerusalem, Jerusalem, Israel

2Department of Obstetrics and Gynecology, Hadassah Hebrew University Medical Center, Jerusalem, Israel

3Department of Microbiology and Virology, IRCCS Policlinico San Matteo Foundation and University of Pavia, Pavia, Italy

4Department of Pathology, Hadassah Hebrew University Medical Center, Jerusalem, Israel

5Clinical Virology Unit, Hadassah Hebrew University Medical Center, Jerusalem, Israel

6Maccabi Healthcare Services, Rehovot, Israel

7Department of Obstetrics and Gynecology, IRCCS Policlinico San Matteo Foundation and University of Pavia, Pavia, Italy

8Grand Israel National Center for Personalized Medicine, Weizmann Institute of Science, Rehovot, Israel

9Info-CORE, Bioinformatics Unit of the I-CORE, The Hebrew University of Jerusalem, Jerusalem, Israel

10School of Computer Science and Engineering, The Hebrew University of Jerusalem, Jerusalem, Israel

11Faculty of Medicine, The Hebrew University of Jerusalem, Jerusalem, Israel

12Department of Speech and Hearing, Hadassah Hebrew University Medical Center, Jerusalem, Israel

13Department of Biochemistry, The Hebrew University of Jerusalem, Jerusalem, Israel

Find articles by Wolf, D. in: JCI | PubMed | Google Scholar

Published April 19, 2022 - More info

J Clin Invest. https://doi.org/10.1172/JCI157415.
Copyright © 2022, Vorontsov et al. This work is licensed under the Creative Commons Attribution 4.0 International License. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
Published April 19, 2022 - Version history
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Abstract

BACKGROUND. Cytomegalovirus (CMV) is the most common intrauterine infection, leading to infant brain damage. Prognostic assessment of CMV-infected fetuses has remained an ongoing challenge in prenatal care, in the absence of established prenatal biomarkers of congenital CMV (cCMV) infection severity. We aimed to identify prognostic biomarkers of cCMV-related fetal brain injury.

METHODS. Global proteome analysis was performed in mid-gestation amniotic fluid samples, comparing fetuses with severe cCMV to asymptomatic CMV-infected fetuses. The levels of selected differentially-excreted proteins were further determined by specific immunoassays.

RESULTS. Employing unbiased proteome analysis in a discovery cohort, we identified amniotic fluid proteins related to inflammation and neurological disease pathways, which demonstrated distinct abundance in fetuses with severe cCMV. Amniotic fluid levels of two of these proteins - the immunomodulatory proteins chemerin and galectin-3-binding-protein - were highly predictive of the severity of cCMV in an independent validation cohort, differentiating between fetuses with severe (N=17) and asymptomatic (N=26) cCMV, with 100-93.8% positive predictive value, and 92.9-92.6% negative predictive value (for chemerin - galectin-3-binding-protein, respectively).

CONCLUSION. Analysis of chemerin and galectin-3-binding-protein in mid-gestation amniotic fluids could be employed in the clinical setting to profoundly improve the prognostic assessment of CMV-infected fetuses.

TRIAL REGISTRATION. NA

FUNDING. Israel Science Foundation; Research Fund - Hadassah Medical Organization.

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